BBa_K2039002
type: ComponentDefinition
persistent identity: BBa K2039002
display id: BBa_K2039002
version: 1
was generated by: iGEM to SBOL conversion
description: Dimerization system FRB/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11)
created: 2016-10-12T11:00:00Z
modified: 2016-10-14T09:02:50Z
mutable provenance: Composite part
owned by: james
owned by: myers
mutable description: This part is the association of part K2039000 and K2039001. The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria. FRB* protein is linked to GFP 11. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).
discontinued: false
dominant: false
experience: experience/None
group u list: _2506_
m user id: 31202
owner id: 31112
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Codon optomization for E. coli
star: false
creator: Coline Sivelle
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K2039001
component: BBa_K2039000
sequence annotation: BBa_K2039001
sequence annotation: BBa_K2039000
sequence: https://synbiohub.org/public/igem/BBa_K2039002_sequence/1

BBa_K2039001
type: Component
persistent identity: component2506152
display id: component2506152
version: 1
top level: BBa_K2039002
definition: BBa_K2039001
access: public

BBa_K2039000
type: Component
persistent identity: component2506165
display id: component2506165
version: 1
top level: BBa_K2039002
definition: BBa_K2039000
access: public

BBa_K2039001
type: SequenceAnnotation
persistent identity: annotation2506152
display id: annotation2506152
version: 1
top level: BBa_K2039002
location: https://synbiohub.org/public/igem/BBa_K2039002/annotation2506152/range2506152/1
component: BBa_K2039001

https://synbiohub.org/public/igem/BBa_K2039002/annotation2506152/range2506152/1
type: Range
persistent identity: range2506152
display id: range2506152
version: 1
top level: BBa_K2039002
direction: direction/reverse
start: 1
end: 701
orientation: inline

BBa_K2039000
type: SequenceAnnotation
persistent identity: annotation2506165
display id: annotation2506165
version: 1
top level: BBa_K2039002
location: https://synbiohub.org/public/igem/BBa_K2039002/annotation2506165/range2506165/1
component: BBa_K2039000

https://synbiohub.org/public/igem/BBa_K2039002/annotation2506165/range2506165/1
type: Range
persistent identity: range2506165
display id: range2506165
version: 1
top level: BBa_K2039002
direction: direction/reverse
start: 710
end: 1440
orientation: inline

BBa_K2039000
type: ComponentDefinition
persistent identity: BBa K2039000
display id: BBa_K2039000
version: 1
was generated by: iGEM to SBOL conversion
description: Part expressing the dimerization protein FKBP and GFP10 (subunit of tripartite GFP)
created: 2016-10-11T11:00:00Z
modified: 2016-10-14T12:58:32Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part is the complementary part of Bba_K2039001. The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been optimized in order to be expressed in bacteria: mutations (T2098L, K2095P, W2101F) for dimerization with rapalog and optimization by Jcat plateforme. FKBP protein is linked to GFP 10. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).
discontinued: false
dominant: false
experience: experience/None
group u list: _2506_
m user id: 31112
owner id: 31202
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No issues
star: false
creator: Maxence Pfeiffer
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_J23119
component: BBa_B0015
component: BBa_K2039003
sequence annotation: BBa_J23119
sequence annotation: BBa_B0030
sequence annotation: BBa_K2039003
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K2039000_sequence/1

BBa_B0030
type: Component
persistent identity: component2506108
display id: component2506108
version: 1
top level: BBa_K2039000
definition: BBa_B0030
access: public

BBa_J23119
type: Component
persistent identity: component2506106
display id: component2506106
version: 1
top level: BBa_K2039000
definition: BBa_J23119
access: public

BBa_B0015
type: Component
persistent identity: component2506117
display id: component2506117
version: 1
top level: BBa_K2039000
definition: BBa_B0015
access: public

BBa_K2039003
type: Component
persistent identity: component2506110
display id: component2506110
version: 1
top level: BBa_K2039000
definition: BBa_K2039003
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2506106
display id: annotation2506106
version: 1
top level: BBa_K2039000
location: https://synbiohub.org/public/igem/BBa_K2039000/annotation2506106/range2506106/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K2039000/annotation2506106/range2506106/1
type: Range
persistent identity: range2506106
display id: range2506106
version: 1
top level: BBa_K2039000
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2506108
display id: annotation2506108
version: 1
top level: BBa_K2039000
location: https://synbiohub.org/public/igem/BBa_K2039000/annotation2506108/range2506108/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2039000/annotation2506108/range2506108/1
type: Range
persistent identity: range2506108
display id: range2506108
version: 1
top level: BBa_K2039000
direction: direction/forward
start: 44
end: 58
orientation: inline

