LOCUS       BBa_K2041009            1720 bp    DNA     linear       14-May-2024
DEFINITION  ptet-RBS-Bxb1-T
ACCESSION   BBa_K2041009
VERSION     BBa_K2041009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1583
                     /label=Mycobacterium phage Bxb1 integrase CDS
     CDS             81..1583
                     /label=BBa_K907000
     stem_loop       1603..1646
                     /label=stem_loop
     misc_feature    1592..1671
                     /label=BBa_B0010
     terminator      1592..1671
                     /label=BBa_B0010
     stem_loop       1687..1706
                     /label=T7 TE
     misc_feature    1713..1713
                     /label=stop
     polyA_site      1707..1720
                     /label=polya
     misc_feature    1680..1720
                     /label=BBa_B0012
     terminator      1680..1720
                     /label=BBa_B0012
     terminator      1592..1720
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgagagccc tggtagtcat ccgcctgtcc cgcgtcaccg
      121 atgctacgac ttcaccggag cgtcagctgg agtcttgcca gcagctctgc gcccagcgcg
      181 gctgggacgt cgtcggggta gcggaggatc tggacgtctc cggggcggtc gatccgttcg
      241 accggaagcg cagaccgaac ctggcccggt ggctagcgtt cgaggagcaa ccgttcgacg
      301 tgatcgtggc gtaccgggta gaccggttga cccgatcgat ccggcatctg caacagctgg
      361 tccactgggc cgaggaccac aagaagctgg tcgtctccgc gaccgaagcg cacttcgata
      421 cgacgacgcc gtttgcggcg gtcgtcatcg cgcttatggg aacggtggcg cagatggaat
      481 tagaagcgat caaagagcgg aaccgttcgg ctgcgcattt caatatccgc gccgggaaat
      541 accgaggatc cctgccgccg tggggatacc tgcctacgcg cgtggacggg gagtggcggc
      601 tggtgccgga ccctgtgcag cgagagcgca tcctcgaggt gtatcaccgc gtcgtcgaca
      661 accacgagcc gctgcacctg gtggcccacg acctgaaccg gcgtggtgtc ctgtcgccga
      721 aggactactt cgcgcagctg caaggccgcg agccgcaggg ccgggagtgg tcggctaccg
      781 cgctgaagcg atcgatgatc tccgaggcga tgctcgggta cgcgactctg aacggtaaga
      841 ccgtccgaga cgacgacgga gccccgctgg tgcgggctga gccgatcctg acccgtgagc
      901 agctggaggc gctgcgcgcc gagctcgtga agacctcccg ggcgaagccc gcggtgtcta
      961 ccccgtcgct gctgctgcgg gtgttgttct gcgcggtgtg cggggagccc gcgtacaagt
     1021 tcgccggggg aggacgtaag cacccgcgct accgctgccg ctcgatgggg ttcccgaagc
     1081 actgcgggaa cggcacggtg gcgatggccg agtgggacgc gttctgcgag gagcaggtgc
     1141 tggatctgct cggggacgcg gagcgtctgg agaaagtctg ggtagccggc tcggactccg
     1201 cggtcgaact cgcggaggtg aacgcggagc tggtggacct gacgtcgctg atcggctccc
     1261 cggcctaccg ggccggctct ccgcagcgag aagcactgga tgcccgtatt gcggcgctgg
     1321 ccgcgcggca agaggagctg gagggcctag aggctcgccc gtctggctgg gagtggcgcg
     1381 agaccgggca gcggttcggg gactggtggc gggagcagga caccgcggca aagaacacct
     1441 ggcttcggtc gatgaacgtt cggctgacgt tcgacgtccg cggcgggctg actcgcacga
     1501 tcgacttcgg ggatctgcaa gagtacgagc agcatctcag gctcggcagc gtggtcgaac
     1561 ggctacacac cgggatgtcg tagtactaga gccaggcatc aaataaaacg aaaggctcag
     1621 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1681 cacactggct caccttcggg tgggcctttc tgcgtttata
//