LOCUS BBa_K204048 1132 bp DNA linear 14-Nov-2024 DEFINITION placI + mOrange ACCESSION BBa_K204048 VERSION BBa_K204048.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 227..241 /label=MATSG linker misc_feature 950..964 /label=GSGTA linker CDS 227..964 /label=mOrange misc_feature 971..995 /label=Help:Barcodes CDS 227..995 /label=BBa_E2050 stem_loop 1015..1058 /label=stem_loop misc_feature 1004..1083 /label=BBa_B0010 terminator 1004..1083 /label=BBa_B0010 stem_loop 1099..1118 /label=T7 TE misc_feature 1125..1125 /label=stop polyA_site 1119..1132 /label=polya misc_feature 1092..1132 /label=BBa_B0012 terminator 1092..1132 /label=BBa_B0012 terminator 1004..1132 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg caactagcgg 241 catggttagt aaaggagaag aaaacaatat ggcaatcata aaagaattta tgagattcaa 301 agtcagaatg gaaggttctg taaatggtca cgagttcgaa atagaagggg aaggtgaagg 361 tagaccctat gaaggctttc aaacggctaa attaaaagtt accaagggtg gaccattgcc 421 ctttgcttgg gatatcctgt ctcctcagtt cacttatggt agtaaagcct atgttaagca 481 tcctgctgat attcctgatt acttcaagtt gagttttcca gaaggtttca aatgggagag 541 agttatgaat tttgaagatg gcggagttgt gacagtgaca caagactcct cacttcaaga 601 cggtgagttt atttacaagg taaaactacg tggcactaac tttccgtcgg atggaccagt 661 catgcaaaaa aagacgatgg gttgggaggc ttcatctgag cgaatgtatc cagaagatgg 721 ggcactaaag ggcgaaatta agatgaggct caaattaaag gatggtggac attatacctc 781 ggaagtgaaa actacctata aagccaaaaa gccagttcaa ttacctggtg catacattgt 841 tggcattaag ttggacatca caagccacaa tgaagattat acaatagtag agcagtacga 901 acgcgcggaa ggtaggcatt ctactggagg catggatgaa ctatacaaag gttctggtac 961 cgcataataa ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa 1021 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 1081 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //