LOCUS BBa_K204044 1077 bp DNA linear 25-Nov-2024 DEFINITION placI + RFP ACCESSION BBa_K204044 VERSION BBa_K204044.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..937 /label=mCherry misc_feature 578..578 /label=C->T (removing PstI site) misc_feature 227..940 /label=BBa_J06504 stem_loop 960..1003 /label=stem_loop misc_feature 949..1028 /label=BBa_B0010 terminator 949..1028 /label=BBa_B0010 stem_loop 1044..1063 /label=T7 TE misc_feature 1070..1070 /label=stop polyA_site 1064..1077 /label=polya misc_feature 1037..1077 /label=BBa_B0012 terminator 1037..1077 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg tgagcaaggg 241 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 301 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 361 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 421 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 481 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 541 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta 601 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 661 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 721 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 781 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 841 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 901 ccactccacc ggcggcatgg acgagctgta caagtaataa tactagagcc aggcatcaaa 961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //