LOCUS       BBa_K204034             1945 bp    DNA     linear       22-Dec-2024
DEFINITION  3O<sub>6</sub>HSL receiver +GFP
ACCESSION   BBa_K204034
VERSION     BBa_K204034.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     RBS             1070..1082
                     /label=RBS-3\Weak
     RBS             1076..1079
                     /label=RBS
     misc_feature    1070..1082
                     /label=BBa_B0032
     RBS             1070..1082
                     /label=BBa_B0032
     CDS             1089..1808
                     /label=GFP protein
     CDS             1089..1808
                     /label=BBa_E0040
     stem_loop       1828..1871
                     /label=stem_loop
     misc_feature    1817..1896
                     /label=BBa_B0010
     terminator      1817..1896
                     /label=BBa_B0010
     stem_loop       1912..1931
                     /label=T7 TE
     misc_feature    1938..1938
                     /label=stop
     polyA_site      1932..1945
                     /label=polya
     misc_feature    1905..1945
                     /label=BBa_B0012
     terminator      1905..1945
                     /label=BBa_B0012
     misc_feature    1070..1945
                     /label=BBa_E0240
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagt cacacaggaa
     1081 agtactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1141 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1201 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1261 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
     1321 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     1381 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     1441 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     1501 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     1561 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     1621 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1681 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1741 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     1801 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1861 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1921 tcgggtgggc ctttctgcgt ttata
//