LOCUS BBa_K2041010 4933 bp DNA linear 22-Dec-2024 DEFINITION plac-RBS-Bxb1-T-plux-RBS-luxR-T-plux-recognition site of Bxb1-RBS-luxI-RBS-GFP-T ACCESSION BBa_K2041010 VERSION BBa_K2041010.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1729 /label=Mycobacterium phage Bxb1 integrase CDS CDS 227..1729 /label=BBa_K907000 stem_loop 1749..1792 /label=stem_loop misc_feature 1738..1817 /label=BBa_B0010 terminator 1738..1817 /label=BBa_B0010 stem_loop 1833..1852 /label=T7 TE misc_feature 1859..1859 /label=stop polyA_site 1853..1866 /label=polya misc_feature 1826..1866 /label=BBa_B0012 terminator 1826..1866 /label=BBa_B0012 terminator 1738..1866 /label=BBa_B0015 stem_loop 2852..2895 /label=stem_loop misc_feature 2841..2920 /label=BBa_B0010 terminator 2841..2920 /label=BBa_B0010 stem_loop 2936..2955 /label=T7 TE misc_feature 2962..2962 /label=stop polyA_site 2956..2969 /label=polya misc_feature 2929..2969 /label=BBa_B0012 terminator 2929..2969 /label=BBa_B0012 terminator 2841..2969 /label=BBa_B0015 misc_feature 1875..1894 /label=LuxR/HSL promoter 1989..1996 /label=-10 promoter 1963..1968 /label=-35 misc_feature 2002..2002 /label=start misc_feature 2004..2023 /label=Putative LuxR/HSL misc_feature 1875..2025 /label=BBa_R0063 promoter 1875..2025 /label=BBa_R0063 misc_feature 2038..2041 /label=conserved RBS 2034..2045 /label=BBa_B0034 misc_feature 2052..2053 /label=prefix misc_feature 2225..2225 /label=T misc_feature 2543..2543 /label=A CDS 2052..2801 /label=luxR misc_feature 2052..2807 /label=BBa_C0062 misc_feature 2808..2832 /label=Help:Barcodes CDS 2052..2807 /label=BBa_C0062 stem_loop 2852..2895 /label=stem_loop misc_feature 2841..2920 /label=BBa_B0010 terminator 2841..2920 /label=BBa_B0010 stem_loop 2936..2955 /label=T7 TE misc_feature 2962..2962 /label=stop polyA_site 2956..2969 /label=polya misc_feature 2929..2969 /label=BBa_B0012 terminator 2929..2969 /label=BBa_B0012 terminator 2841..2969 /label=BBa_B0015 misc_feature 2978..2997 /label=LuxR/HSL promoter 2997..3002 /label=-35 promoter 3019..3024 /label=-10 misc_feature 3030..3030 /label=start misc_feature 2978..3032 /label=BBa_R0062 promoter 2978..3032 /label=BBa_R0062 misc_feature 2034..3032 /label=BBa_K317037 misc_feature 1875..3032 /label=BBa_K546003 misc_feature 3041..3067 /label=BBa_I11032 promoter 3076..3110 /label=BBa_J23104 misc_feature 3119..3378 /label=BBa_I11033 misc_feature 3391..3394 /label=conserved RBS 3387..3398 /label=BBa_B0034 misc_feature 3984..4015 /label=LVA CDS 3405..3983 /label=luxI misc_feature 3405..4022 /label=BBa_C0061 misc_feature 4023..4047 /label=Help:Barcodes CDS 3405..4022 /label=BBa_C0061 RBS 4056..4070 /label=RBS-1\Strong RBS 4063..4067 /label=RBS misc_feature 4056..4070 /label=BBa_B0030 RBS 4056..4070 /label=BBa_B0030 CDS 4077..4796 /label=GFP protein CDS 4077..4796 /label=BBa_E0040 stem_loop 4816..4859 /label=stem_loop misc_feature 4805..4884 /label=BBa_B0010 terminator 4805..4884 /label=BBa_B0010 stem_loop 4900..4919 /label=T7 TE misc_feature 4926..4926 /label=stop polyA_site 4920..4933 /label=polya misc_feature 4893..4933 /label=BBa_B0012 terminator 4893..4933 /label=BBa_B0012 misc_feature 4056..4933 /label=BBa_E0840 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gagccctggt 241 agtcatccgc ctgtcccgcg tcaccgatgc tacgacttca ccggagcgtc agctggagtc 301 ttgccagcag ctctgcgccc agcgcggctg ggacgtcgtc ggggtagcgg aggatctgga 361 cgtctccggg gcggtcgatc cgttcgaccg gaagcgcaga ccgaacctgg cccggtggct 421 agcgttcgag gagcaaccgt tcgacgtgat cgtggcgtac cgggtagacc ggttgacccg 481 atcgatccgg catctgcaac agctggtcca ctgggccgag gaccacaaga agctggtcgt 541 ctccgcgacc gaagcgcact tcgatacgac gacgccgttt gcggcggtcg tcatcgcgct 601 tatgggaacg gtggcgcaga tggaattaga agcgatcaaa gagcggaacc gttcggctgc 661 gcatttcaat atccgcgccg ggaaataccg aggatccctg ccgccgtggg gatacctgcc 721 tacgcgcgtg gacggggagt ggcggctggt gccggaccct gtgcagcgag agcgcatcct 781 cgaggtgtat caccgcgtcg tcgacaacca cgagccgctg cacctggtgg cccacgacct 841 gaaccggcgt ggtgtcctgt cgccgaagga ctacttcgcg cagctgcaag gccgcgagcc 901 gcagggccgg gagtggtcgg ctaccgcgct gaagcgatcg atgatctccg aggcgatgct 961 cgggtacgcg actctgaacg gtaagaccgt ccgagacgac gacggagccc cgctggtgcg 1021 ggctgagccg atcctgaccc gtgagcagct ggaggcgctg cgcgccgagc tcgtgaagac 1081 ctcccgggcg aagcccgcgg tgtctacccc gtcgctgctg ctgcgggtgt tgttctgcgc 1141 ggtgtgcggg gagcccgcgt acaagttcgc cgggggagga cgtaagcacc cgcgctaccg 1201 ctgccgctcg atggggttcc cgaagcactg cgggaacggc acggtggcga tggccgagtg 1261 ggacgcgttc tgcgaggagc aggtgctgga tctgctcggg gacgcggagc gtctggagaa 1321 agtctgggta gccggctcgg actccgcggt cgaactcgcg gaggtgaacg cggagctggt 1381 ggacctgacg tcgctgatcg gctccccggc ctaccgggcc ggctctccgc agcgagaagc 1441 actggatgcc cgtattgcgg cgctggccgc gcggcaagag gagctggagg gcctagaggc 1501 tcgcccgtct ggctgggagt ggcgcgagac cgggcagcgg ttcggggact ggtggcggga 1561 gcaggacacc gcggcaaaga acacctggct tcggtcgatg aacgttcggc tgacgttcga 1621 cgtccgcggc gggctgactc gcacgatcga cttcggggat ctgcaagagt acgagcagca 1681 tctcaggctc ggcagcgtgg tcgaacggct acacaccggg atgtcgtagt actagagcca 1741 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 1801 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 1861 tttatatact agagacctgt acgatcctac aggtgcttat gttaagtaat tgtattccca 1921 gcgatacaat agtgtgacaa aaatccaatt tattagaatc aaatgtcaat ccattaccgt 1981 tttaatgata tataacacgc aaaacttgcg acaaacaata ggtaatacta gagaaagagg 2041 agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta 2101 aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac 2161 attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt 2221 caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa 2281 aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat tggaatatat 2341 ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag 2401 gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta 2461 gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga 2521 acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata 2581 ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag 2641 gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc 2701 atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag 2761 caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct 2821 agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 2881 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 2941 accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta 3001 cgcaagaaaa tggtttgtta tagtcgaata aatactagag acgaccttcg cattacgaat 3061 gcgctgctac tagagttgac agctagctca gtcctaggta ttgtgctagc tactagagct 3121 aagtggtttg ggacaaaaat gggacataca aatctttgca tcggtttgca aggctttgca 3181 tgtctttcga agatgggacg tgtgagcgca ggtatgacgt ggtatgttgt tgacttaaaa 3241 ggtagttctt ataattcgta atgcgaaggt cgtaggttcg actcctatta tcggcaccag 3301 ttaaatcaaa tacttacgta ttattcgtgc cttccttatt tttactgtgg gacatatttg 3361 ggacagaagt accaaaaata ctagagaaag aggagaaata ctagatgact ataatgataa 3421 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 3481 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 3541 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 3601 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 3661 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 3721 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 3781 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 3841 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 3901 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 3961 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 4021 aactctgata gtgctagtgt agatctctac tagagattaa agaggagaaa tactagatgc 4081 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg 4141 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac 4201 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca 4261 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg 4321 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag 4381 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata 4441 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg 4501 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 4561 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt 4621 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt 4681 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg 4741 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact 4801 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 4861 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 4921 ttctgcgttt ata //