LOCUS       BBa_K2039007            2284 bp    DNA     linear       22-Nov-2024
DEFINITION  Dimerization system FRB/FKBP linked to tripartite GFP (GFP 10, GFP 
            11 & GFP 1.9)
ACCESSION   BBa_K2039007
VERSION     BBa_K2039007.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    67..564
                     /label=BBa_K2039004
     stem_loop       584..627
                     /label=stem_loop
     misc_feature    573..652
                     /label=BBa_B0010
     terminator      573..652
                     /label=BBa_B0010
     stem_loop       668..687
                     /label=T7 TE
     misc_feature    694..694
                     /label=stop
     polyA_site      688..701
                     /label=polya
     misc_feature    661..701
                     /label=BBa_B0012
     terminator      661..701
                     /label=BBa_B0012
     terminator      573..701
                     /label=BBa_B0015
     misc_feature    1..701
                     /label=BBa_K2039001
     promoter        710..744
                     /label=BBa_J23119
     RBS             753..767
                     /label=RBS-1\Strong
     RBS             760..764
                     /label=RBS
     misc_feature    753..767
                     /label=BBa_B0030
     RBS             753..767
                     /label=BBa_B0030
     misc_feature    776..1303
                     /label=BBa_K2039003
     stem_loop       1323..1366
                     /label=stem_loop
     misc_feature    1312..1391
                     /label=BBa_B0010
     terminator      1312..1391
                     /label=BBa_B0010
     stem_loop       1407..1426
                     /label=T7 TE
     misc_feature    1433..1433
                     /label=stop
     polyA_site      1427..1440
                     /label=polya
     misc_feature    1400..1440
                     /label=BBa_B0012
     terminator      1400..1440
                     /label=BBa_B0012
     terminator      1312..1440
                     /label=BBa_B0015
     misc_feature    710..1440
                     /label=BBa_K2039000
     misc_feature    1..1440
                     /label=BBa_K2039002
     promoter        1449..1483
                     /label=BBa_J23119
     RBS             1492..1506
                     /label=RBS-1\Strong
     RBS             1499..1503
                     /label=RBS
     misc_feature    1492..1506
                     /label=BBa_B0030
     RBS             1492..1506
                     /label=BBa_B0030
     misc_feature    1515..2147
                     /label=BBa_K2039005
     stem_loop       2167..2210
                     /label=stem_loop
     misc_feature    2156..2235
                     /label=BBa_B0010
     terminator      2156..2235
                     /label=BBa_B0010
     stem_loop       2251..2270
                     /label=T7 TE
     misc_feature    2277..2277
                     /label=stop
     polyA_site      2271..2284
                     /label=polya
     misc_feature    2244..2284
                     /label=BBa_B0012
     terminator      2244..2284
                     /label=BBa_B0012
     terminator      2156..2284
                     /label=BBa_B0015
     misc_feature    1449..2284
                     /label=BBa_K2039006
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagattaaag aggagaaata
       61 ctagagtact agatgatcct gtggcacgaa atgtggcacg aaggtctgga agaagcttct
      121 cgtctgtact tcggtgaacg taacgttaaa ggtatgttcg aagttctgga accgctgcac
      181 gctatgatgg aacgtggtcc gcagaccctg aaagaaacct ctttcaacca ggcttacggt
      241 cgtgacctga tggaagctca ggaatggtgc cgtaaataca tgaaatctgg taacgttccg
      301 gacctgctgc aagctttcga cctgtactac cacgttttcc gtcgtatctc taaagagctc
      361 ggatccgatg taggaggtgg aggtagtgaa ggaggaggta gcggtggtcc gggaagcgca
      421 ggcgaaggca gcgcggtagg aggttctgct ggaggtggca gcgaaaaacg cgatcatatg
      481 gtgctgctgg aatatgtgac cgcggcgggc attaccgatg cgagctaata acgctgatag
      541 tgctagtgta gatcgctact agagtactag agccaggcat caaataaaac gaaaggctca
      601 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      661 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt tgacagctag
      721 ctcagtccta ggtataatgc tagctactag agattaaaga ggagaaatac tagagtacta
      781 gatgatgggt gttcaggttg aaaccatctc tccgggtgac ggtcgtacct tcccgaaacg
      841 tggtcagacc tgcgttgttc actacaccgg tatgctggaa gacggtaaaa aattcgactc
      901 ttctcgtgac cgtaacaaac cgttcaaatt caccctgggt aaacaggaag ttatccgtgg
      961 ttgggaagaa ggtgttgctc agatgtctgt tggtcagcgt gctaaactga tcatctcttc
     1021 tgactacgct tacggtgcta ccggtcaccc gggtatcatc ccgccgcacg ctaccctggt
     1081 tttcgacgtt gaactgctga aactggaaga tgtaggaggt ggaggtagtg aaggaggagg
     1141 tagcggtggt ccgggaagcg caggcgaagg cagcgcggta ggaggttctg ctggaggtgg
     1201 cagcatggat ctgccggatg atcattatct gagcacccag accattctga gcaaagatct
     1261 gaactaataa cgctgatagt gctagtgtag atcgctacta gagtactaga gccaggcatc
     1321 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1381 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     1441 tactagagtt gacagctagc tcagtcctag gtataatgct agctactaga gattaaagag
     1501 gagaaatact agagtactag atgcgcaaag gcgaagaact gtttaccggc gtggtgccga
     1561 ttctgattga actggatggc gatgtgaacg gccataaatt ttttgtgcgc ggcgaaggcg
     1621 aaggcgatgc gaccattggc aaactgagcc tgaaatttat ttgcaccacc ggcaaactgc
     1681 cggtgccgtg gccgaccctg gtgaccaccc tgacctatgg cgtgcagtgc tttagccgct
     1741 atccggatca tatgaaacgc catgattttt ttaaaagcgc gatgccggaa ggctatgtgc
     1801 aggaacgcac catttatttt aaagatgatg gcacctataa aacccgcgcg gaagtgaaat
     1861 ttgaaggcga taccctggtg aaccgcattg aactgaaagg cattgatttt aaagaagatg
     1921 gcaacattct gggccataaa ctggaatata actttaacag ccataaagtg tatattaccg
     1981 cggataaaca gaacaacggc attaaagcga actttaccat tcgccataac gtggaagatg
     2041 gcagcgtgca gctggcggat cattatcagc agaacacccc gattggcgat ggcccggtgc
     2101 tgctgccgta ataacgctga tagtgctagt gtagatcgct actagagtac tagagccagg
     2161 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2221 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2281 tata
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