LOCUS       BBa_K204062              981 bp    DNA     linear       26-Dec-2024
DEFINITION  pcI + mOrange
ACCESSION   BBa_K204062
VERSION     BBa_K204062.1  
FEATURES             Location/Qualifiers
     promoter        15..20
                     /label=-35
     promoter        25..41
                     /label=OR1
     promoter        1..17
                     /label=OR2
     promoter        38..43
                     /label=-10
     misc_feature    1..49
                     /label=BBa_R1051
     promoter        1..49
                     /label=BBa_R1051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     misc_feature    76..90
                     /label=MATSG linker
     misc_feature    799..813
                     /label=GSGTA linker
     CDS             76..813
                     /label=mOrange
     misc_feature    820..844
                     /label=Help:Barcodes
     CDS             76..844
                     /label=BBa_E2050
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
     terminator      853..981
                     /label=BBa_B0015
     misc_feature    58..981
                     /label=BBa_K204033
ORIGIN
        1 taacaccgtg cgtgttgaca attttacctc tggcggtgat actggttgct actagagaaa
       61 gaggagaaat actagatggc aactagcggc atggttagta aaggagaaga aaacaatatg
      121 gcaatcataa aagaatttat gagattcaaa gtcagaatgg aaggttctgt aaatggtcac
      181 gagttcgaaa tagaagggga aggtgaaggt agaccctatg aaggctttca aacggctaaa
      241 ttaaaagtta ccaagggtgg accattgccc tttgcttggg atatcctgtc tcctcagttc
      301 acttatggta gtaaagccta tgttaagcat cctgctgata ttcctgatta cttcaagttg
      361 agttttccag aaggtttcaa atgggagaga gttatgaatt ttgaagatgg cggagttgtg
      421 acagtgacac aagactcctc acttcaagac ggtgagttta tttacaaggt aaaactacgt
      481 ggcactaact ttccgtcgga tggaccagtc atgcaaaaaa agacgatggg ttgggaggct
      541 tcatctgagc gaatgtatcc agaagatggg gcactaaagg gcgaaattaa gatgaggctc
      601 aaattaaagg atggtggaca ttatacctcg gaagtgaaaa ctacctataa agccaaaaag
      661 ccagttcaat tacctggtgc atacattgtt ggcattaagt tggacatcac aagccacaat
      721 gaagattata caatagtaga gcagtacgaa cgcgcggaag gtaggcattc tactggaggc
      781 atggatgaac tatacaaagg ttctggtacc gcataataac tctgatagtg ctagtgtaga
      841 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat a
//