LOCUS       BBa_K2041006            1466 bp    DNA     linear       18-May-2024
DEFINITION  ptet-RBS-tetR-fimE-T
ACCESSION   BBa_K2041006
VERSION     BBa_K2041006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             84..700
                     /label=tetR
     misc_feature    701..734
                     /label=SsrA
     misc_feature    741..765
                     /label=Help:Barcodes
     CDS             81..765
                     /label=BBa_C0040
     CDS             772..1329
                     /label=BBa_K137007
     stem_loop       1349..1392
                     /label=stem_loop
     misc_feature    1338..1417
                     /label=BBa_B0010
     terminator      1338..1417
                     /label=BBa_B0010
     stem_loop       1433..1452
                     /label=T7 TE
     misc_feature    1459..1459
                     /label=stop
     polyA_site      1453..1466
                     /label=polya
     misc_feature    1426..1466
                     /label=BBa_B0012
     terminator      1426..1466
                     /label=BBa_B0012
     terminator      1338..1466
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat
      121 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      181 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      241 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      301 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      361 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      421 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      481 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      541 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      601 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      661 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      721 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gatgatgcag
      781 gcggtttgtt acggggcaac gggagccaga gattattgtc ttattctgtt ggcatatcgg
      841 catgggatgc gtattagtga actgcttgat ctgcattatc aggaccttga ccttaatgaa
      901 ggtagaataa atattcgccg actgaagaac ggattttcta ccgttcaccc gttacgtttt
      961 gatgagcgtg aagccgtgga acgctggacc caggaacgtg ctaactggaa aggcgctgac
     1021 cggactgacg ctatatttat ttctcgccgc gggagtcggc tttctcgcca gcaggcctat
     1081 cgcattattc gcgatgccgg tattgaagct ggaaccgtaa cgcagactca tcctcatatg
     1141 ttaaggcatg cttgcggtta tgaattggcg gagcgtggtg cagatactcg tttaattcag
     1201 gattatctcg ggcatcgaaa tattcgccat actgtgcgtt ataccgccag taatgctgct
     1261 cgttttgccg gattatggga aagaaataat ctcataaacg aaaaattaaa aagagaagag
     1321 gtttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1381 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1441 ttcgggtggg cctttctgcg tttata
//