BBa_K2042003 1 BBa_K2042003 RBS 2016-09-01T11:00:00Z 2016-09-11T05:02:27Z A A false false _2509_ 31392 31443 9 false A false Charlotte NAYRAC annotation2512505 1 RBS range2512505 1 1 7 BBa_K2042010 1 BBa_K2042010 RBS + Pct 2016-09-01T11:00:00Z 2016-10-12T03:07:19Z a a false false _2509_ 31392 32278 9 false a false Jonathan Tellechea component2482789 1 BBa_K2042003 component2482791 1 BBa_K2042000 component2482790 1 BBa_K2042016 annotation2482790 1 BBa_K2042016 range2482790 1 8 13 annotation2482789 1 BBa_K2042003 range2482789 1 1 7 annotation2482791 1 BBa_K2042000 range2482791 1 14 1588 BBa_K2042016 1 BBa_K2042016 Non-coding scar site in between RBS and gene 2016-09-10T11:00:00Z 2016-10-08T02:48:20Z Synthetic synthesis Nucleotide between RBS and Pct false false _2509_ 31392 31392 9 false None false Toky RATOVOMANANA annotation2488301 1 scar range2488301 1 1 6 BBa_K2042000 1 Pct Propionate CoA transferase for P. putida 2016-08-18T11:00:00Z 2016-10-15T05:56:21Z The wild type sequence is from Clostridium propionicum genome. This is the DNA coding sequence for the enzyme Propionate CoA. The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA. Transferase. This mutated sequence has one amino acid substitution: A243T (compared to the wild type enzyme) and is codon optimized for Pseudomonas putida in order to produce (D)-Lactyl-CoA. It extend the BBa_K1211001 biobrick from Yale 2013 and have the same purpose. false false _2509_ 31392 31392 9 false We use this enzyme as the first enzyme in the pathway to create PLA or Polylactic acid. Lactate can also act as acceptors for the enzyme. In our project, the enzyme will catalyze the reaction: acetyl-CoA + lactate <--> acetate + Lactyl-CoA. false Toky RATOVOMANANA annotation2512229 1 Stop range2512229 1 1573 1575 annotation2512227 1 Pct range2512227 1 1 1575 annotation2512235 1 mutation range2512235 1 727 728 annotation2512228 1 Start range2512228 1 1 3 annotation2512231 1 s_mutation range2512231 1 1446 1447 BBa_K2042016_sequence 1 ctgagg BBa_K2042000_sequence 1 atgcgtaaggtccctatcattaccgctgacgaagccgcgaaactgatcaaagatggggacacggtcaccaccagcggctttgtcggcaatgccattccggaggcgctggatcgcgctgtcgaaaaacgctttttggaaacgggcgagcccaaaaacatcacgtatgtgtactgtggttcccagggcaaccgcgacggtcggggcgcggagcattttgctcatgaaggcttgttgaagcgctatattgcggggcattgggctacggtcccggctctcgggaagatggcgatggagaacaaaatggaggcatacaatgtgtcgcagggggcactctgtcatctgttccgggatatcgcctcgcataagcccggcgtgtttacgaaagtgggcatcggtaccttcatcgaccctcggaacgggggcggtaaagtgaacgacatcacgaaagaagatatcgtcgaactggtcgaaatcaaggggcaggaatatttgttctaccccgcgtttcccatccatgtggcgctgatccgtggtacgtatgcggacgaatccggtaacattaccttcgagaaggaggtcgccccattggaaggcacgtccgtctgccaagcagtgaaaaactcgggggggatcgtcgtggtgcaagtggaacgggtcgtcaaagcgggcaccctggatccgcggcatgtcaaggtgccaggcatctacgtggactacgtcgtggtcaccgacccggaggatcaccaacagtcgctcgactgtgaatatgatccagcgctctccggcgaacaccggcgcccggaggtggtgggcgaacccctgcctctgtccgctaagaaagtcattggtcgtcgcggcgccattgagttggagaaagacgtcgccgtcaacctgggtgtcggtgcacccgagtacgtcgcgagcgtcgctgatgaggagggtattgtggatttcatgaccctgaccgctgaaagcggtgcaattggcggcgtcccagcgggtggcgtgcgcttcggtgcgtcctataatgcggacgccctgatcgatcaaggctatcagttcgattactatgatggcggtggtctcgatctctgttatctcgggttggctgaatgcgatgaaaaaggcaatattaacgtcagccgcttcggtccgcgcatcgccgggtgcggtggtttcattaatatcacccaaaacacccccaaggtgtttttttgtgggacgtttacggcgggcggtctgaaggtgaaaatcgaagatggtaaagtgatcatcgtccaggaggggaagcagaaaaagttcttgaaagccgtggagcagatcacgttcaacggggacgtggctctcgcaaataaacagcaagtcacgtacattacggagcgttgtgtcttcttgttgaaggaggatggtctgcacctcagcgaaatcgcgcctggtattgatctccagacccaaatcttggacgtgatggacttcgcaccgatcatcgaccgggatgcgaacgggcagatcaagctgatggacgctgcgctgtttgcagaagggctgatgggtctcaaggaaatgaaatcctga BBa_K2042003_sequence 1 gaaggag BBa_K2042010_sequence 1 gaaggagctgaggatgcgtaaggtccctatcattaccgctgacgaagccgcgaaactgatcaaagatggggacacggtcaccaccagcggctttgtcggcaatgccattccggaggcgctggatcgcgctgtcgaaaaacgctttttggaaacgggcgagcccaaaaacatcacgtatgtgtactgtggttcccagggcaaccgcgacggtcggggcgcggagcattttgctcatgaaggcttgttgaagcgctatattgcggggcattgggctacggtcccggctctcgggaagatggcgatggagaacaaaatggaggcatacaatgtgtcgcagggggcactctgtcatctgttccgggatatcgcctcgcataagcccggcgtgtttacgaaagtgggcatcggtaccttcatcgaccctcggaacgggggcggtaaagtgaacgacatcacgaaagaagatatcgtcgaactggtcgaaatcaaggggcaggaatatttgttctaccccgcgtttcccatccatgtggcgctgatccgtggtacgtatgcggacgaatccggtaacattaccttcgagaaggaggtcgccccattggaaggcacgtccgtctgccaagcagtgaaaaactcgggggggatcgtcgtggtgcaagtggaacgggtcgtcaaagcgggcaccctggatccgcggcatgtcaaggtgccaggcatctacgtggactacgtcgtggtcaccgacccggaggatcaccaacagtcgctcgactgtgaatatgatccagcgctctccggcgaacaccggcgcccggaggtggtgggcgaacccctgcctctgtccgctaagaaagtcattggtcgtcgcggcgccattgagttggagaaagacgtcgccgtcaacctgggtgtcggtgcacccgagtacgtcgcgagcgtcgctgatgaggagggtattgtggatttcatgaccctgaccgctgaaagcggtgcaattggcggcgtcccagcgggtggcgtgcgcttcggtgcgtcctataatgcggacgccctgatcgatcaaggctatcagttcgattactatgatggcggtggtctcgatctctgttatctcgggttggctgaatgcgatgaaaaaggcaatattaacgtcagccgcttcggtccgcgcatcgccgggtgcggtggtttcattaatatcacccaaaacacccccaaggtgtttttttgtgggacgtttacggcgggcggtctgaaggtgaaaatcgaagatggtaaagtgatcatcgtccaggaggggaagcagaaaaagttcttgaaagccgtggagcagatcacgttcaacggggacgtggctctcgcaaataaacagcaagtcacgtacattacggagcgttgtgtcttcttgttgaaggaggatggtctgcacctcagcgaaatcgcgcctggtattgatctccagacccaaatcttggacgtgatggacttcgcaccgatcatcgaccgggatgcgaacgggcagatcaagctgatggacgctgcgctgtttgcagaagggctgatgggtctcaaggaaatgaaatcctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z