LOCUS       BBa_K2041008            1866 bp    DNA     linear       14-May-2024
DEFINITION  plac-RBS-Bxb1-T
ACCESSION   BBa_K2041008
VERSION     BBa_K2041008.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1729
                     /label=Mycobacterium phage Bxb1 integrase CDS
     CDS             227..1729
                     /label=BBa_K907000
     stem_loop       1749..1792
                     /label=stem_loop
     misc_feature    1738..1817
                     /label=BBa_B0010
     terminator      1738..1817
                     /label=BBa_B0010
     stem_loop       1833..1852
                     /label=T7 TE
     misc_feature    1859..1859
                     /label=stop
     polyA_site      1853..1866
                     /label=polya
     misc_feature    1826..1866
                     /label=BBa_B0012
     terminator      1826..1866
                     /label=BBa_B0012
     terminator      1738..1866
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gagccctggt
      241 agtcatccgc ctgtcccgcg tcaccgatgc tacgacttca ccggagcgtc agctggagtc
      301 ttgccagcag ctctgcgccc agcgcggctg ggacgtcgtc ggggtagcgg aggatctgga
      361 cgtctccggg gcggtcgatc cgttcgaccg gaagcgcaga ccgaacctgg cccggtggct
      421 agcgttcgag gagcaaccgt tcgacgtgat cgtggcgtac cgggtagacc ggttgacccg
      481 atcgatccgg catctgcaac agctggtcca ctgggccgag gaccacaaga agctggtcgt
      541 ctccgcgacc gaagcgcact tcgatacgac gacgccgttt gcggcggtcg tcatcgcgct
      601 tatgggaacg gtggcgcaga tggaattaga agcgatcaaa gagcggaacc gttcggctgc
      661 gcatttcaat atccgcgccg ggaaataccg aggatccctg ccgccgtggg gatacctgcc
      721 tacgcgcgtg gacggggagt ggcggctggt gccggaccct gtgcagcgag agcgcatcct
      781 cgaggtgtat caccgcgtcg tcgacaacca cgagccgctg cacctggtgg cccacgacct
      841 gaaccggcgt ggtgtcctgt cgccgaagga ctacttcgcg cagctgcaag gccgcgagcc
      901 gcagggccgg gagtggtcgg ctaccgcgct gaagcgatcg atgatctccg aggcgatgct
      961 cgggtacgcg actctgaacg gtaagaccgt ccgagacgac gacggagccc cgctggtgcg
     1021 ggctgagccg atcctgaccc gtgagcagct ggaggcgctg cgcgccgagc tcgtgaagac
     1081 ctcccgggcg aagcccgcgg tgtctacccc gtcgctgctg ctgcgggtgt tgttctgcgc
     1141 ggtgtgcggg gagcccgcgt acaagttcgc cgggggagga cgtaagcacc cgcgctaccg
     1201 ctgccgctcg atggggttcc cgaagcactg cgggaacggc acggtggcga tggccgagtg
     1261 ggacgcgttc tgcgaggagc aggtgctgga tctgctcggg gacgcggagc gtctggagaa
     1321 agtctgggta gccggctcgg actccgcggt cgaactcgcg gaggtgaacg cggagctggt
     1381 ggacctgacg tcgctgatcg gctccccggc ctaccgggcc ggctctccgc agcgagaagc
     1441 actggatgcc cgtattgcgg cgctggccgc gcggcaagag gagctggagg gcctagaggc
     1501 tcgcccgtct ggctgggagt ggcgcgagac cgggcagcgg ttcggggact ggtggcggga
     1561 gcaggacacc gcggcaaaga acacctggct tcggtcgatg aacgttcggc tgacgttcga
     1621 cgtccgcggc gggctgactc gcacgatcga cttcggggat ctgcaagagt acgagcagca
     1681 tctcaggctc ggcagcgtgg tcgaacggct acacaccggg atgtcgtagt actagagcca
     1741 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1801 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1861 tttata
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