LOCUS       BBa_K204018             1057 bp    DNA     linear       22-Dec-2024
DEFINITION  ptetR+EspE
ACCESSION   BBa_K204018
VERSION     BBa_K204018.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..914
                     /label=EpsE Molecular Clutch Gene
     misc_feature    81..83
                     /label=start
     misc_feature    918..920
                     /label=stop
     misc_feature    915..917
                     /label=stop
     CDS             81..920
                     /label=BBa_K143032
     stem_loop       940..983
                     /label=stem_loop
     misc_feature    929..1008
                     /label=BBa_B0010
     terminator      929..1008
                     /label=BBa_B0010
     stem_loop       1024..1043
                     /label=T7 TE
     misc_feature    1050..1050
                     /label=stop
     polyA_site      1044..1057
                     /label=polya
     misc_feature    1017..1057
                     /label=BBa_B0012
     terminator      1017..1057
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaactcag gaccgaaagt ttctgtcatt atgggcattt
      121 ataattgcga acgcactttg gcagaaagca tagaatccat actcagccaa tcctataaaa
      181 attgggagct gattttgtgc gatgatgcgt caacagacgg cacgctccgt atcgcgaagc
      241 agtatgccgc tcattacagc gaccgcatca aactgattca aaacaaaaca aataagcggc
      301 ttgccgcatc attaaatcat tgtctttcgc atgcgacagg cgattatatc gaacgtcagg
      361 acggagatga cctttcgttt ccgcgccgtc tggaaaagca ggtcgcgttt ttagaaaagc
      421 accgacacta tcaggtggtt ggcaccggca tgcttgtgtt tgatgaattt ggcgtaagag
      481 gcgcccgcat tctgccttct gttccggagc cgggcatcat ggcaaaaggg actccatttt
      541 gccacggcac gattatgatg agagcgagtg cctaccgcac gctgaaaggc taccggtcgg
      601 tgcggcggac gagacgaatg gaagatattg atttgtggct tcgctttttt gaagagggct
      661 tcaggggcta taatcttcag gaagccttgt ataaagtgag ggaagacagc gatgcattca
      721 aacggcggtc atttacgtat tcaatcgaca atgccattct tgtctatcag gcgtgcagac
      781 gcttgaagct tcctttatct gattacatat atatcgcaaa accgttaatt cgcgccttta
      841 tgccggcagc tgtgatgaat cgctaccata aaaaaagagt gatgaaccaa aaggaagggc
      901 ttgtcaagca tgaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      961 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1021 actggctcac cttcgggtgg gcctttctgc gtttata
//