LOCUS       BBa_K204048             1132 bp    DNA     linear       26-Dec-2024
DEFINITION  placI + mOrange
ACCESSION   BBa_K204048
VERSION     BBa_K204048.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..241
                     /label=MATSG linker
     misc_feature    950..964
                     /label=GSGTA linker
     CDS             227..964
                     /label=mOrange
     misc_feature    971..995
                     /label=Help:Barcodes
     CDS             227..995
                     /label=BBa_E2050
     stem_loop       1015..1058
                     /label=stem_loop
     misc_feature    1004..1083
                     /label=BBa_B0010
     terminator      1004..1083
                     /label=BBa_B0010
     stem_loop       1099..1118
                     /label=T7 TE
     misc_feature    1125..1125
                     /label=stop
     polyA_site      1119..1132
                     /label=polya
     misc_feature    1092..1132
                     /label=BBa_B0012
     terminator      1092..1132
                     /label=BBa_B0012
     terminator      1004..1132
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg caactagcgg
      241 catggttagt aaaggagaag aaaacaatat ggcaatcata aaagaattta tgagattcaa
      301 agtcagaatg gaaggttctg taaatggtca cgagttcgaa atagaagggg aaggtgaagg
      361 tagaccctat gaaggctttc aaacggctaa attaaaagtt accaagggtg gaccattgcc
      421 ctttgcttgg gatatcctgt ctcctcagtt cacttatggt agtaaagcct atgttaagca
      481 tcctgctgat attcctgatt acttcaagtt gagttttcca gaaggtttca aatgggagag
      541 agttatgaat tttgaagatg gcggagttgt gacagtgaca caagactcct cacttcaaga
      601 cggtgagttt atttacaagg taaaactacg tggcactaac tttccgtcgg atggaccagt
      661 catgcaaaaa aagacgatgg gttgggaggc ttcatctgag cgaatgtatc cagaagatgg
      721 ggcactaaag ggcgaaatta agatgaggct caaattaaag gatggtggac attatacctc
      781 ggaagtgaaa actacctata aagccaaaaa gccagttcaa ttacctggtg catacattgt
      841 tggcattaag ttggacatca caagccacaa tgaagattat acaatagtag agcagtacga
      901 acgcgcggaa ggtaggcatt ctactggagg catggatgaa ctatacaaag gttctggtac
      961 cgcataataa ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa
     1021 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1081 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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