##gff-version 3
##sequence-region BBa_K2041010 1 4933
BBa_K2041010	.	CDS	3405	4022	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K2041010	.	engineered_region	3405	4022	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K2041010	.	tag	3984	4015	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K2041010	.	CDS	3405	3983	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K2041010	.	engineered_tag	4023	4047	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K2041010	.	terminator	2841	2969	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2041010	.	terminator	2841	2920	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2041010	.	stem_loop	2852	2895	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2041010	.	engineered_region	2841	2920	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2041010	.	terminator	2929	2969	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2041010	.	stem_loop	2936	2955	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2041010	.	stop_codon	2962	2962	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2041010	.	polyA_site	2956	2969	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2041010	.	engineered_region	2929	2969	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2041010	.	engineered_region	1875	3032	.	+	0	ID=BBa_K546003;Name=BBa_K546003
BBa_K2041010	.	engineered_region	2034	3032	.	+	0	ID=BBa_K317037;Name=BBa_K317037;Parent=BBa_K546003
BBa_K2041010	.	terminator	2841	2969	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K317037
BBa_K2041010	.	terminator	2841	2920	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2041010	.	stem_loop	2852	2895	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2041010	.	engineered_region	2841	2920	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2041010	.	terminator	2929	2969	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2041010	.	stem_loop	2936	2955	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2041010	.	stop_codon	2962	2962	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2041010	.	polyA_site	2956	2969	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2041010	.	engineered_region	2929	2969	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2041010	.	promoter	2978	3032	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K317037
BBa_K2041010	.	engineered_region	2978	3032	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K2041010	.	start_codon	3030	3030	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K2041010	.	promoter	3019	3024	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K2041010	.	non_covalent_binding_site	2978	2997	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K2041010	.	promoter	2997	3002	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K2041010	.	CDS	2052	2807	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K317037
BBa_K2041010	.	engineered_region	2052	2053	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K2041010	.	engineered_tag	2808	2832	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K2041010	.	point_mutation	2543	2543	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K2041010	.	engineered_region	2052	2807	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K2041010	.	point_mutation	2225	2225	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K2041010	.	CDS	2052	2801	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K2041010	.	ribosome_entry_site	2034	2045	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K317037
BBa_K2041010	.	conserved_region	2038	2041	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2041010	.	promoter	1875	2025	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_K546003
BBa_K2041010	.	promoter	1963	1968	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K2041010	.	non_covalent_binding_site	2004	2023	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K2041010	.	non_covalent_binding_site	1875	1894	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K2041010	.	engineered_region	1875	2025	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K2041010	.	start_codon	2002	2002	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K2041010	.	promoter	1989	1996	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K2041010	.	ribosome_entry_site	3387	3398	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2041010	.	conserved_region	3391	3394	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2041010	.	promoter	3076	3110	.	+	0	ID=BBa_J23104;Name=BBa_J23104
BBa_K2041010	.	CDS	227	1729	.	+	0	ID=BBa_K907000;Name=BBa_K907000
BBa_K2041010	.	CDS	227	1729	.	+	0	ID=annotation2187533;Name=Mycobacterium phage Bxb1 integrase CDS;Parent=BBa_K907000
BBa_K2041010	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2041010	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2041010	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K2041010	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K2041010	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K2041010	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K2041010	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K2041010	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K2041010	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K2041010	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K2041010	.	engineered_region	4056	4933	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K2041010	.	terminator	4893	4933	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K2041010	.	stem_loop	4900	4919	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2041010	.	stop_codon	4926	4926	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2041010	.	polyA_site	4920	4933	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2041010	.	engineered_region	4893	4933	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2041010	.	terminator	4805	4884	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K2041010	.	stem_loop	4816	4859	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2041010	.	engineered_region	4805	4884	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2041010	.	ribosome_entry_site	4056	4070	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K2041010	.	ribosome_entry_site	4056	4070	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2041010	.	ribosome_entry_site	4063	4067	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2041010	.	engineered_region	4056	4070	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2041010	.	CDS	4077	4796	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K2041010	.	CDS	4077	4796	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K2041010	.	sequence_feature	3119	3378	.	+	0	ID=BBa_I11033;Name=BBa_I11033
BBa_K2041010	.	terminator	1738	1866	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2041010	.	terminator	1738	1817	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2041010	.	stem_loop	1749	1792	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2041010	.	engineered_region	1738	1817	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2041010	.	terminator	1826	1866	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2041010	.	stem_loop	1833	1852	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2041010	.	stop_codon	1859	1859	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2041010	.	polyA_site	1853	1866	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2041010	.	engineered_region	1826	1866	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2041010	.	sequence_feature	3041	3067	.	+	0	ID=BBa_I11032;Name=BBa_I11032
>BBa_K2041010
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagagccct
ggtagtcatccgcctgtcccgcgtcaccgatgctacgacttcaccggagcgtcagctggagtcttgccagcagctctgc
gcccagcgcggctgggacgtcgtcggggtagcggaggatctggacgtctccggggcggtcgatccgttcgaccggaagc
gcagaccgaacctggcccggtggctagcgttcgaggagcaaccgttcgacgtgatcgtggcgtaccgggtagaccggtt
gacccgatcgatccggcatctgcaacagctggtccactgggccgaggaccacaagaagctggtcgtctccgcgaccgaa
gcgcacttcgatacgacgacgccgtttgcggcggtcgtcatcgcgcttatgggaacggtggcgcagatggaattagaag
cgatcaaagagcggaaccgttcggctgcgcatttcaatatccgcgccgggaaataccgaggatccctgccgccgtgggg
atacctgcctacgcgcgtggacggggagtggcggctggtgccggaccctgtgcagcgagagcgcatcctcgaggtgtat
caccgcgtcgtcgacaaccacgagccgctgcacctggtggcccacgacctgaaccggcgtggtgtcctgtcgccgaagg
actacttcgcgcagctgcaaggccgcgagccgcagggccgggagtggtcggctaccgcgctgaagcgatcgatgatctc
cgaggcgatgctcgggtacgcgactctgaacggtaagaccgtccgagacgacgacggagccccgctggtgcgggctgag
ccgatcctgacccgtgagcagctggaggcgctgcgcgccgagctcgtgaagacctcccgggcgaagcccgcggtgtcta
ccccgtcgctgctgctgcgggtgttgttctgcgcggtgtgcggggagcccgcgtacaagttcgccgggggaggacgtaa
gcacccgcgctaccgctgccgctcgatggggttcccgaagcactgcgggaacggcacggtggcgatggccgagtgggac
gcgttctgcgaggagcaggtgctggatctgctcggggacgcggagcgtctggagaaagtctgggtagccggctcggact
ccgcggtcgaactcgcggaggtgaacgcggagctggtggacctgacgtcgctgatcggctccccggcctaccgggccgg
ctctccgcagcgagaagcactggatgcccgtattgcggcgctggccgcgcggcaagaggagctggagggcctagaggct
cgcccgtctggctgggagtggcgcgagaccgggcagcggttcggggactggtggcgggagcaggacaccgcggcaaaga
acacctggcttcggtcgatgaacgttcggctgacgttcgacgtccgcggcgggctgactcgcacgatcgacttcgggga
tctgcaagagtacgagcagcatctcaggctcggcagcgtggtcgaacggctacacaccgggatgtcgtagtactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgc
ttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccatt
accgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatg
aaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaat
gcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatc
tgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcct
atagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctc
caaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggctt
cggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatacca
ttaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaa
gagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcg
tactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaatt
ttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacg
caagaaaatggtttgttatagtcgaataaatactagagacgaccttcgcattacgaatgcgctgctactagagttgaca
gctagctcagtcctaggtattgtgctagctactagagctaagtggtttgggacaaaaatgggacatacaaatctttgca
tcggtttgcaaggctttgcatgtctttcgaagatgggacgtgtgagcgcaggtatgacgtggtatgttgttgacttaaa
aggtagttcttataattcgtaatgcgaaggtcgtaggttcgactcctattatcggcaccagttaaatcaaatacttacg
tattattcgtgccttccttatttttactgtgggacatatttgggacagaagtaccaaaaatactagagaaagaggagaa
atactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtctt
cgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataact
caaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtga
ttatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagt
cgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatat
ataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtat
taaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcct
attaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgat
agtgctagtgtagatctctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagt
tgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgca
acatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactt
tcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccga
aggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggt
gatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaat
acaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagaca
caacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttta
ccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttg
agtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata