BBa_K2039007 1 BBa_K2039007 Dimerization system FRB/FKBP linked to tripartite GFP (GFP 10, GFP 11 & GFP 1.9) 2016-10-13T11:00:00Z 2016-10-14T10:46:42Z Synthetic This part is the association of part K2039002 and K2039006. The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9). false false _2506_ 31202 31202 9 false No specific consideration false Maxence Pfeiffer component2506998 1 BBa_K2039006 component2506985 1 BBa_K2039002 annotation2506998 1 BBa_K2039006 range2506998 1 1449 2284 annotation2506985 1 BBa_K2039002 range2506985 1 1 1440 BBa_K2039005 1 BBa_K2039005 Coding sequences of protein GFP 1.9 (subunit of tripartite GFP) 2016-10-13T11:00:00Z 2016-10-14T10:15:40Z Synthetic This part is the complementary part of Bba_K2039004 and Bba_K203003. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9). false false _2506_ 31202 31202 9 false No specific consideration false Maxence Pfeiffer BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K2039003 1 BBa_K2039003 Coding sequences of proteins FKBP and GFP10 (subunit of tripartite GFP) 2016-10-13T11:00:00Z 2016-10-14T12:57:36Z Synthetic. This part is the complementary part of Bba_K2039004. The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the medium. It is particularly useful for protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria. FKBP protein is linked to GFP 10. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9). false false _2506_ 31112 31202 9 Not in stock false No specific considerations false Maxence Pfeiffer BBa_K2039006 1 BBa_K2039006 Part expressing the protein GFP 1.9 (subunit of tripartite GFP) 2016-10-13T11:00:00Z 2016-10-14T10:18:53Z Synthetic This part is the complementary part of Bba_K2039000 and Bba_K2039001. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9). false false _2506_ 31202 31202 9 false No specific consideration false Maxence Pfeiffer component2506839 1 BBa_J23119 component2506841 1 BBa_B0030 component2506843 1 BBa_K2039005 component2506850 1 BBa_B0015 annotation2506850 1 BBa_B0015 range2506850 1 708 836 annotation2506839 1 BBa_J23119 range2506839 1 1 35 annotation2506841 1 BBa_B0030 range2506841 1 44 58 annotation2506843 1 BBa_K2039005 range2506843 1 67 699 BBa_K2039004 1 BBa_K2039004 Coding sequences of proteins FRB* and GFP11 (subunit of tripartite GFP) 2016-10-13T11:00:00Z 2016-10-14T08:39:06Z Synthetic. This part is the complementary part of Bba_K2039003. The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the medium. It is particularly useful for protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria. FKBP protein is linked to GFP 10. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9). false false _2506_ 31202 31202 9 false No specific considerations false Maxence Pfeiffer BBa_K2039000 1 BBa_K2039000 Part expressing the dimerization protein FKBP and GFP10 (subunit of tripartite GFP) 2016-10-11T11:00:00Z 2016-10-14T12:58:32Z Synthetic This part is the complementary part of Bba_K2039001. The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been optimized in order to be expressed in bacteria: mutations (T2098L, K2095P, W2101F) for dimerization with rapalog and optimization by Jcat plateforme. FKBP protein is linked to GFP 10. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9). false false _2506_ 31112 31202 9 Not in stock false No issues false Maxence Pfeiffer component2506110 1 BBa_K2039003 component2506106 1 BBa_J23119 component2506108 1 BBa_B0030 component2506117 1 BBa_B0015 annotation2506106 1 BBa_J23119 range2506106 1 1 35 annotation2506117 1 BBa_B0015 range2506117 1 603 731 annotation2506108 1 BBa_B0030 range2506108 1 44 58 annotation2506110 1 BBa_K2039003 range2506110 1 67 594 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K2039001 1 BBa_K2039001 Part expressing the dimerization protein FRB* and GFP11 (subunit of tripartite GFP) 2016-10-12T11:00:00Z 2016-10-14T01:00:04Z Gblock This part is the complementary part of Bba_K2039000. The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria. FRB* protein is linked to GFP 11. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9). Sequence and Features false false _2506_ 31112 31112 9 Not in stock false The mutations introduced are : T2098L, K2095P, W2101F. false Coline Sivelle component2506126 1 BBa_K2039004 component2506133 1 BBa_B0015 component2506124 1 BBa_B0030 component2506122 1 BBa_J23119 annotation2506122 1 BBa_J23119 range2506122 1 1 35 annotation2506126 1 BBa_K2039004 range2506126 1 67 564 annotation2506124 1 BBa_B0030 range2506124 1 44 58 annotation2506133 1 BBa_B0015 range2506133 1 573 701 BBa_K2039002 1 BBa_K2039002 Dimerization system FRB/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11) 2016-10-12T11:00:00Z 2016-10-14T09:02:50Z Composite part This part is the association of part K2039000 and K2039001. The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria. FRB* protein is linked to GFP 11. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9). false false _2506_ 31202 31112 9 false Codon optomization for E. coli false Coline Sivelle component2506152 1 BBa_K2039001 component2506165 1 BBa_K2039000 annotation2506165 1 BBa_K2039000 range2506165 1 710 1440 annotation2506152 1 BBa_K2039001 range2506152 1 1 701 BBa_K2039002_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgatcctgtggcacgaaatgtggcacgaaggtctggaagaagcttctcgtctgtacttcggtgaacgtaacgttaaaggtatgttcgaagttctggaaccgctgcacgctatgatggaacgtggtccgcagaccctgaaagaaacctctttcaaccaggcttacggtcgtgacctgatggaagctcaggaatggtgccgtaaatacatgaaatctggtaacgttccggacctgctgcaagctttcgacctgtactaccacgttttccgtcgtatctctaaagagctcggatccgatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcgaaaaacgcgatcatatggtgctgctggaatatgtgaccgcggcgggcattaccgatgcgagctaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgatgggtgttcaggttgaaaccatctctccgggtgacggtcgtaccttcccgaaacgtggtcagacctgcgttgttcactacaccggtatgctggaagacggtaaaaaattcgactcttctcgtgaccgtaacaaaccgttcaaattcaccctgggtaaacaggaagttatccgtggttgggaagaaggtgttgctcagatgtctgttggtcagcgtgctaaactgatcatctcttctgactacgcttacggtgctaccggtcacccgggtatcatcccgccgcacgctaccctggttttcgacgttgaactgctgaaactggaagatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcatggatctgccggatgatcattatctgagcacccagaccattctgagcaaagatctgaactaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2039000_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgatgggtgttcaggttgaaaccatctctccgggtgacggtcgtaccttcccgaaacgtggtcagacctgcgttgttcactacaccggtatgctggaagacggtaaaaaattcgactcttctcgtgaccgtaacaaaccgttcaaattcaccctgggtaaacaggaagttatccgtggttgggaagaaggtgttgctcagatgtctgttggtcagcgtgctaaactgatcatctcttctgactacgcttacggtgctaccggtcacccgggtatcatcccgccgcacgctaccctggttttcgacgttgaactgctgaaactggaagatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcatggatctgccggatgatcattatctgagcacccagaccattctgagcaaagatctgaactaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2039006_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgcgcaaaggcgaagaactgtttaccggcgtggtgccgattctgattgaactggatggcgatgtgaacggccataaattttttgtgcgcggcgaaggcgaaggcgatgcgaccattggcaaactgagcctgaaatttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttagccgctatccggatcatatgaaacgccatgatttttttaaaagcgcgatgccggaaggctatgtgcaggaacgcaccatttattttaaagatgatggcacctataaaacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgattttaaagaagatggcaacattctgggccataaactggaatataactttaacagccataaagtgtatattaccgcggataaacagaacaacggcattaaagcgaactttaccattcgccataacgtggaagatggcagcgtgcagctggcggatcattatcagcagaacaccccgattggcgatggcccggtgctgctgccgtaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2039005_sequence 1 tactagatgcgcaaaggcgaagaactgtttaccggcgtggtgccgattctgattgaactggatggcgatgtgaacggccataaattttttgtgcgcggcgaaggcgaaggcgatgcgaccattggcaaactgagcctgaaatttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttagccgctatccggatcatatgaaacgccatgatttttttaaaagcgcgatgccggaaggctatgtgcaggaacgcaccatttattttaaagatgatggcacctataaaacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgattttaaagaagatggcaacattctgggccataaactggaatataactttaacagccataaagtgtatattaccgcggataaacagaacaacggcattaaagcgaactttaccattcgccataacgtggaagatggcagcgtgcagctggcggatcattatcagcagaacaccccgattggcgatggcccggtgctgctgccgtaataacgctgatagtgctagtgtagatcgctactagag BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2039004_sequence 1 tactagatgatcctgtggcacgaaatgtggcacgaaggtctggaagaagcttctcgtctgtacttcggtgaacgtaacgttaaaggtatgttcgaagttctggaaccgctgcacgctatgatggaacgtggtccgcagaccctgaaagaaacctctttcaaccaggcttacggtcgtgacctgatggaagctcaggaatggtgccgtaaatacatgaaatctggtaacgttccggacctgctgcaagctttcgacctgtactaccacgttttccgtcgtatctctaaagagctcggatccgatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcgaaaaacgcgatcatatggtgctgctggaatatgtgaccgcggcgggcattaccgatgcgagctaataacgctgatagtgctagtgtagatcgctactagag BBa_B0030_sequence 1 attaaagaggagaaa BBa_K2039007_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgatcctgtggcacgaaatgtggcacgaaggtctggaagaagcttctcgtctgtacttcggtgaacgtaacgttaaaggtatgttcgaagttctggaaccgctgcacgctatgatggaacgtggtccgcagaccctgaaagaaacctctttcaaccaggcttacggtcgtgacctgatggaagctcaggaatggtgccgtaaatacatgaaatctggtaacgttccggacctgctgcaagctttcgacctgtactaccacgttttccgtcgtatctctaaagagctcggatccgatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcgaaaaacgcgatcatatggtgctgctggaatatgtgaccgcggcgggcattaccgatgcgagctaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgatgggtgttcaggttgaaaccatctctccgggtgacggtcgtaccttcccgaaacgtggtcagacctgcgttgttcactacaccggtatgctggaagacggtaaaaaattcgactcttctcgtgaccgtaacaaaccgttcaaattcaccctgggtaaacaggaagttatccgtggttgggaagaaggtgttgctcagatgtctgttggtcagcgtgctaaactgatcatctcttctgactacgcttacggtgctaccggtcacccgggtatcatcccgccgcacgctaccctggttttcgacgttgaactgctgaaactggaagatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcatggatctgccggatgatcattatctgagcacccagaccattctgagcaaagatctgaactaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgcgcaaaggcgaagaactgtttaccggcgtggtgccgattctgattgaactggatggcgatgtgaacggccataaattttttgtgcgcggcgaaggcgaaggcgatgcgaccattggcaaactgagcctgaaatttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttagccgctatccggatcatatgaaacgccatgatttttttaaaagcgcgatgccggaaggctatgtgcaggaacgcaccatttattttaaagatgatggcacctataaaacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgattttaaagaagatggcaacattctgggccataaactggaatataactttaacagccataaagtgtatattaccgcggataaacagaacaacggcattaaagcgaactttaccattcgccataacgtggaagatggcagcgtgcagctggcggatcattatcagcagaacaccccgattggcgatggcccggtgctgctgccgtaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2039001_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgatcctgtggcacgaaatgtggcacgaaggtctggaagaagcttctcgtctgtacttcggtgaacgtaacgttaaaggtatgttcgaagttctggaaccgctgcacgctatgatggaacgtggtccgcagaccctgaaagaaacctctttcaaccaggcttacggtcgtgacctgatggaagctcaggaatggtgccgtaaatacatgaaatctggtaacgttccggacctgctgcaagctttcgacctgtactaccacgttttccgtcgtatctctaaagagctcggatccgatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcgaaaaacgcgatcatatggtgctgctggaatatgtgaccgcggcgggcattaccgatgcgagctaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2039003_sequence 1 tactagatgatgggtgttcaggttgaaaccatctctccgggtgacggtcgtaccttcccgaaacgtggtcagacctgcgttgttcactacaccggtatgctggaagacggtaaaaaattcgactcttctcgtgaccgtaacaaaccgttcaaattcaccctgggtaaacaggaagttatccgtggttgggaagaaggtgttgctcagatgtctgttggtcagcgtgctaaactgatcatctcttctgactacgcttacggtgctaccggtcacccgggtatcatcccgccgcacgctaccctggttttcgacgttgaactgctgaaactggaagatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcatggatctgccggatgatcattatctgagcacccagaccattctgagcaaagatctgaactaataacgctgatagtgctagtgtagatcgctactagag BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z