LOCUS BBa_K2041016 4889 bp DNA linear 13-May-2024 DEFINITION FimE and Bxb1 device regulated by ptet and pbad ACCESSION BBa_K2041016 VERSION BBa_K2041016.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..700 /label=tetR misc_feature 701..734 /label=SsrA misc_feature 741..765 /label=Help:Barcodes CDS 81..765 /label=BBa_C0040 CDS 772..1329 /label=BBa_K137007 stem_loop 1349..1392 /label=stem_loop misc_feature 1338..1417 /label=BBa_B0010 terminator 1338..1417 /label=BBa_B0010 stem_loop 1433..1452 /label=T7 TE misc_feature 1459..1459 /label=stop polyA_site 1453..1466 /label=polya misc_feature 1426..1466 /label=BBa_B0012 terminator 1426..1466 /label=BBa_B0012 terminator 1338..1466 /label=BBa_B0015 promoter 1475..1605 /label=promoter misc_feature 1514..1531 /label=AraI1 misc_feature 1535..1552 /label=AraI2 promoter 1475..1604 /label=BBa_K206000 misc_feature 1617..1620 /label=conserved RBS 1613..1624 /label=BBa_B0034 CDS 1631..3133 /label=Mycobacterium phage Bxb1 integrase CDS CDS 1631..3133 /label=BBa_K907000 stem_loop 3153..3196 /label=stem_loop misc_feature 3142..3221 /label=BBa_B0010 terminator 3142..3221 /label=BBa_B0010 stem_loop 3237..3256 /label=T7 TE misc_feature 3263..3263 /label=stop polyA_site 3257..3270 /label=polya misc_feature 3230..3270 /label=BBa_B0012 terminator 3230..3270 /label=BBa_B0012 terminator 3142..3270 /label=BBa_B0015 promoter 3279..3313 /label=BBa_J23104 misc_feature 3322..3338 /label=IRR out misc_feature 3340..3356 /label=IRR in misc_feature 3322..3356 /label=BBa_K137008 promoter 3365..3418 /label=tetR promoter promoter 3365..3614 /label=BBa_K137047 misc_feature 3623..3639 /label=IRL in misc_feature 3641..3657 /label=IRL out misc_feature 3623..3657 /label=BBa_K137010 misc_feature 3666..3692 /label=BBa_I11032 promoter 3701..3735 /label=BBa_J23104 misc_feature 3744..4003 /label=BBa_I11033 RBS 4012..4026 /label=RBS-1\Strong RBS 4019..4023 /label=RBS misc_feature 4012..4026 /label=BBa_B0030 RBS 4012..4026 /label=BBa_B0030 CDS 4033..4752 /label=GFP protein CDS 4033..4752 /label=BBa_E0040 stem_loop 4772..4815 /label=stem_loop misc_feature 4761..4840 /label=BBa_B0010 terminator 4761..4840 /label=BBa_B0010 stem_loop 4856..4875 /label=T7 TE misc_feature 4882..4882 /label=stop polyA_site 4876..4889 /label=polya misc_feature 4849..4889 /label=BBa_B0012 terminator 4849..4889 /label=BBa_B0012 misc_feature 4012..4889 /label=BBa_E0840 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat 121 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc 181 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg 241 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa 301 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc 361 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg 421 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac 481 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg 541 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta 601 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca 661 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact 721 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gatgatgcag 781 gcggtttgtt acggggcaac gggagccaga gattattgtc ttattctgtt ggcatatcgg 841 catgggatgc gtattagtga actgcttgat ctgcattatc aggaccttga ccttaatgaa 901 ggtagaataa atattcgccg actgaagaac ggattttcta ccgttcaccc gttacgtttt 961 gatgagcgtg aagccgtgga acgctggacc caggaacgtg ctaactggaa aggcgctgac 1021 cggactgacg ctatatttat ttctcgccgc gggagtcggc tttctcgcca gcaggcctat 1081 cgcattattc gcgatgccgg tattgaagct ggaaccgtaa cgcagactca tcctcatatg 1141 ttaaggcatg cttgcggtta tgaattggcg gagcgtggtg cagatactcg tttaattcag 1201 gattatctcg ggcatcgaaa tattcgccat actgtgcgtt ataccgccag taatgctgct 1261 cgttttgccg gattatggga aagaaataat ctcataaacg aaaaattaaa aagagaagag 1321 gtttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1381 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1441 ttcgggtggg cctttctgcg