LOCUS       BBa_K2041005             775 bp    DNA     linear       23-May-2024
DEFINITION  ptet-RBS-fimE-T
ACCESSION   BBa_K2041005
VERSION     BBa_K2041005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..638
                     /label=BBa_K137007
     stem_loop       658..701
                     /label=stem_loop
     misc_feature    647..726
                     /label=BBa_B0010
     terminator      647..726
                     /label=BBa_B0010
     stem_loop       742..761
                     /label=T7 TE
     misc_feature    768..768
                     /label=stop
     polyA_site      762..775
                     /label=polya
     misc_feature    735..775
                     /label=BBa_B0012
     terminator      735..775
                     /label=BBa_B0012
     terminator      647..775
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatgcagg cggtttgtta cggggcaacg ggagccagag
      121 attattgtct tattctgttg gcatatcggc atgggatgcg tattagtgaa ctgcttgatc
      181 tgcattatca ggaccttgac cttaatgaag gtagaataaa tattcgccga ctgaagaacg
      241 gattttctac cgttcacccg ttacgttttg atgagcgtga agccgtggaa cgctggaccc
      301 aggaacgtgc taactggaaa ggcgctgacc ggactgacgc tatatttatt tctcgccgcg
      361 ggagtcggct ttctcgccag caggcctatc gcattattcg cgatgccggt attgaagctg
      421 gaaccgtaac gcagactcat cctcatatgt taaggcatgc ttgcggttat gaattggcgg
      481 agcgtggtgc agatactcgt ttaattcagg attatctcgg gcatcgaaat attcgccata
      541 ctgtgcgtta taccgccagt aatgctgctc gttttgccgg attatgggaa agaaataatc
      601 tcataaacga aaaattaaaa agagaagagg tttaataata ctagagccag gcatcaaata
      661 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      721 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//