BBa_K204062
type: ComponentDefinition
persistent identity: BBa K204062
display id: BBa_K204062
version: 1
was generated by: iGEM to SBOL conversion
description: pcI + mOrange
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: pcI + mOrange
owned by: james
owned by: myers
mutable description: pcI + mOrange
discontinued: false
dominant: false
group u list: _304_
m user id: 0
owner id: 5360
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: pcI + mOrange
star: false
creator: Uno Keisuke
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R1051
component: BBa_K204033
sequence annotation: BBa_R1051
sequence annotation: BBa_K204033
sequence: https://synbiohub.org/public/igem/BBa_K204062_sequence/1

BBa_R1051
type: Component
persistent identity: component2256154
display id: component2256154
version: 1
top level: BBa_K204062
definition: cI lam
access: public

BBa_K204033
type: Component
persistent identity: component2256172
display id: component2256172
version: 1
top level: BBa_K204062
definition: BBa_K204033
access: public

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation2256154
display id: annotation2256154
version: 1
top level: BBa_K204062
location: https://synbiohub.org/public/igem/BBa_K204062/annotation2256154/range2256154/1
component: BBa_R1051

https://synbiohub.org/public/igem/BBa_K204062/annotation2256154/range2256154/1
type: Range
persistent identity: range2256154
display id: range2256154
version: 1
top level: BBa_K204062
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_K204033
type: SequenceAnnotation
persistent identity: annotation2256172
display id: annotation2256172
version: 1
top level: BBa_K204062
location: https://synbiohub.org/public/igem/BBa_K204062/annotation2256172/range2256172/1
component: BBa_K204033

https://synbiohub.org/public/igem/BBa_K204062/annotation2256172/range2256172/1
type: Range
persistent identity: range2256172
display id: range2256172
version: 1
top level: BBa_K204062
direction: direction/reverse
start: 58
end: 981
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R1051
display id: BBa_R1051
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter, Standard (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: myers
owned by: james
part status: Released HQ 2013
mutable description: [Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Modified to comply with BBa_S0001: TTGACA-17N-GATACT Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross Drew Endy
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R1051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1051_sequence/1

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation7074
display id: annotation7074
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1
type: Range
persistent identity: range7074
display id: range7074
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2077
display id: annotation2077
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1
type: Range
persistent identity: range2077
display id: range2077
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2075
display id: annotation2075
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1
type: Range
persistent identity: range2075
display id: range2075
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2076
display id: annotation2076
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1
type: Range
persistent identity: range2076
display id: range2076
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2078
display id: annotation2078
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1
type: Range
persistent identity: range2078
display id: range2078
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_K204033
type: ComponentDefinition
persistent identity: BBa K204033
display id: BBa_K204033
version: 1
was generated by: iGEM to SBOL conversion
description: mOrange
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: RBS+mOrange+terminater
owned by: james
owned by: myers
mutable description: mOrange RBS+mOrange+terminater
discontinued: false
dominant: false
group u list: _304_
m user id: 0
owner id: 5360
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: RBS+mOrange+terminater
star: false
creator: Uno Keisuke
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E2050
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_E2050
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K204033_sequence/1

BBa_E2050
type: Component
persistent identity: component2249853
display id: component2249853
version: 1
top level: BBa_K204033
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component2249848
display id: component2249848
version: 1
top level: BBa_K204033
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2249860
display id: component2249860
version: 1
top level: BBa_K204033
definition: BBa_B0015
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249848
display id: annotation2249848
version: 1
top level: BBa_K204033
location: https://synbiohub.org/public/igem/BBa_K204033/annotation2249848/range2249848/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K204033/annotation2249848/range2249848/1
type: Range
persistent identity: range2249848
display id: range2249848
version: 1
top level: BBa_K204033
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E2050
type: SequenceAnnotation
persistent identity: annotation2249853
display id: annotation2249853
version: 1
top level: BBa_K204033
location: https://synbiohub.org/public/igem/BBa_K204033/annotation2249853/range2249853/1
component: BBa_E2050

https://synbiohub.org/public/igem/BBa_K204033/annotation2249853/range2249853/1
type: Range
persistent identity: range2249853
display id: range2249853
version: 1
top level: BBa_K204033
direction: direction/reverse
start: 19
end: 787
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249860
display id: annotation2249860
version: 1
top level: BBa_K204033
location: https://synbiohub.org/public/igem/BBa_K204033/annotation2249860/range2249860/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K204033/annotation2249860/range2249860/1
type: Range
persistent identity: range2249860
display id: range2249860
version: 1
top level: BBa_K204033
direction: direction/reverse
start: 796
end: 924
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E2050
display id: BBa_E2050
version: 1
was generated by: iGEM to SBOL conversion
description: derivative of mRFP1, yeast-optimized
created: 2005-01-24T12:00:00Z
modified: 2016-01-25T02:35:54Z
mutable provenance: Shaner et al, 2004. Nat Biotech (22):1567-1571
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP derivative. Ex548nm/Em562. yeast codon optimized.
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 4206
owner id: 230
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mCherry (BBa_E2060).
star: true
creator: ryu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MATSG linker
sequence annotation: mOrange
sequence annotation: GSGTA linker
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E2050_sequence/1

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431915
display id: annotation1431915
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431915/range1431915/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_E2050/annotation1431915/range1431915/1
type: Range
persistent identity: range1431915
display id: range1431915
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 15
orientation: inline

mOrange
type: SequenceAnnotation
persistent identity: annotation1431920
display id: annotation1431920
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431920/range1431920/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E2050/annotation1431920/range1431920/1
type: Range
persistent identity: range1431920
display id: range1431920
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 738
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431919
display id: annotation1431919
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431919/range1431919/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_E2050/annotation1431919/range1431919/1
type: Range
persistent identity: range1431919
display id: range1431919
version: 1
top level: mRFP1
direction: direction/forward
start: 724
end: 738
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214024
display id: annotation2214024
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E2050/annotation2214024/range2214024/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_E2050/annotation2214024/range2214024/1
type: Range
persistent identity: range2214024
display id: range2214024
version: 1
top level: mRFP1
direction: direction/forward
start: 745
end: 769
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K204062_sequence/1
type: Sequence
persistent identity: BBa K204062 sequence
display id: BBa_K204062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgacaattttacctctggcggtgatactggttgctactagagaaagaggagaaatactagatggcaactagcggcatggttagtaaaggagaagaaaacaatatggcaatcataaaagaatttatgagattcaaagtcagaatggaaggttctgtaaatggtcacgagttcgaaatagaaggggaaggtgaaggtagaccctatgaaggctttcaaacggctaaattaaaagttaccaagggtggaccattgccctttgcttgggatatcctgtctcctcagttcacttatggtagtaaagcctatgttaagcatcctgctgatattcctgattacttcaagttgagttttccagaaggtttcaaatgggagagagttatgaattttgaagatggcggagttgtgacagtgacacaagactcctcacttcaagacggtgagtttatttacaaggtaaaactacgtggcactaactttccgtcggatggaccagtcatgcaaaaaaagacgatgggttgggaggcttcatctgagcgaatgtatccagaagatggggcactaaagggcgaaattaagatgaggctcaaattaaaggatggtggacattatacctcggaagtgaaaactacctataaagccaaaaagccagttcaattacctggtgcatacattgttggcattaagttggacatcacaagccacaatgaagattatacaatagtagagcagtacgaacgcgcggaaggtaggcattctactggaggcatggatgaactatacaaaggttctggtaccgcataataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R1051_sequence/1
type: Sequence
persistent identity: BBa R1051 sequence
display id: BBa_R1051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgacaattttacctctggcggtgatactggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K204033_sequence/1
type: Sequence
persistent identity: BBa K204033 sequence
display id: BBa_K204033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcaactagcggcatggttagtaaaggagaagaaaacaatatggcaatcataaaagaatttatgagattcaaagtcagaatggaaggttctgtaaatggtcacgagttcgaaatagaaggggaaggtgaaggtagaccctatgaaggctttcaaacggctaaattaaaagttaccaagggtggaccattgccctttgcttgggatatcctgtctcctcagttcacttatggtagtaaagcctatgttaagcatcctgctgatattcctgattacttcaagttgagttttccagaaggtttcaaatgggagagagttatgaattttgaagatggcggagttgtgacagtgacacaagactcctcacttcaagacggtgagtttatttacaaggtaaaactacgtggcactaactttccgtcggatggaccagtcatgcaaaaaaagacgatgggttgggaggcttcatctgagcgaatgtatccagaagatggggcactaaagggcgaaattaagatgaggctcaaattaaaggatggtggacattatacctcggaagtgaaaactacctataaagccaaaaagccagttcaattacctggtgcatacattgttggcattaagttggacatcacaagccacaatgaagattatacaatagtagagcagtacgaacgcgcggaaggtaggcattctactggaggcatggatgaactatacaaaggttctggtaccgcataataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E2050_sequence/1
type: Sequence
persistent identity: BBa E2050 sequence
display id: BBa_E2050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaactagcggcatggttagtaaaggagaagaaaacaatatggcaatcataaaagaatttatgagattcaaagtcagaatggaaggttctgtaaatggtcacgagttcgaaatagaaggggaaggtgaaggtagaccctatgaaggctttcaaacggctaaattaaaagttaccaagggtggaccattgccctttgcttgggatatcctgtctcctcagttcacttatggtagtaaagcctatgttaagcatcctgctgatattcctgattacttcaagttgagttttccagaaggtttcaaatgggagagagttatgaattttgaagatggcggagttgtgacagtgacacaagactcctcacttcaagacggtgagtttatttacaaggtaaaactacgtggcactaactttccgtcggatggaccagtcatgcaaaaaaagacgatgggttgggaggcttcatctgagcgaatgtatccagaagatggggcactaaagggcgaaattaagatgaggctcaaattaaaggatggtggacattatacctcggaagtgaaaactacctataaagccaaaaagccagttcaattacctggtgcatacattgttggcattaagttggacatcacaagccacaatgaagattatacaatagtagagcagtacgaacgcgcggaaggtaggcattctactggaggcatggatgaactatacaaaggttctggtaccgcataataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z