BBa_K204091
type: ComponentDefinition
persistent identity: BBa K204091
display id: BBa_K204091
version: 1
was generated by: iGEM to SBOL conversion
description: plac+crtE+crtB+crtI+crtY
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: plac+crtE+crtB+crtI+crtY
owned by: james
owned by: myers
mutable description: plac+crtE+crtB+crtI+crtY
discontinued: false
dominant: false
group u list: _304_
m user id: 0
owner id: 5360
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: plac+crtE+crtB+crtI+crtY
star: false
creator: Uno Keisuke
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K118003
component: BBa_K118008
component: BBa_J15001
component: BBa_I742151
component: BBa_K118002
component: BBa_K118012
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_K118012
sequence annotation: BBa_I742151
sequence annotation: BBa_J15001
sequence annotation: BBa_K118002
sequence annotation: BBa_B0034
sequence annotation: BBa_K118003
sequence annotation: BBa_K118008
sequence: https://synbiohub.org/public/igem/BBa_K204091_sequence/1

BBa_B0034
type: Component
persistent identity: component2045711
display id: component2045711
version: 1
top level: BBa_K204091
definition: BBa_B0034
access: public

BBa_K118003
type: Component
persistent identity: component2045709
display id: component2045709
version: 1
top level: BBa_K204091
definition: BBa_K118003
access: public

BBa_K118008
type: Component
persistent identity: component2045713
display id: component2045713
version: 1
top level: BBa_K204091
definition: BBa_K118008
access: public

BBa_J15001
type: Component
persistent identity: component2045701
display id: component2045701
version: 1
top level: BBa_K204091
definition: BBa_J15001
access: public

BBa_I742151
type: Component
persistent identity: component2045698
display id: component2045698
version: 1
top level: BBa_K204091
definition: BBa_I742151
access: public

BBa_B0034
type: Component
persistent identity: component2045705
display id: component2045705
version: 1
top level: BBa_K204091
definition: BBa_B0034
access: public

BBa_K118002
type: Component
persistent identity: component2045703
display id: component2045703
version: 1
top level: BBa_K204091
definition: BBa_K118002
access: public

BBa_K118012
type: Component
persistent identity: component2045697
display id: component2045697
version: 1
top level: BBa_K204091
definition: BBa_K118012
access: public

BBa_R0010
type: Component
persistent identity: component2045690
display id: component2045690
version: 1
top level: BBa_K204091
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2045690
display id: annotation2045690
version: 1
top level: BBa_K204091
location: https://synbiohub.org/public/igem/BBa_K204091/annotation2045690/range2045690/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K204091/annotation2045690/range2045690/1
type: Range
persistent identity: range2045690
display id: range2045690
version: 1
top level: BBa_K204091
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2045697
display id: annotation2045697
version: 1
top level: BBa_K204091
location: https://synbiohub.org/public/igem/BBa_K204091/annotation2045697/range2045697/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K204091/annotation2045697/range2045697/1
type: Range
persistent identity: range2045697
display id: range2045697
version: 1
top level: BBa_K204091
direction: direction/reverse
start: 209
end: 212
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2045698
display id: annotation2045698
version: 1
top level: BBa_K204091
location: https://synbiohub.org/public/igem/BBa_K204091/annotation2045698/range2045698/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K204091/annotation2045698/range2045698/1
type: Range
persistent identity: range2045698
display id: range2045698
version: 1
top level: BBa_K204091
direction: direction/reverse
start: 219
end: 1130
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2045701
display id: annotation2045701
version: 1
top level: BBa_K204091
location: https://synbiohub.org/public/igem/BBa_K204091/annotation2045701/range2045701/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K204091/annotation2045701/range2045701/1
type: Range
persistent identity: range2045701
display id: range2045701
version: 1
top level: BBa_K204091
direction: direction/reverse
start: 1139
end: 1148
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2045703
display id: annotation2045703
version: 1
top level: BBa_K204091
location: https://synbiohub.org/public/igem/BBa_K204091/annotation2045703/range2045703/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K204091/annotation2045703/range2045703/1
type: Range
persistent identity: range2045703
display id: range2045703
version: 1
top level: BBa_K204091
direction: direction/reverse
start: 1155
end: 2087
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2045705
display id: annotation2045705
version: 1
top level: BBa_K204091
location: https://synbiohub.org/public/igem/BBa_K204091/annotation2045705/range2045705/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K204091/annotation2045705/range2045705/1
type: Range
persistent identity: range2045705
display id: range2045705
version: 1
top level: BBa_K204091
direction: direction/reverse
start: 2096
end: 2107
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2045709
display id: annotation2045709
version: 1
top level: BBa_K204091
location: https://synbiohub.org/public/igem/BBa_K204091/annotation2045709/range2045709/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K204091/annotation2045709/range2045709/1
type: Range
persistent identity: range2045709
display id: range2045709
version: 1
top level: BBa_K204091
direction: direction/reverse
start: 2114
end: 3595
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2045711
display id: annotation2045711
version: 1
top level: BBa_K204091
location: https://synbiohub.org/public/igem/BBa_K204091/annotation2045711/range2045711/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K204091/annotation2045711/range2045711/1
type: Range
persistent identity: range2045711
display id: range2045711
version: 1
top level: BBa_K204091
direction: direction/reverse
start: 3604
end: 3615
orientation: inline

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation2045713
display id: annotation2045713
version: 1
top level: BBa_K204091
location: https://synbiohub.org/public/igem/BBa_K204091/annotation2045713/range2045713/1
component: BBa_K118008

https://synbiohub.org/public/igem/BBa_K204091/annotation2045713/range2045713/1
type: Range
persistent identity: range2045713
display id: range2045713
version: 1
top level: BBa_K204091
direction: direction/reverse
start: 3622
end: 4773
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

BBa_I742151
type: ComponentDefinition
persistent identity: BBa I742151
display id: BBa_I742151
version: 1
was generated by: iGEM to SBOL conversion
description: crtE (geranylgeranyl pyrophosphate synthase) coding sequence.
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
mutable description: crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis parthway.
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No important considerations.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I742151_sequence/1

BBa_K118002
type: ComponentDefinition
persistent identity: BBa K118002
display id: BBa_K118002
version: 1
was generated by: iGEM to SBOL conversion
description: crtB coding sequence encoding phytoene synthase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtB coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118002_sequence/1

crtB coding sequence
type: SequenceAnnotation
persistent identity: annotation1995701
display id: annotation1995701
version: 1
top level: BBa_K118002
location: https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
type: Range
persistent identity: range1995701
display id: range1995701
version: 1
top level: BBa_K118002
direction: direction/forward
start: 1
end: 930
orientation: inline

BBa_K118003
type: ComponentDefinition
persistent identity: BBa K118003
display id: BBa_K118003
version: 1
was generated by: iGEM to SBOL conversion
description: crtI coding sequence encoding phytoene dehydrogenase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtI coding sequence
sequence annotation: PstI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118003_sequence/1

crtI coding sequence
type: SequenceAnnotation
persistent identity: annotation1995702
display id: annotation1995702
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
type: Range
persistent identity: range1995702
display id: range1995702
version: 1
top level: BBa_K118003
direction: direction/forward
start: 1
end: 1479
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968244
display id: annotation1968244
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
type: Range
persistent identity: range1968244
display id: range1968244
version: 1
top level: BBa_K118003
direction: direction/forward
start: 478
end: 483
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968245
display id: annotation1968245
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
type: Range
persistent identity: range1968245
display id: range1968245
version: 1
top level: BBa_K118003
direction: direction/forward
start: 889
end: 894
orientation: inline

BBa_K118008
type: ComponentDefinition
persistent identity: BBa K118008
display id: BBa_K118008
version: 1
was generated by: iGEM to SBOL conversion
description: crtY coding sequence encoding lycopene B-cyclase
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: true
creator: Andrew Hall
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: crtY coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118008_sequence/1

crtY coding sequence
type: SequenceAnnotation
persistent identity: annotation1995716
display id: annotation1995716
version: 1
top level: BBa_K118008
location: https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1
type: Range
persistent identity: range1995716
display id: range1995716
version: 1
top level: BBa_K118008
direction: direction/forward
start: 1
end: 1149
orientation: inline

BBa_K118012
type: ComponentDefinition
persistent identity: BBa K118012
display id: BBa_K118012
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic ribosome binding site added by Son of Babel procedure
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: This is a 4bp synthetic ribosome binding site which can be added by fusion PCR using a reverse primer and the Son of Babel cloning system.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The part can be added by fusion PCR using a Son of Babel reverse primer.
star: false
creator: Andrew Hall
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_K118012_sequence/1

https://synbiohub.org/public/igem/BBa_K204091_sequence/1
type: Sequence
persistent identity: BBa K204091 sequence
display id: BBa_K204091_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagaggaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742151_sequence/1
type: Sequence
persistent identity: BBa I742151 sequence
display id: BBa_I742151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118002_sequence/1
type: Sequence
persistent identity: BBa K118002 sequence
display id: BBa_K118002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118003_sequence/1
type: Sequence
persistent identity: BBa K118003 sequence
display id: BBa_K118003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118008_sequence/1
type: Sequence
persistent identity: BBa K118008 sequence
display id: BBa_K118008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118012_sequence/1
type: Sequence
persistent identity: BBa K118012 sequence
display id: BBa_K118012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: gagg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z