##gff-version 3
##sequence-region BBa_K2041013 1 1229
BBa_K2041013	.	ribosome_entry_site	168	179	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2041013	.	conserved_region	172	175	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2041013	.	promoter	188	241	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2041013	.	promoter	207	212	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2041013	.	non_covalent_binding_site	213	231	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2041013	.	engineered_region	188	241	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2041013	.	non_covalent_binding_site	188	206	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2041013	.	promoter	230	235	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2041013	.	mature_transcript_region	250	343	.	+	0	ID=BBa_J23008;Name=BBa_J23008
BBa_K2041013	.	mature_transcript_region	63	159	.	+	0	ID=BBa_J23009;Name=BBa_J23009
BBa_K2041013	.	engineered_region	352	1229	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K2041013	.	ribosome_entry_site	352	366	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K2041013	.	engineered_region	352	366	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2041013	.	ribosome_entry_site	352	366	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2041013	.	ribosome_entry_site	359	363	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2041013	.	CDS	373	1092	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K2041013	.	CDS	373	1092	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K2041013	.	terminator	1101	1180	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K2041013	.	stem_loop	1112	1155	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2041013	.	engineered_region	1101	1180	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2041013	.	terminator	1189	1229	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K2041013	.	engineered_region	1189	1229	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2041013	.	stop_codon	1222	1222	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2041013	.	polyA_site	1216	1229	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2041013	.	stem_loop	1196	1215	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2041013	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2041013	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2041013	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2041013	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2041013	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2041013	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_K2041013
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagcgggcgggcgggcgggc
gggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgccc
gtactagagaaagaggagaaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactga
gcactactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctc
taaaaagccagattattaatccggctttactagagattaaagaggagaaatactagatgcgtaaaggagaagaactttt
cactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaa
ggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttg
tcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgc
catgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaag
tttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacaca
aattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaa
aattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccct
gtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatgg
tccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata