LOCUS       BBa_K2041015            1044 bp    DNA     linear       22-May-2024
DEFINITION  ptet-key3d-RBS-ptet-lock3-fimE-T
ACCESSION   BBa_K2041015
VERSION     BBa_K2041015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..159
                     /label=BBa_J23009
     misc_feature    172..175
                     /label=conserved
     RBS             168..179
                     /label=BBa_B0034
     misc_feature    188..206
                     /label=TetR 1
     misc_feature    188..241
                     /label=BBa_R0040
     promoter        207..212
                     /label=-35
     misc_feature    213..231
                     /label=TetR 2
     promoter        230..235
                     /label=-10
     promoter        188..241
                     /label=BBa_R0040
     misc_feature    250..343
                     /label=BBa_J23008
     CDS             350..907
                     /label=BBa_K137007
     stem_loop       927..970
                     /label=stem_loop
     misc_feature    916..995
                     /label=BBa_B0010
     terminator      916..995
                     /label=BBa_B0010
     stem_loop       1011..1030
                     /label=T7 TE
     misc_feature    1037..1037
                     /label=stop
     polyA_site      1031..1044
                     /label=polya
     misc_feature    1004..1044
                     /label=BBa_B0012
     terminator      1004..1044
                     /label=BBa_B0012
     terminator      916..1044
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agcgggcggg cgggcgggcg ggatccataa aaaaaaaacc caaaagcaag aggtgattct
      121 agttaaaaaa aaaaagcttc ccgcccgccc gcccgcccgt actagagaaa gaggagaaat
      181 actagagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca
      241 ctactagaga cccaaaagca agaggtgatt ctagttggtg gttaatgaaa attaacttac
      301 ttactagaaa tatctctaaa aagccagatt attaatccgg ctttactaga tgatgcaggc
      361 ggtttgttac ggggcaacgg gagccagaga ttattgtctt attctgttgg catatcggca
      421 tgggatgcgt attagtgaac tgcttgatct gcattatcag gaccttgacc ttaatgaagg
      481 tagaataaat attcgccgac tgaagaacgg attttctacc gttcacccgt tacgttttga
      541 tgagcgtgaa gccgtggaac gctggaccca ggaacgtgct aactggaaag gcgctgaccg
      601 gactgacgct atatttattt ctcgccgcgg gagtcggctt tctcgccagc aggcctatcg
      661 cattattcgc gatgccggta ttgaagctgg aaccgtaacg cagactcatc ctcatatgtt
      721 aaggcatgct tgcggttatg aattggcgga gcgtggtgca gatactcgtt taattcagga
      781 ttatctcggg catcgaaata ttcgccatac tgtgcgttat accgccagta atgctgctcg
      841 ttttgccgga ttatgggaaa gaaataatct cataaacgaa aaattaaaaa gagaagaggt
      901 ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      961 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1021 cgggtgggcc tttctgcgtt tata
//