LOCUS       BBa_K2041013            1229 bp    DNA     linear       22-Nov-2024
DEFINITION  ptet-key3d-RBS-ptet-lock3-RBS-GFP-T
ACCESSION   BBa_K2041013
VERSION     BBa_K2041013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..159
                     /label=BBa_J23009
     misc_feature    172..175
                     /label=conserved
     RBS             168..179
                     /label=BBa_B0034
     misc_feature    188..206
                     /label=TetR 1
     misc_feature    188..241
                     /label=BBa_R0040
     promoter        207..212
                     /label=-35
     misc_feature    213..231
                     /label=TetR 2
     promoter        230..235
                     /label=-10
     promoter        188..241
                     /label=BBa_R0040
     misc_feature    250..343
                     /label=BBa_J23008
     RBS             352..366
                     /label=RBS-1\Strong
     RBS             359..363
                     /label=RBS
     misc_feature    352..366
                     /label=BBa_B0030
     RBS             352..366
                     /label=BBa_B0030
     CDS             373..1092
                     /label=GFP protein
     CDS             373..1092
                     /label=BBa_E0040
     stem_loop       1112..1155
                     /label=stem_loop
     misc_feature    1101..1180
                     /label=BBa_B0010
     terminator      1101..1180
                     /label=BBa_B0010
     stem_loop       1196..1215
                     /label=T7 TE
     misc_feature    1222..1222
                     /label=stop
     polyA_site      1216..1229
                     /label=polya
     misc_feature    1189..1229
                     /label=BBa_B0012
     terminator      1189..1229
                     /label=BBa_B0012
     misc_feature    352..1229
                     /label=BBa_E0840
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agcgggcggg cgggcgggcg ggatccataa aaaaaaaacc caaaagcaag aggtgattct
      121 agttaaaaaa aaaaagcttc ccgcccgccc gcccgcccgt actagagaaa gaggagaaat
      181 actagagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca
      241 ctactagaga cccaaaagca agaggtgatt ctagttggtg gttaatgaaa attaacttac
      301 ttactagaaa tatctctaaa aagccagatt attaatccgg ctttactaga gattaaagag
      361 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      421 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      481 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      541 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
      601 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      661 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
      721 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      781 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
      841 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
      901 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      961 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1021 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     1081 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1141 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1201 accttcgggt gggcctttct gcgtttata
//