LOCUS       BBa_K2041014             982 bp    DNA     linear       18-May-2024
DEFINITION  key3d-RBS-ptet-lock3-fimE-T
ACCESSION   BBa_K2041014
VERSION     BBa_K2041014.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_J23009
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     misc_feature    126..144
                     /label=TetR 1
     misc_feature    126..179
                     /label=BBa_R0040
     promoter        145..150
                     /label=-35
     misc_feature    151..169
                     /label=TetR 2
     promoter        168..173
                     /label=-10
     promoter        126..179
                     /label=BBa_R0040
     misc_feature    188..281
                     /label=BBa_J23008
     CDS             288..845
                     /label=BBa_K137007
     stem_loop       865..908
                     /label=stem_loop
     misc_feature    854..933
                     /label=BBa_B0010
     terminator      854..933
                     /label=BBa_B0010
     stem_loop       949..968
                     /label=T7 TE
     misc_feature    975..975
                     /label=stop
     polyA_site      969..982
                     /label=polya
     misc_feature    942..982
                     /label=BBa_B0012
     terminator      942..982
                     /label=BBa_B0012
     terminator      854..982
                     /label=BBa_B0015
ORIGIN
        1 cgggcgggcg ggcgggcggg atccataaaa aaaaaaccca aaagcaagag gtgattctag
       61 ttaaaaaaaa aaagcttccc gcccgcccgc ccgcccgtac tagagaaaga ggagaaatac
      121 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcact
      181 actagagacc caaaagcaag aggtgattct agttggtggt taatgaaaat taacttactt
      241 actagaaata tctctaaaaa gccagattat taatccggct ttactagatg atgcaggcgg
      301 tttgttacgg ggcaacggga gccagagatt attgtcttat tctgttggca tatcggcatg
      361 ggatgcgtat tagtgaactg cttgatctgc attatcagga ccttgacctt aatgaaggta
      421 gaataaatat tcgccgactg aagaacggat tttctaccgt tcacccgtta cgttttgatg
      481 agcgtgaagc cgtggaacgc tggacccagg aacgtgctaa ctggaaaggc gctgaccgga
      541 ctgacgctat atttatttct cgccgcggga gtcggctttc tcgccagcag gcctatcgca
      601 ttattcgcga tgccggtatt gaagctggaa ccgtaacgca gactcatcct catatgttaa
      661 ggcatgcttg cggttatgaa ttggcggagc gtggtgcaga tactcgttta attcaggatt
      721 atctcgggca tcgaaatatt cgccatactg tgcgttatac cgccagtaat gctgctcgtt
      781 ttgccggatt atgggaaaga aataatctca taaacgaaaa attaaaaaga gaagaggttt
      841 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      961 ggtgggcctt tctgcgttta ta
//