BBa_K2039003
type: SequenceAnnotation
persistent identity: annotation2506110
display id: annotation2506110
version: 1
top level: BBa_K2039000
location: https://synbiohub.org/public/igem/BBa_K2039000/annotation2506110/range2506110/1
component: BBa_K2039003

https://synbiohub.org/public/igem/BBa_K2039000/annotation2506110/range2506110/1
type: Range
persistent identity: range2506110
display id: range2506110
version: 1
top level: BBa_K2039000
direction: direction/reverse
start: 67
end: 594
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2506117
display id: annotation2506117
version: 1
top level: BBa_K2039000
location: https://synbiohub.org/public/igem/BBa_K2039000/annotation2506117/range2506117/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2039000/annotation2506117/range2506117/1
type: Range
persistent identity: range2506117
display id: range2506117
version: 1
top level: BBa_K2039000
direction: direction/reverse
start: 603
end: 731
orientation: inline

BBa_K2039001
type: ComponentDefinition
persistent identity: BBa K2039001
display id: BBa_K2039001
version: 1
was generated by: iGEM to SBOL conversion
description: Part expressing the dimerization protein FRB* and GFP11 (subunit of tripartite GFP)
created: 2016-10-12T11:00:00Z
modified: 2016-10-14T01:00:04Z
mutable provenance: Gblock
owned by: james
owned by: myers
mutable description: This part is the complementary part of Bba_K2039000. The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria. FRB* protein is linked to GFP 11. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9). Sequence and Features
discontinued: false
dominant: false
experience: experience/None
group u list: _2506_
m user id: 31112
owner id: 31112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The mutations introduced are : T2098L, K2095P, W2101F.
star: false
creator: Coline Sivelle
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J23119
component: BBa_B0015
component: BBa_K2039004
component: BBa_B0030
sequence annotation: BBa_J23119
sequence annotation: BBa_B0030
sequence annotation: BBa_K2039004
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K2039001_sequence/1

BBa_J23119
type: Component
persistent identity: component2506122
display id: component2506122
version: 1
top level: BBa_K2039001
definition: BBa_J23119
access: public

BBa_B0015
type: Component
persistent identity: component2506133
display id: component2506133
version: 1
top level: BBa_K2039001
definition: BBa_B0015
access: public

BBa_K2039004
type: Component
persistent identity: component2506126
display id: component2506126
version: 1
top level: BBa_K2039001
definition: BBa_K2039004
access: public

BBa_B0030
type: Component
persistent identity: component2506124
display id: component2506124
version: 1
top level: BBa_K2039001
definition: BBa_B0030
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2506122
display id: annotation2506122
version: 1
top level: BBa_K2039001
location: https://synbiohub.org/public/igem/BBa_K2039001/annotation2506122/range2506122/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K2039001/annotation2506122/range2506122/1
type: Range
persistent identity: range2506122
display id: range2506122
version: 1
top level: BBa_K2039001
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2506124
display id: annotation2506124
version: 1
top level: BBa_K2039001
location: https://synbiohub.org/public/igem/BBa_K2039001/annotation2506124/range2506124/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2039001/annotation2506124/range2506124/1
type: Range
persistent identity: range2506124
display id: range2506124
version: 1
top level: BBa_K2039001
direction: direction/forward
start: 44
end: 58
orientation: inline

BBa_K2039004
type: SequenceAnnotation
persistent identity: annotation2506126
display id: annotation2506126
version: 1
top level: BBa_K2039001
location: https://synbiohub.org/public/igem/BBa_K2039001/annotation2506126/range2506126/1
component: BBa_K2039004

https://synbiohub.org/public/igem/BBa_K2039001/annotation2506126/range2506126/1
type: Range
persistent identity: range2506126
display id: range2506126
version: 1
top level: BBa_K2039001
direction: direction/reverse
start: 67
end: 564
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2506133
display id: annotation2506133
version: 1
top level: BBa_K2039001
location: https://synbiohub.org/public/igem/BBa_K2039001/annotation2506133/range2506133/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2039001/annotation2506133/range2506133/1
type: Range
persistent identity: range2506133
display id: range2506133
version: 1
top level: BBa_K2039001
direction: direction/reverse
start: 573
end: 701
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_K2039003
type: ComponentDefinition
persistent identity: BBa K2039003
display id: BBa_K2039003
version: 1
was generated by: iGEM to SBOL conversion
description: Coding sequences of proteins FKBP and GFP10 (subunit of tripartite GFP)
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:57:36Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This part is the complementary part of Bba_K2039004. The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the medium. It is particularly useful for protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria. FKBP protein is linked to GFP 10. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).
discontinued: false
dominant: false
experience: experience/None
group u list: _2506_
m user id: 31112
owner id: 31202
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No specific considerations
star: false
creator: Maxence Pfeiffer
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K2039003_sequence/1

BBa_K2039004
type: ComponentDefinition
persistent identity: BBa K2039004
display id: BBa_K2039004
version: 1
was generated by: iGEM to SBOL conversion
description: Coding sequences of proteins FRB* and GFP11 (subunit of tripartite GFP)
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T08:39:06Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This part is the complementary part of Bba_K2039003. The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the medium. It is particularly useful for protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria. FKBP protein is linked to GFP 10. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).
discontinued: false
dominant: false
group u list: _2506_
m user id: 31202
owner id: 31202
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No specific considerations
star: false
creator: Maxence Pfeiffer
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K2039004_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2039002_sequence/1
type: Sequence
persistent identity: BBa K2039002 sequence
display id: BBa_K2039002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgatcctgtggcacgaaatgtggcacgaaggtctggaagaagcttctcgtctgtacttcggtgaacgtaacgttaaaggtatgttcgaagttctggaaccgctgcacgctatgatggaacgtggtccgcagaccctgaaagaaacctctttcaaccaggcttacggtcgtgacctgatggaagctcaggaatggtgccgtaaatacatgaaatctggtaacgttccggacctgctgcaagctttcgacctgtactaccacgttttccgtcgtatctctaaagagctcggatccgatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcgaaaaacgcgatcatatggtgctgctggaatatgtgaccgcggcgggcattaccgatgcgagctaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgatgggtgttcaggttgaaaccatctctccgggtgacggtcgtaccttcccgaaacgtggtcagacctgcgttgttcactacaccggtatgctggaagacggtaaaaaattcgactcttctcgtgaccgtaacaaaccgttcaaattcaccctgggtaaacaggaagttatccgtggttgggaagaaggtgttgctcagatgtctgttggtcagcgtgctaaactgatcatctcttctgactacgcttacggtgctaccggtcacccgggtatcatcccgccgcacgctaccctggttttcgacgttgaactgctgaaactggaagatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcatggatctgccggatgatcattatctgagcacccagaccattctgagcaaagatctgaactaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2039000_sequence/1
type: Sequence
persistent identity: BBa K2039000 sequence
display id: BBa_K2039000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgatgggtgttcaggttgaaaccatctctccgggtgacggtcgtaccttcccgaaacgtggtcagacctgcgttgttcactacaccggtatgctggaagacggtaaaaaattcgactcttctcgtgaccgtaacaaaccgttcaaattcaccctgggtaaacaggaagttatccgtggttgggaagaaggtgttgctcagatgtctgttggtcagcgtgctaaactgatcatctcttctgactacgcttacggtgctaccggtcacccgggtatcatcccgccgcacgctaccctggttttcgacgttgaactgctgaaactggaagatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcatggatctgccggatgatcattatctgagcacccagaccattctgagcaaagatctgaactaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2039001_sequence/1
type: Sequence
persistent identity: BBa K2039001 sequence
display id: BBa_K2039001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgatcctgtggcacgaaatgtggcacgaaggtctggaagaagcttctcgtctgtacttcggtgaacgtaacgttaaaggtatgttcgaagttctggaaccgctgcacgctatgatggaacgtggtccgcagaccctgaaagaaacctctttcaaccaggcttacggtcgtgacctgatggaagctcaggaatggtgccgtaaatacatgaaatctggtaacgttccggacctgctgcaagctttcgacctgtactaccacgttttccgtcgtatctctaaagagctcggatccgatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcgaaaaacgcgatcatatggtgctgctggaatatgtgaccgcggcgggcattaccgatgcgagctaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2039003_sequence/1
type: Sequence
persistent identity: BBa K2039003 sequence
display id: BBa_K2039003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagatgatgggtgttcaggttgaaaccatctctccgggtgacggtcgtaccttcccgaaacgtggtcagacctgcgttgttcactacaccggtatgctggaagacggtaaaaaattcgactcttctcgtgaccgtaacaaaccgttcaaattcaccctgggtaaacaggaagttatccgtggttgggaagaaggtgttgctcagatgtctgttggtcagcgtgctaaactgatcatctcttctgactacgcttacggtgctaccggtcacccgggtatcatcccgccgcacgctaccctggttttcgacgttgaactgctgaaactggaagatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcatggatctgccggatgatcattatctgagcacccagaccattctgagcaaagatctgaactaataacgctgatagtgctagtgtagatcgctactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2039004_sequence/1
type: Sequence
persistent identity: BBa K2039004 sequence
display id: BBa_K2039004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagatgatcctgtggcacgaaatgtggcacgaaggtctggaagaagcttctcgtctgtacttcggtgaacgtaacgttaaaggtatgttcgaagttctggaaccgctgcacgctatgatggaacgtggtccgcagaccctgaaagaaacctctttcaaccaggcttacggtcgtgacctgatggaagctcaggaatggtgccgtaaatacatgaaatctggtaacgttccggacctgctgcaagctttcgacctgtactaccacgttttccgtcgtatctctaaagagctcggatccgatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcgaaaaacgcgatcatatggtgctgctggaatatgtgaccgcggcgggcattaccgatgcgagctaataacgctgatagtgctagtgtagatcgctactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z