LOCUS       BBa_K2041007            1796 bp    DNA     linear       30-Dec-2025
DEFINITION  pbad-RBS-Bxb1-T
ACCESSION   BBa_K2041007
VERSION     BBa_K2041007.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..1659
                     /label=Mycobacterium phage Bxb1 integrase CDS
     CDS             157..1659
                     /label=BBa_K907000
     stem_loop       1679..1722
                     /label=stem_loop
     misc_feature    1668..1747
                     /label=BBa_B0010
     terminator      1668..1747
                     /label=BBa_B0010
     stem_loop       1763..1782
                     /label=T7 TE
     misc_feature    1789..1789
                     /label=stop
     polyA_site      1783..1796
                     /label=polya
     misc_feature    1756..1796
                     /label=BBa_B0012
     terminator      1756..1796
                     /label=BBa_B0012
     terminator      1668..1796
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga gagccctggt agtcatccgc
      181 ctgtcccgcg tcaccgatgc tacgacttca ccggagcgtc agctggagtc ttgccagcag
      241 ctctgcgccc agcgcggctg ggacgtcgtc ggggtagcgg aggatctgga cgtctccggg
      301 gcggtcgatc cgttcgaccg gaagcgcaga ccgaacctgg cccggtggct agcgttcgag
      361 gagcaaccgt tcgacgtgat cgtggcgtac cgggtagacc ggttgacccg atcgatccgg
      421 catctgcaac agctggtcca ctgggccgag gaccacaaga agctggtcgt ctccgcgacc
      481 gaagcgcact tcgatacgac gacgccgttt gcggcggtcg tcatcgcgct tatgggaacg
      541 gtggcgcaga tggaattaga agcgatcaaa gagcggaacc gttcggctgc gcatttcaat
      601 atccgcgccg ggaaataccg aggatccctg ccgccgtggg gatacctgcc tacgcgcgtg
      661 gacggggagt ggcggctggt gccggaccct gtgcagcgag agcgcatcct cgaggtgtat
      721 caccgcgtcg tcgacaacca cgagccgctg cacctggtgg cccacgacct gaaccggcgt
      781 ggtgtcctgt cgccgaagga ctacttcgcg cagctgcaag gccgcgagcc gcagggccgg
      841 gagtggtcgg ctaccgcgct gaagcgatcg atgatctccg aggcgatgct cgggtacgcg
      901 actctgaacg gtaagaccgt ccgagacgac gacggagccc cgctggtgcg ggctgagccg
      961 atcctgaccc gtgagcagct ggaggcgctg cgcgccgagc tcgtgaagac ctcccgggcg
     1021 aagcccgcgg tgtctacccc gtcgctgctg ctgcgggtgt tgttctgcgc ggtgtgcggg
     1081 gagcccgcgt acaagttcgc cgggggagga cgtaagcacc cgcgctaccg ctgccgctcg
     1141 atggggttcc cgaagcactg cgggaacggc acggtggcga tggccgagtg ggacgcgttc
     1201 tgcgaggagc aggtgctgga tctgctcggg gacgcggagc gtctggagaa agtctgggta
     1261 gccggctcgg actccgcggt cgaactcgcg gaggtgaacg cggagctggt ggacctgacg
     1321 tcgctgatcg gctccccggc ctaccgggcc ggctctccgc agcgagaagc actggatgcc
     1381 cgtattgcgg cgctggccgc gcggcaagag gagctggagg gcctagaggc tcgcccgtct
     1441 ggctgggagt ggcgcgagac cgggcagcgg ttcggggact ggtggcggga gcaggacacc
     1501 gcggcaaaga acacctggct tcggtcgatg aacgttcggc tgacgttcga cgtccgcggc
     1561 gggctgactc gcacgatcga cttcggggat ctgcaagagt acgagcagca tctcaggctc
     1621 ggcagcgtgg tcgaacggct acacaccggg atgtcgtagt actagagcca ggcatcaaat
     1681 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1741 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//