LOCUS       BBa_K204052              986 bp    DNA     linear       16-Dec-2024
DEFINITION  ptetR + mOrange
ACCESSION   BBa_K204052
VERSION     BBa_K204052.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..95
                     /label=MATSG linker
     misc_feature    804..818
                     /label=GSGTA linker
     CDS             81..818
                     /label=mOrange
     misc_feature    825..849
                     /label=Help:Barcodes
     CDS             81..849
                     /label=BBa_E2050
     stem_loop       869..912
                     /label=stem_loop
     misc_feature    858..937
                     /label=BBa_B0010
     terminator      858..937
                     /label=BBa_B0010
     stem_loop       953..972
                     /label=T7 TE
     misc_feature    979..979
                     /label=stop
     polyA_site      973..986
                     /label=polya
     misc_feature    946..986
                     /label=BBa_B0012
     terminator      946..986
                     /label=BBa_B0012
     terminator      858..986
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcaacta gcggcatggt tagtaaagga gaagaaaaca
      121 atatggcaat cataaaagaa tttatgagat tcaaagtcag aatggaaggt tctgtaaatg
      181 gtcacgagtt cgaaatagaa ggggaaggtg aaggtagacc ctatgaaggc tttcaaacgg
      241 ctaaattaaa agttaccaag ggtggaccat tgccctttgc ttgggatatc ctgtctcctc
      301 agttcactta tggtagtaaa gcctatgtta agcatcctgc tgatattcct gattacttca
      361 agttgagttt tccagaaggt ttcaaatggg agagagttat gaattttgaa gatggcggag
      421 ttgtgacagt gacacaagac tcctcacttc aagacggtga gtttatttac aaggtaaaac
      481 tacgtggcac taactttccg tcggatggac cagtcatgca aaaaaagacg atgggttggg
      541 aggcttcatc tgagcgaatg tatccagaag atggggcact aaagggcgaa attaagatga
      601 ggctcaaatt aaaggatggt ggacattata cctcggaagt gaaaactacc tataaagcca
      661 aaaagccagt tcaattacct ggtgcataca ttgttggcat taagttggac atcacaagcc
      721 acaatgaaga ttatacaata gtagagcagt acgaacgcgc ggaaggtagg cattctactg
      781 gaggcatgga tgaactatac aaaggttctg gtaccgcata ataactctga tagtgctagt
      841 gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      901 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      961 ttcgggtggg cctttctgcg tttata
//