##gff-version 3
##sequence-region BBa_K2041011 1 3270
BBa_K2041011	.	CDS	1631	3133	.	+	0	ID=BBa_K907000;Name=BBa_K907000
BBa_K2041011	.	CDS	1631	3133	.	+	0	ID=annotation2187533;Name=Mycobacterium phage Bxb1 integrase CDS;Parent=BBa_K907000
BBa_K2041011	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2041011	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2041011	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2041011	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2041011	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2041011	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2041011	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2041011	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2041011	.	terminator	3142	3270	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2041011	.	terminator	3142	3221	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2041011	.	engineered_region	3142	3221	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2041011	.	stem_loop	3153	3196	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2041011	.	terminator	3230	3270	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2041011	.	stem_loop	3237	3256	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2041011	.	stop_codon	3263	3263	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2041011	.	engineered_region	3230	3270	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2041011	.	polyA_site	3257	3270	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2041011	.	ribosome_entry_site	1613	1624	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2041011	.	conserved_region	1617	1620	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2041011	.	promoter	1475	1604	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K2041011	.	promoter	1475	1605	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K2041011	.	operator	1514	1531	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K2041011	.	operator	1535	1552	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K2041011	.	CDS	772	1329	.	+	0	ID=BBa_K137007;Name=BBa_K137007
BBa_K2041011	.	CDS	81	765	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K2041011	.	tag	701	734	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K2041011	.	engineered_tag	741	765	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K2041011	.	CDS	84	700	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K2041011	.	terminator	1338	1466	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2041011	.	terminator	1338	1417	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2041011	.	engineered_region	1338	1417	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2041011	.	stem_loop	1349	1392	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2041011	.	terminator	1426	1466	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2041011	.	stem_loop	1433	1452	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2041011	.	stop_codon	1459	1459	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2041011	.	engineered_region	1426	1466	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2041011	.	polyA_site	1453	1466	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K2041011
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagatgatgcaggcggtttgtt
acggggcaacgggagccagagattattgtcttattctgttggcatatcggcatgggatgcgtattagtgaactgcttga
tctgcattatcaggaccttgaccttaatgaaggtagaataaatattcgccgactgaagaacggattttctaccgttcac
ccgttacgttttgatgagcgtgaagccgtggaacgctggacccaggaacgtgctaactggaaaggcgctgaccggactg
acgctatatttatttctcgccgcgggagtcggctttctcgccagcaggcctatcgcattattcgcgatgccggtattga
agctggaaccgtaacgcagactcatcctcatatgttaaggcatgcttgcggttatgaattggcggagcgtggtgcagat
actcgtttaattcaggattatctcgggcatcgaaatattcgccatactgtgcgttataccgccagtaatgctgctcgtt
ttgccggattatgggaaagaaataatctcataaacgaaaaattaaaaagagaagaggtttaataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacac
tttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagagccctggtagtcatccgcctgtc
ccgcgtcaccgatgctacgacttcaccggagcgtcagctggagtcttgccagcagctctgcgcccagcgcggctgggac
gtcgtcggggtagcggaggatctggacgtctccggggcggtcgatccgttcgaccggaagcgcagaccgaacctggccc
ggtggctagcgttcgaggagcaaccgttcgacgtgatcgtggcgtaccgggtagaccggttgacccgatcgatccggca
tctgcaacagctggtccactgggccgaggaccacaagaagctggtcgtctccgcgaccgaagcgcacttcgatacgacg
acgccgtttgcggcggtcgtcatcgcgcttatgggaacggtggcgcagatggaattagaagcgatcaaagagcggaacc
gttcggctgcgcatttcaatatccgcgccgggaaataccgaggatccctgccgccgtggggatacctgcctacgcgcgt
ggacggggagtggcggctggtgccggaccctgtgcagcgagagcgcatcctcgaggtgtatcaccgcgtcgtcgacaac
cacgagccgctgcacctggtggcccacgacctgaaccggcgtggtgtcctgtcgccgaaggactacttcgcgcagctgc
aaggccgcgagccgcagggccgggagtggtcggctaccgcgctgaagcgatcgatgatctccgaggcgatgctcgggta
cgcgactctgaacggtaagaccgtccgagacgacgacggagccccgctggtgcgggctgagccgatcctgacccgtgag
cagctggaggcgctgcgcgccgagctcgtgaagacctcccgggcgaagcccgcggtgtctaccccgtcgctgctgctgc
gggtgttgttctgcgcggtgtgcggggagcccgcgtacaagttcgccgggggaggacgtaagcacccgcgctaccgctg
ccgctcgatggggttcccgaagcactgcgggaacggcacggtggcgatggccgagtgggacgcgttctgcgaggagcag
gtgctggatctgctcggggacgcggagcgtctggagaaagtctgggtagccggctcggactccgcggtcgaactcgcgg
aggtgaacgcggagctggtggacctgacgtcgctgatcggctccccggcctaccgggccggctctccgcagcgagaagc
actggatgcccgtattgcggcgctggccgcgcggcaagaggagctggagggcctagaggctcgcccgtctggctgggag
tggcgcgagaccgggcagcggttcggggactggtggcgggagcaggacaccgcggcaaagaacacctggcttcggtcga
tgaacgttcggctgacgttcgacgtccgcggcgggctgactcgcacgatcgacttcggggatctgcaagagtacgagca
gcatctcaggctcggcagcgtggtcgaacggctacacaccgggatgtcgtagtactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata