LOCUS       BBa_K204036             2094 bp    DNA     linear       22-Dec-2024
DEFINITION  pBAD + GFP
ACCESSION   BBa_K204036
VERSION     BBa_K204036.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1231
                     /label=RBS-3\Weak
     RBS             1225..1228
                     /label=RBS
     misc_feature    1219..1231
                     /label=BBa_B0032
     RBS             1219..1231
                     /label=BBa_B0032
     CDS             1238..1957
                     /label=GFP protein
     CDS             1238..1957
                     /label=BBa_E0040
     stem_loop       1977..2020
                     /label=stem_loop
     misc_feature    1966..2045
                     /label=BBa_B0010
     terminator      1966..2045
                     /label=BBa_B0010
     stem_loop       2061..2080
                     /label=T7 TE
     misc_feature    2087..2087
                     /label=stop
     polyA_site      2081..2094
                     /label=polya
     misc_feature    2054..2094
                     /label=BBa_B0012
     terminator      2054..2094
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagtc acacaggaaa gtactagatg cgtaaaggag aagaactttt
     1261 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
     1321 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
     1381 cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt
     1441 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat
     1501 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
     1561 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
     1621 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
     1681 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
     1741 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
     1801 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
     1861 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
     1921 gattacacat ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa
     1981 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2041 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//