BBa_K2042001 1 BBa_K2042001 Pseudomonas resinovorans PHA synthase 1 2016-09-01T11:00:00Z 2016-10-10T09:13:24Z This sequence was synthetized by IDT This is the coding sequence for a Polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer. This sequence is codon optimized for pseudomonas putida it includes four mutations which were found to increase yields of PLA (Yang et al.) site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA); site-directed mutagenesis, the type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, i.e. E130, S325, S477, and Q481 We note that unlike BioBrick BBa K1211002 Yale 2013, one of the mutation is not on the Serine 477 on Serine 475. which is able to do the reaction: lactylCoA + PLA(n) = PLA(n+1). false false _2509_ 31392 31392 9 false We used this sequence as second enzyme in order to produce PLA. In fact it will take lactyl coA as substract and polymerise it into PLA homopolymere false Toky RATOVOMANANA annotation2512238 1 PhaC range2512238 1 1 1680 annotation2512239 1 Start range2512239 1 1 3 annotation2512256 1 Stop range2512256 1 1678 1680 BBa_K2042001_sequence 1 atgagcaacaagaacaacgaagacctccagcgtcaagcatccgacaacacgctgaatctcaaccccgtgattggcatccgcgggaaagatctgctcagctcggctcgcatggtgctgctccaggcaattaaacaaccattccactccgcaaagcatgtcgcgcatttcgggctcgagttgaagaacgtgctgctgggtcaaagcgggctccagcctgaagcagacgatcgccgttttaacgaccctgcctggtcccagaaccctctgtacaagcgctatctccaaacctatctcgcatggcgcaaagagctccacagctggatcgacgagagcaacctgtccagccaggacgcttcgcggggccatttcgtcattaacctgatgacggatgctatggcaccgaccaactccatggctaatccagcagctgtgaagcgcttttttgaaacgggcgggaagtccctgttggatggtctgtcgcatctggcaaaggacatggtcaacaatggcggtatgccttcccaggtcaatatggacgcgttcgaggtgggccaaaacctcgctacgacggagggtgctgtggtctttcggaatgatgtcttggaattgatccagtacaaacctattacggaatccgtgtatgagcggcctctcttggtcgtccctccccaaatcaataaattctatgtctttgacctgtccccggagaaatccctcgcgcgcttctgcctgcgtagcaacctccagacgtttatcgtgagctggcgtaaccctaccaaggcgcagcgggaatggggtctcagcacgtacatcgaggccctgaaagaagcgatcgacgtcattctcaagatcacgggtgctaaagacttgaatattttgggtgcttgttcgggtggcattaccacggtggctctcttgggccactaccaggcgatcggtgagacgaaggtgaatgctttcacgcaaatggtcaccgtgctcgatttcaacctggattcccaggtcgcactcttcgcggacgagcaaacgctcgaggctgcgaaacggcgttcgtatcaagccggcgtgctcgaagggaaagatatggctaaggtgtttgcctggatgcggccaaatgacttgatttggaactactgggtgaataattatctcttgggtaatgaacctcccgccttcgatattttgtattggaacaatgacacgacccgcctccctgctgcgttccacggcgagttggtggagatgttcaaaacgaatgcgttgacccgtccaaatgcgctcgaagtgtgtggcacgccaattgatttgaaacaggtgacgagcgattttttttgcttggcgggtacgaccgaccatattaccccttgggaagcatgttatcgctcggccttgctgctggggggcaagtgcgaatttgtcctgagcaatggggggcacatcaagtcgattctcaatccaccgggcaacccaaaagcccgcttttcgacgggtagcgaaatgcctaaggacccaaaagcatggttggagaatgcgaccaagcatgcagattcctggtggttgcactggcaacaatggattggtgagcggtcgggtaaaaccaagaaagcttcgttcaccctgggtaataaagccttccctgctggtgaagcaagccccggtacctatgtgcacgagcgttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z