LOCUS BBa_K204091 4773 bp DNA linear 15-Nov-2024 DEFINITION plac+crtE+crtB+crtI+crtY ACCESSION BBa_K204091 VERSION BBa_K204091.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..212 /label=BBa_K118012 CDS 219..1130 /label=BBa_I742151 misc_feature 1139..1141 /label=SacI RBS 1142..1148 /label=rbs RBS 1139..1148 /label=BBa_J15001 CDS 1155..2084 /label=crtB coding sequence CDS 1155..2087 /label=BBa_K118002 misc_feature 2100..2103 /label=conserved RBS 2096..2107 /label=BBa_B0034 misc_feature 2591..2596 /label=PstI site removed misc_feature 3002..3007 /label=PstI site removed CDS 2114..3592 /label=crtI coding sequence CDS 2114..3595 /label=BBa_K118003 misc_feature 3608..3611 /label=conserved RBS 3604..3615 /label=BBa_B0034 CDS 3622..4770 /label=crtY coding sequence CDS 3622..4773 /label=BBa_K118008 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagga ggtactagat gacggtctgc gcaaaaaaac 241 acgttcatct cactcgcgat gctgcggagc agttactggc tgatattgat cgacgccttg 301 atcagttatt gcccgtggag ggagaacggg atgttgtggg tgccgcgatg cgtgaaggtg 361 cgctggcacc gggaaaacgt attcgcccca tgttgctgtt gctgaccgcc cgcgatctgg 421 gttgcgctgt cagccatgac ggattactgg atttggcctg tgcggtggaa atggtccacg 481 cggcttcgct gatccttgac gatatgccct gcatggacga tgcgaagctg cggcgcggac 541 gccctaccat tcattctcat tacggagagc atgtggcaat actggcggcg gttgccttgc 601 tgagtaaagc ctttggcgta attgccgatg cagatggcct cacgccgctg gcaaaaaatc 661 gggcggtttc tgaactgtca aacgccatcg gcatgcaagg attggttcag ggtcagttca 721 aggatctgtc tgaaggggat aagccgcgca gcgctgaagc tattttgatg acgaatcact 781 ttaaaaccag cacgctgttt tgtgcctcca tgcagatggc ctcgattgtt gcgaatgcct 841 ccagcgaagc gcgtgattgc ctgcatcgtt tttcacttga tcttggtcag gcatttcaac 901 tgctggacga tttgaccgat ggcatgaccg acaccggtaa ggatagcaat caggacgccg 961 gtaaatcgac gctggtcaat ctgttaggcc cgagggcggt tgaagaacgt ctgagacaac 1021 atcttcagct tgccagtgag catctctctg cggcctgcca acacgggcac gccactcaac 1081 attttattca ggcctggttt gacaaaaaac tcgctgccgt cagttaataa tactagagct 1141 caaggaggta ctagatgaat aatccgtcgt tactcaatca tgcggtcgaa acgatggcag 1201 ttggctcgaa aagttttgcg acagcctcaa agttatttga tgcaaaaacc cggcgcagcg 1261 tactgatgct ctacgcctgg tgccgccatt gtgacgatgt tattgacgat cagacgctgg 1321 gctttcaggc ccggcagcct gccttacaaa cgcccgaaca acgtctgatg caacttgaga 1381 tgaaaacgcg ccaggcctat gcaggatcgc agatgcacga accggcgttt gcggcttttc 1441 aggaagtggc tatggctcat gatatcgccc cggcttacgc gtttgatcat ctggaaggct 1501 tcgccatgga tgtacgcgaa gcgcaataca gccaactgga tgatacgctg cgctattgct 1561 atcacgttgc aggcgttgtc ggcttgatga tggcgcaaat catgggcgtg cgggataacg 1621 ccacgctgga ccgcgcctgt gaccttgggc tggcatttca gttgaccaat attgctcgcg 1681 atattgtgga cgatgcgcat gcgggccgct gttatctgcc ggcaagctgg ctggagcatg 1741 aaggtctgaa caaagagaat tatgcggcac ctgaaaaccg tcaggcgctg agccgtatcg 1801 cccgtcgttt ggtgcaggaa gcagaacctt actatttgtc tgccacagcc ggcctggcag 1861 ggttgcccct gcgttccgcc tgggcaatcg ctacggcgaa gcaggtttac cggaaaatag 1921 gtgtcaaagt tgaacaggcc ggtcagcaag cctgggatca gcggcagtca acgaccacgc 1981 ccgaaaaatt aacgctgctg ctggccgcct ctggtcaggc ccttacttcc cggatgcggg 2041 ctcatcctcc ccgccctgcg catctctggc agcgcccgct ctaataatac tagagaaaga 2101 ggagaaatac tagatgaaac caactacggt aattggtgca ggcttcggtg gcctggcact 2161 ggcaattcgt ctacaagctg cggggatccc cgtcttactg cttgaacaac gtgataaacc 2221 cggcggtcgg gcttatgtct acgaggatca ggggtttacc tttgatgcag gcccgacggt 2281 tatcaccgat cccagtgcca ttgaagaact gtttgcactg gcaggaaaac agttaaaaga 2341 gtatgtcgaa ctgctgccgg ttacgccgtt ttaccgcctg tgttgggagt cagggaaggt 2401 ctttaattac gataacgatc aaacccggct cgaagcgcag attcagcagt ttaatccccg 2461 cgatgtcgaa ggttatcgtc agtttctgga ctattcacgc gcggtgttta aagaaggcta 2521 tctaaagctc ggtactgtcc cttttttatc gttcagagac atgcttcgcg ccgcacctca 2581 actggcgaaa ctgcaagcat ggagaagcgt ttacagtaag gttgccagtt acatcgaaga 2641 tgaacatctg cgccaggcgt tttctttcca ctcgctgttg gtgggcggca atcccttcgc 2701 cacctcatcc atttatacgt tgatacacgc gctggagcgt gagtggggcg tctggtttcc 2761 gcgtggcggc accggcgcat tagttcaggg gatgataaag ctgtttcagg atctgggtgg 2821 cgaagtcgtg ttaaacgcca gagtcagcca tatggaaacg acaggaaaca agattgaagc 2881 cgtgcattta gaggacggtc gcaggttcct gacgcaagcc gtcgcgtcaa atgcagatgt 2941 ggttcatacc tatcgcgacc tgttaagcca gcaccctgcc gcggttaagc agtccaacaa 3001 actgcaaact aagcgcatga gtaactctct gtttgtgctc tattttggtt tgaatcacca 3061 tcatgatcag ctcgcgcatc acacggtttg tttcggcccg cgttaccgcg agctgattga 3121 cgaaattttt aatcatgatg gcctcgcaga ggacttctca ctttatctgc acgcgccctg 3181 tgtcacggat tcgtcactgg cgcctgaagg ttgcggcagt tactatgtgt tggcgccggt 3241 gccgcattta ggcaccgcga acctcgactg gacggttgag gggccaaaac tacgcgaccg 3301 tatttttgcg taccttgagc agcattacat gcctggctta cggagtcagc tggtcacgca 3361 ccggatgttt acgccgtttg attttcgcga ccagcttaat gcctatcatg gctcagcctt 3421 ttctgtggag cccgttctta cccagagcgc ctggtttcgg ccgcataacc gcgataaaac 3481 cattactaat ctctacctgg tcggcgcagg cacgcatccc ggcgcaggca ttcctggcgt 3541 catcggctcg gcaaaagcga cagcaggttt gatgctggag gatctgatat aataatacta 3601 gagaaagagg agaaatacta gatgcaaccg cattatgatc tgattctcgt gggggctgga 3661 ctcgcgaatg gccttatcgc cctgcgtctt cagcagcagc aacctgatat gcgtattttg 3721 cttatcgacg ccgcacccca ggcgggcggg aatcatacgt ggtcatttca ccacgatgat 3781 ttgactgaga gccaacatcg ttggatagct ccgctggtgg ttcatcactg gcccgactat 3841 caggtacgct ttcccacacg ccgtcgtaag ctgaacagcg gctacttttg tattacttct 3901 cagcgtttcg ctgaggtttt acagcgacag tttggcccgc acttgtggat ggataccgcg 3961 gtcgcagagg ttaatgcgga atctgttcgg ttgaaaaagg gtcaggttat cggtgcccgc 4021 gcggtgattg acgggcgggg ttatgcggca aattcagcac tgagcgtggg cttccaggcg 4081 tttattggcc aggaatggcg attgagccac ccgcatggtt tatcgtctcc cattatcatg 4141 gatgccacgg tcgatcagca aaatggttat cgcttcgtgt acagcctgcc gctctcgccg 4201 accagattgt taattgaaga cacgcactat attgataatg cgacattaga tcctgaatgc 4261 gcgcggcaaa atatttgcga ctatgccgcg caacagggtt ggcagcttca gacactgctg 4321 cgagaagaac agggcgcctt acccattact ctgtcgggca atgccgacgc attctggcag 4381 cagcgccccc tggcctgtag tggattacgt gccggtctgt tccatcctac caccggctat 4441 tcactgccgc tggcggttgc cgtggccgac cgcctgagtg cacttgatgt ctttacgtcg 4501 gcctcaattc accatgccat tacgcatttt gcccgcgagc gctggcagca gcagggcttt 4561 ttccgcatgc tgaatcgcat gctgttttta gccggacccg ccgattcacg ctggcgggtt 4621 atgcagcgtt tttatggttt acctgaagat ttaattgccc gtttttatgc gggaaaactc 4681 acgctgaccg atcggctacg tattctgagc ggcaagccgc ctgttccggt attagcagca 4741 ttgcaagcca ttatgacgac tcatcgttaa taa //