LOCUS       BBa_K2041021            1044 bp    DNA     linear       22-Dec-2024
DEFINITION  promoter-RBS-binding site of miR-155-GFP
ACCESSION   BBa_K2041021
VERSION     BBa_K2041021.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..86
                     /label=the binding site 1 of miR-155
     misc_feature    88..110
                     /label=the binding site 2 of miR-155
     misc_feature    136..158
                     /label=the binding site 4 of miR-155
     misc_feature    112..134
                     /label=the binding site 3 of miR-155
     misc_feature    64..158
                     /label=BBa_K2041018
     RBS             167..181
                     /label=RBS-1\Strong
     RBS             174..178
                     /label=RBS
     misc_feature    167..181
                     /label=BBa_B0030
     RBS             167..181
                     /label=BBa_B0030
     CDS             188..907
                     /label=GFP protein
     CDS             188..907
                     /label=BBa_E0040
     stem_loop       927..970
                     /label=stem_loop
     misc_feature    916..995
                     /label=BBa_B0010
     terminator      916..995
                     /label=BBa_B0010
     stem_loop       1011..1030
                     /label=T7 TE
     misc_feature    1037..1037
                     /label=stop
     polyA_site      1031..1044
                     /label=polya
     misc_feature    1004..1044
                     /label=BBa_B0012
     terminator      1004..1044
                     /label=BBa_B0012
     terminator      916..1044
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gagaccccta tcacgattag cattaagacc cctatcacga ttagcattaa gacccctatc
      121 acgattagca ttaagacccc tatcacgatt agcattaata ctagagatta aagaggagaa
      181 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      241 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      301 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      361 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
      421 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      481 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      541 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      601 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
      661 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
      721 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      781 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
      841 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
      901 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      961 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1021 cgggtgggcc tttctgcgtt tata
//