LOCUS BBa_K2041011 3270 bp DNA linear 16-May-2024 DEFINITION ptet-RBS-tetR-fimE-T-pbad-RBS-Bxb1-T ACCESSION BBa_K2041011 VERSION BBa_K2041011.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..700 /label=tetR misc_feature 701..734 /label=SsrA misc_feature 741..765 /label=Help:Barcodes CDS 81..765 /label=BBa_C0040 CDS 772..1329 /label=BBa_K137007 stem_loop 1349..1392 /label=stem_loop misc_feature 1338..1417 /label=BBa_B0010 terminator 1338..1417 /label=BBa_B0010 stem_loop 1433..1452 /label=T7 TE misc_feature 1459..1459 /label=stop polyA_site 1453..1466 /label=polya misc_feature 1426..1466 /label=BBa_B0012 terminator 1426..1466 /label=BBa_B0012 terminator 1338..1466 /label=BBa_B0015 promoter 1475..1605 /label=promoter misc_feature 1514..1531 /label=AraI1 misc_feature 1535..1552 /label=AraI2 promoter 1475..1604 /label=BBa_K206000 misc_feature 1617..1620 /label=conserved RBS 1613..1624 /label=BBa_B0034 CDS 1631..3133 /label=Mycobacterium phage Bxb1 integrase CDS CDS 1631..3133 /label=BBa_K907000 stem_loop 3153..3196 /label=stem_loop misc_feature 3142..3221 /label=BBa_B0010 terminator 3142..3221 /label=BBa_B0010 stem_loop 3237..3256 /label=T7 TE misc_feature 3263..3263 /label=stop polyA_site 3257..3270 /label=polya misc_feature 3230..3270 /label=BBa_B0012 terminator 3230..3270 /label=BBa_B0012 terminator 3142..3270 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat 121 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc 181 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg 241 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa 301 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc 361 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg 421 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac 481 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg 541 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta 601 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca 661 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact 721 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gatgatgcag 781 gcggtttgtt acggggcaac gggagccaga gattattgtc ttattctgtt ggcatatcgg 841 catgggatgc gtattagtga actgcttgat ctgcattatc aggaccttga ccttaatgaa 901 ggtagaataa atattcgccg actgaagaac ggattttcta ccgttcaccc gttacgtttt 961 gatgagcgtg aagccgtgga acgctggacc caggaacgtg ctaactggaa aggcgctgac 1021 cggactgacg ctatatttat ttctcgccgc gggagtcggc tttctcgcca gcaggcctat 1081 cgcattattc gcgatgccgg tattgaagct ggaaccgtaa cgcagactca tcctcatatg 1141 ttaaggcatg cttgcggtta tgaattggcg gagcgtggtg cagatactcg tttaattcag 1201 gattatctcg ggcatcgaaa tattcgccat actgtgcgtt ataccgccag taatgctgct 1261 cgttttgccg gattatggga aagaaataat ctcataaacg aaaaattaaa aagagaagag 1321 gtttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1381 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1441 ttcgggtggg cctttctgcg tttatatact agagacattg attatttgca cggcgtcaca 1501 ctttgctatg ccatagcaag atagtccata agattagcgg atcctacctg acgcttttta 1561 tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag agaaagagga 1621 gaaatactag atgagagccc tggtagtcat ccgcctgtcc cgcgtcaccg atgctacgac 1681 ttcaccggag cgtcagctgg agtcttgcca gcagctctgc gcccagcgcg gctgggacgt 1741 cgtcggggta gcggaggatc tggacgtctc cggggcggtc gatccgttcg accggaagcg 1801 cagaccgaac ctggcccggt ggctagcgtt cgaggagcaa ccgttcgacg tgatcgtggc 1861 gtaccgggta gaccggttga cccgatcgat ccggcatctg caacagctgg tccactgggc 1921 cgaggaccac aagaagctgg tcgtctccgc gaccgaagcg cacttcgata cgacgacgcc 1981 gtttgcggcg gtcgtcatcg cgcttatggg aacggtggcg cagatggaat tagaagcgat 2041 caaagagcgg aaccgttcgg ctgcgcattt caatatccgc gccgggaaat accgaggatc 2101 cctgccgccg tggggatacc tgcctacgcg cgtggacggg gagtggcggc tggtgccgga 2161 ccctgtgcag cgagagcgca tcctcgaggt gtatcaccgc gtcgtcgaca accacgagcc 2221 gctgcacctg gtggcccacg acctgaaccg gcgtggtgtc ctgtcgccga aggactactt 2281 cgcgcagctg caaggccgcg agccgcaggg ccgggagtgg tcggctaccg cgctgaagcg 2341 atcgatgatc tccgaggcga tgctcgggta cgcgactctg aacggtaaga ccgtccgaga 2401 cgacgacgga gccccgctgg tgcgggctga gccgatcctg acccgtgagc agctggaggc 2461 gctgcgcgcc gagctcgtga agacctcccg ggcgaagccc gcggtgtcta ccccgtcgct 2521 gctgctgcgg gtgttgttct gcgcggtgtg cggggagccc gcgtacaagt tcgccggggg 2581 aggacgtaag cacccgcgct accgctgccg ctcgatgggg ttcccgaagc actgcgggaa 2641 cggcacggtg gcgatggccg agtgggacgc gttctgcgag gagcaggtgc tggatctgct 2701 cggggacgcg gagcgtctgg agaaagtctg ggtagccggc tcggactccg cggtcgaact 2761 cgcggaggtg aacgcggagc tggtggacct gacgtcgctg atcggctccc cggcctaccg 2821 ggccggctct ccgcagcgag aagcactgga tgcccgtatt gcggcgctgg ccgcgcggca 2881 agaggagctg gagggcctag aggctcgccc gtctggctgg gagtggcgcg agaccgggca 2941 gcggttcggg gactggtggc gggagcagga caccgcggca aagaacacct ggcttcggtc 3001 gatgaacgtt cggctgacgt tcgacgtccg cggcgggctg actcgcacga tcgacttcgg 3061 ggatctgcaa gagtacgagc agcatctcag gctcggcagc gtggtcgaac ggctacacac 3121 cgggatgtcg tagtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3181 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3241 caccttcggg tgggcctttc tgcgtttata //