tttatatact agagacattg attatttgca cggcgtcaca 1501 ctttgctatg ccatagcaag atagtccata agattagcgg atcctacctg acgcttttta 1561 tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag agaaagagga 1621 gaaatactag atgagagccc tggtagtcat ccgcctgtcc cgcgtcaccg atgctacgac 1681 ttcaccggag cgtcagctgg agtcttgcca gcagctctgc gcccagcgcg gctgggacgt 1741 cgtcggggta gcggaggatc tggacgtctc cggggcggtc gatccgttcg accggaagcg 1801 cagaccgaac ctggcccggt ggctagcgtt cgaggagcaa ccgttcgacg tgatcgtggc 1861 gtaccgggta gaccggttga cccgatcgat ccggcatctg caacagctgg tccactgggc 1921 cgaggaccac aagaagctgg tcgtctccgc gaccgaagcg cacttcgata cgacgacgcc 1981 gtttgcggcg gtcgtcatcg cgcttatggg aacggtggcg cagatggaat tagaagcgat 2041 caaagagcgg aaccgttcgg ctgcgcattt caatatccgc gccgggaaat accgaggatc 2101 cctgccgccg tggggatacc tgcctacgcg cgtggacggg gagtggcggc tggtgccgga 2161 ccctgtgcag cgagagcgca tcctcgaggt gtatcaccgc gtcgtcgaca accacgagcc 2221 gctgcacctg gtggcccacg acctgaaccg gcgtggtgtc ctgtcgccga aggactactt 2281 cgcgcagctg caaggccgcg agccgcaggg ccgggagtgg tcggctaccg cgctgaagcg 2341 atcgatgatc tccgaggcga tgctcgggta cgcgactctg aacggtaaga ccgtccgaga 2401 cgacgacgga gccccgctgg tgcgggctga gccgatcctg acccgtgagc agctggaggc 2461 gctgcgcgcc gagctcgtga agacctcccg ggcgaagccc gcggtgtcta ccccgtcgct 2521 gctgctgcgg gtgttgttct gcgcggtgtg cggggagccc gcgtacaagt tcgccggggg 2581 aggacgtaag cacccgcgct accgctgccg ctcgatgggg ttcccgaagc actgcgggaa 2641 cggcacggtg gcgatggccg agtgggacgc gttctgcgag gagcaggtgc tggatctgct 2701 cggggacgcg gagcgtctgg agaaagtctg ggtagccggc tcggactccg cggtcgaact 2761 cgcggaggtg aacgcggagc tggtggacct gacgtcgctg atcggctccc cggcctaccg 2821 ggccggctct ccgcagcgag aagcactgga tgcccgtatt gcggcgctgg ccgcgcggca 2881 agaggagctg gagggcctag aggctcgccc gtctggctgg gagtggcgcg agaccgggca 2941 gcggttcggg gactggtggc gggagcagga caccgcggca aagaacacct ggcttcggtc 3001 gatgaacgtt cggctgacgt tcgacgtccg cggcgggctg actcgcacga tcgacttcgg 3061 ggatctgcaa gagtacgagc agcatctcag gctcggcagc gtggtcgaac ggctacacac 3121 cgggatgtcg tagtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3181 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3241 caccttcggg tgggcctttc tgcgtttata tactagagtt gacagctagc tcagtcctag 3301 gtattgtgct agctactaga gaagatgaaa catttggggc caaactgtcc atattatact 3361 agaggtgctc agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggact 3421 ctagtatata aacgcagaaa ggcccacccg aaggtgagcc agtgtgactc tagtagagag 3481 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt 3541 ttatttgatg cctggctcta gtagtgatct acactagcac tatcagtgtt attaagctac 3601 taaagcgtag tttttactag agtctatgag tcaaaatggc cccaattgtc ttgtatttac 3661 tagagacgac cttcgcatta cgaatgcgct gctactagag ttgacagcta gctcagtcct 3721 aggtattgtg ctagctacta gagctaagtg gtttgggaca aaaatgggac atacaaatct 3781 ttgcatcggt ttgcaaggct ttgcatgtct ttcgaagatg ggacgtgtga gcgcaggtat 3841 gacgtggtat gttgttgact taaaaggtag ttcttataat tcgtaatgcg aaggtcgtag 3901 gttcgactcc tattatcggc accagttaaa tcaaatactt acgtattatt cgtgccttcc 3961 ttatttttac tgtgggacat atttgggaca gaagtaccaa aaatactaga gattaaagag 4021 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt 4081 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat 4141 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca 4201 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat 4261 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga 4321 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt 4381 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt 4441 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa 4501 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt 4561 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca 4621 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac 4681 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta 4741 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 4801 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 4861 accttcgggt gggcctttct gcgtttata //