LOCUS BBa_K204086 2876 bp DNA linear 22-Dec-2024 DEFINITION 3O6HSL receiver + RFP + cI represser ACCESSION BBa_K204086 VERSION BBa_K204086.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_K204051 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 CDS 1088..1798 /label=mCherry misc_feature 1439..1439 /label=C->T (removing PstI site) misc_feature 1088..1801 /label=BBa_J06504 stem_loop 1821..1864 /label=stem_loop misc_feature 1810..1889 /label=BBa_B0010 terminator 1810..1889 /label=BBa_B0010 stem_loop 1905..1924 /label=T7 TE misc_feature 1931..1931 /label=stop polyA_site 1925..1938 /label=polya misc_feature 1898..1938 /label=BBa_B0012 terminator 1898..1938 /label=BBa_B0012 misc_feature 1070..1938 /label=BBa_J06702 misc_feature 1951..1954 /label=conserved RBS 1947..1958 /label=BBa_B0034 CDS 1968..2675 /label=cI lambda misc_feature 2676..2708 /label=LVA misc_feature 2715..2739 /label=Help:Barcodes CDS 1965..2739 /label=BBa_C0051 stem_loop 2759..2802 /label=stem_loop misc_feature 2748..2827 /label=BBa_B0010 terminator 2748..2827 /label=BBa_B0010 stem_loop 2843..2862 /label=T7 TE misc_feature 2869..2869 /label=stop polyA_site 2863..2876 /label=polya misc_feature 2836..2876 /label=BBa_B0012 terminator 2836..2876 /label=BBa_B0012 terminator 2748..2876 /label=BBa_B0015 misc_feature 1947..2876 /label=BBa_K204069 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg 1141 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg 1201 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc 1261 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt 1321 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg 1381 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctcctt 1441 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg 1501 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga 1561 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta 1621 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta 1681 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca 1741 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaata 1801 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 1861 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 1921 ggcctttctg cgtttatata ctagagaaag aggagaaata ctagatgagc acaaaaaaga 1981 aaccattaac acaagagcag cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa 2041 agaaaaatga acttggctta tcccaggaat ctgtcgcaga caagatgggg atggggcagt 2101 caggcgttgg tgctttattt aatggcatca atgcattaaa tgcttataac gccgcattgc 2161 ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc agagaaatct 2221 acgagatgta tgaagcggtt agtatgcagc cgtcacttag aagtgagtat gagtaccctg 2281 ttttttctca tgttcaggca gggatgttct cacctgagct tagaaccttt accaaaggtg 2341 atgcggagag atgggtaagc acaaccaaaa aagccagtga ttctgcattc tggcttgagg 2401 ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc aagctttcct gacggaatgt 2461 taattctcgt tgaccctgag caggctgttg agccaggtga tttctgcata gccagacttg 2521 ggggtgatga gtttaccttc aagaaactga tcagggatag cggtcaggtg tttttacaac 2581 cactaaaccc acagtaccca atgatcccat gcaatgagag ttgttccgtt gtggggaaag 2641 ttatcgctag tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa aactacgctt 2701 tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat 2761 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2821 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //