##gff-version 3
##sequence-region BBa_K2041016 1 4889
BBa_K2041016	.	ribosome_entry_site	1613	1624	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2041016	.	conserved_region	1617	1620	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2041016	.	terminator	1338	1466	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2041016	.	terminator	1338	1417	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2041016	.	stem_loop	1349	1392	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2041016	.	engineered_region	1338	1417	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2041016	.	terminator	1426	1466	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2041016	.	polyA_site	1453	1466	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2041016	.	stem_loop	1433	1452	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2041016	.	engineered_region	1426	1466	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2041016	.	stop_codon	1459	1459	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2041016	.	sequence_feature	3666	3692	.	+	0	ID=BBa_I11032;Name=BBa_I11032
BBa_K2041016	.	CDS	772	1329	.	+	0	ID=BBa_K137007;Name=BBa_K137007
BBa_K2041016	.	sequence_feature	3623	3657	.	+	0	ID=BBa_K137010;Name=BBa_K137010
BBa_K2041016	.	sequence_feature	3623	3639	.	+	0	ID=annotation1963843;Name=IRL in;Parent=BBa_K137010
BBa_K2041016	.	sequence_feature	3641	3657	.	+	0	ID=annotation1963844;Name=IRL out;Parent=BBa_K137010
BBa_K2041016	.	terminator	3142	3270	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2041016	.	terminator	3142	3221	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2041016	.	stem_loop	3153	3196	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2041016	.	engineered_region	3142	3221	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2041016	.	terminator	3230	3270	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2041016	.	polyA_site	3257	3270	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2041016	.	stem_loop	3237	3256	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2041016	.	engineered_region	3230	3270	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2041016	.	stop_codon	3263	3263	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2041016	.	CDS	81	765	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K2041016	.	CDS	84	700	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K2041016	.	tag	701	734	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K2041016	.	engineered_tag	741	765	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K2041016	.	sequence_feature	3744	4003	.	+	0	ID=BBa_I11033;Name=BBa_I11033
BBa_K2041016	.	CDS	1631	3133	.	+	0	ID=BBa_K907000;Name=BBa_K907000
BBa_K2041016	.	CDS	1631	3133	.	+	0	ID=annotation2187533;Name=Mycobacterium phage Bxb1 integrase CDS;Parent=BBa_K907000
BBa_K2041016	.	promoter	1475	1604	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K2041016	.	operator	1514	1531	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K2041016	.	promoter	1475	1605	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K2041016	.	operator	1535	1552	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K2041016	.	engineered_region	4012	4889	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K2041016	.	terminator	4849	4889	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K2041016	.	polyA_site	4876	4889	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2041016	.	stem_loop	4856	4875	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2041016	.	engineered_region	4849	4889	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2041016	.	stop_codon	4882	4882	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2041016	.	CDS	4033	4752	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K2041016	.	CDS	4033	4752	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K2041016	.	terminator	4761	4840	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K2041016	.	stem_loop	4772	4815	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2041016	.	engineered_region	4761	4840	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2041016	.	ribosome_entry_site	4012	4026	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K2041016	.	ribosome_entry_site	4012	4026	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2041016	.	ribosome_entry_site	4019	4023	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2041016	.	engineered_region	4012	4026	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2041016	.	promoter	3701	3735	.	+	0	ID=BBa_J23104;Name=BBa_J23104
BBa_K2041016	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2041016	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2041016	.	sequence_feature	3322	3356	.	+	0	ID=BBa_K137008;Name=BBa_K137008
BBa_K2041016	.	sequence_feature	3322	3338	.	+	0	ID=annotation1963841;Name=IRR out;Parent=BBa_K137008
BBa_K2041016	.	sequence_feature	3340	3356	.	+	0	ID=annotation1963842;Name=IRR in;Parent=BBa_K137008
BBa_K2041016	.	promoter	3365	3614	.	+	0	ID=BBa_K137047;Name=BBa_K137047
BBa_K2041016	.	promoter	3365	3418	.	+	0	ID=annotation1968020;Name=tetR promoter;Parent=BBa_K137047
BBa_K2041016	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2041016	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2041016	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2041016	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2041016	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2041016	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2041016	.	promoter	3279	3313	.	+	0	ID=BBa_J23104;Name=BBa_J23104
>BBa_K2041016
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagatgatgcaggcggtttgtt
acggggcaacgggagccagagattattgtcttattctgttggcatatcggcatgggatgcgtattagtgaactgcttga
tctgcattatcaggaccttgaccttaatgaaggtagaataaatattcgccgactgaagaacggattttctaccgttcac
ccgttacgttttgatgagcgtgaagccgtggaacgctggacccaggaacgtgctaactggaaaggcgctgaccggactg
acgctatatttatttctcgccgcgggagtcggctttctcgccagcaggcctatcgcattattcgcgatgccggtattga
agctggaaccgtaacgcagactcatcctcatatgttaaggcatgcttgcggttatgaattggcggagcgtggtgcagat
actcgtttaattcaggattatctcgggcatcgaaatattcgccatactgtgcgttataccgccagtaatgctgctcgtt
ttgccggattatgggaaagaaataatctcataaacgaaaaattaaaaagagaagaggtttaataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacac
tttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagagccctggtagtcatccgcctgtc
ccgcgtcaccgatgctacgacttcaccggagcgtcagctggagtcttgccagcagctctgcgcccagcgcggctgggac
gtcgtcggggtagcggaggatctggacgtctccggggcggtcgatccgttcgaccggaagcgcagaccgaacctggccc
ggtggctagcgttcgaggagcaaccgttcgacgtgatcgtggcgtaccgggtagaccggttgacccgatcgatccggca
tctgcaacagctggtccactgggccgaggaccacaagaagctggtcgtctccgcgaccgaagcgcacttcgatacgacg
acgccgtttgcggcggtcgtcatcgcgcttatgggaacggtggcgcagatggaattagaagcgatcaaagagcggaacc
gttcggctgcgcatttcaatatccgcgccgggaaataccgaggatccctgccgccgtggggatacctgcctacgcgcgt
ggacggggagtggcggctggtgccggaccctgtgcagcgagagcgcatcctcgaggtgtatcaccgcgtcgtcgacaac
cacgagccgctgcacctggtggcccacgacctgaaccggcgtggtgtcctgtcgccgaaggactacttcgcgcagctgc
aaggccgcgagccgcagggccgggagtggtcggctaccgcgctgaagcgatcgatgatctccgaggcgatgctcgggta
cgcgactctgaacggtaagaccgtccgagacgacgacggagccccgctggtgcgggctgagccgatcctgacccgtgag
cagctggaggcgctgcgcgccgagctcgtgaagacctcccgggcgaagcccgcggtgtctaccccgtcgctgctgctgc
gggtgttgttctgcgcggtgtgcggggagcccgcgtacaagttcgccgggggaggacgtaagcacccgcgctaccgctg
ccgctcgatggggttcccgaagcactgcgggaacggcacggtggcgatggccgagtgggacgcgttctgcgaggagcag
gtgctggatctgctcggggacgcggagcgtctggagaaagtctgggtagccggctcggactccgcggtcgaactcgcgg
aggtgaacgcggagctggtggacctgacgtcgctgatcggctccccggcctaccgggccggctctccgcagcgagaagc
actggatgcccgtattgcggcgctggccgcgcggcaagaggagctggagggcctagaggctcgcccgtctggctgggag
tggcgcgagaccgggcagcggttcggggactggtggcgggagcaggacaccgcggcaaagaacacctggcttcggtcga
tgaacgttcggctgacgttcgacgtccgcggcgggctgactcgcacgatcgacttcggggatctgcaagagtacgagca
gcatctcaggctcggcagcgtggtcgaacggctacacaccgggatgtcgtagtactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtattgtgctagctacta
gagaagatgaaacatttggggccaaactgtccatattatactagaggtgctcagtatctctatcactgatagggatgtc
aatctctatcactgatagggactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtag
agagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg
ctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagtttttactagagtctatgagtcaa
aatggccccaattgtcttgtatttactagagacgaccttcgcattacgaatgcgctgctactagagttgacagctagct
cagtcctaggtattgtgctagctactagagctaagtggtttgggacaaaaatgggacatacaaatctttgcatcggttt
gcaaggctttgcatgtctttcgaagatgggacgtgtgagcgcaggtatgacgtggtatgttgttgacttaaaaggtagt
tcttataattcgtaatgcgaaggtcgtaggttcgactcctattatcggcaccagttaaatcaaatacttacgtattatt
cgtgccttccttatttttactgtgggacatatttgggacagaagtaccaaaaatactagagattaaagaggagaaatac
tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata