LOCUS BBa_K204020 940 bp DNA linear 22-Dec-2024 DEFINITION ptetR+YFP ACCESSION BBa_K204020 VERSION BBa_K204020.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..803 /label=BBa_E0030 stem_loop 823..866 /label=stem_loop misc_feature 812..891 /label=BBa_B0010 terminator 812..891 /label=BBa_B0010 stem_loop 907..926 /label=T7 TE misc_feature 933..933 /label=stop polyA_site 927..940 /label=polya misc_feature 900..940 /label=BBa_B0012 terminator 900..940 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc 121 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 241 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc 301 gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc gaaggctacg 361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 481 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 541 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 661 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg 721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 781 tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag 841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 901 cacactggct caccttcggg tgggcctttc tgcgtttata //