BBa_K2042011 1 BBa_K2042011 RBS +PhaC_resinovorans 2016-09-01T11:00:00Z 2016-10-10T09:14:16Z a a false false _2509_ 31392 32278 9 false a false Jonathan Tellechea component2482793 1 BBa_K2042016 component2482792 1 BBa_K2042003 component2482794 1 BBa_K2042001 annotation2482792 1 BBa_K2042003 range2482792 1 1 7 annotation2482794 1 BBa_K2042001 range2482794 1 14 1693 annotation2482793 1 BBa_K2042016 range2482793 1 8 13 BBa_K2042016 1 BBa_K2042016 Non-coding scar site in between RBS and gene 2016-09-10T11:00:00Z 2016-10-08T02:48:20Z Synthetic synthesis Nucleotide between RBS and Pct false false _2509_ 31392 31392 9 false None false Toky RATOVOMANANA annotation2488301 1 scar range2488301 1 1 6 BBa_K2042003 1 BBa_K2042003 RBS 2016-09-01T11:00:00Z 2016-09-11T05:02:27Z A A false false _2509_ 31392 31443 9 false A false Charlotte NAYRAC annotation2512505 1 RBS range2512505 1 1 7 BBa_K2042001 1 BBa_K2042001 Pseudomonas resinovorans PHA synthase 1 2016-09-01T11:00:00Z 2016-10-10T09:13:24Z This sequence was synthetized by IDT This is the coding sequence for a Polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer. This sequence is codon optimized for pseudomonas putida it includes four mutations which were found to increase yields of PLA (Yang et al.) site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA); site-directed mutagenesis, the type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, i.e. E130, S325, S477, and Q481 We note that unlike BioBrick BBa K1211002 Yale 2013, one of the mutation is not on the Serine 477 on Serine 475. which is able to do the reaction: lactylCoA + PLA(n) = PLA(n+1). false false _2509_ 31392 31392 9 false We used this sequence as second enzyme in order to produce PLA. In fact it will take lactyl coA as substract and polymerise it into PLA homopolymere false Toky RATOVOMANANA annotation2512238 1 PhaC range2512238 1 1 1680 annotation2512256 1 Stop range2512256 1 1678 1680 annotation2512239 1 Start range2512239 1 1 3 BBa_K2042016_sequence 1 ctgagg BBa_K2042001_sequence 1 atgagcaacaagaacaacgaagacctccagcgtcaagcatccgacaacacgctgaatctcaaccccgtgattggcatccgcgggaaagatctgctcagctcggctcgcatggtgctgctccaggcaattaaacaaccattccactccgcaaagcatgtcgcgcatttcgggctcgagttgaagaacgtgctgctgggtcaaagcgggctccagcctgaagcagacgatcgccgttttaacgaccctgcctggtcccagaaccctctgtacaagcgctatctccaaacctatctcgcatggcgcaaagagctccacagctggatcgacgagagcaacctgtccagccaggacgcttcgcggggccatttcgtcattaacctgatgacggatgctatggcaccgaccaactccatggctaatccagcagctgtgaagcgcttttttgaaacgggcgggaagtccctgttggatggtctgtcgcatctggcaaaggacatggtcaacaatggcggtatgccttcccaggtcaatatggacgcgttcgaggtgggccaaaacctcgctacgacggagggtgctgtggtctttcggaatgatgtcttggaattgatccagtacaaacctattacggaatccgtgtatgagcggcctctcttggtcgtccctccccaaatcaataaattctatgtctttgacctgtccccggagaaatccctcgcgcgcttctgcctgcgtagcaacctccagacgtttatcgtgagctggcgtaaccctaccaaggcgcagcgggaatggggtctcagcacgtacatcgaggccctgaaagaagcgatcgacgtcattctcaagatcacgggtgctaaagacttgaatattttgggtgcttgttcgggtggcattaccacggtggctctcttgggccactaccaggcgatcggtgagacgaaggtgaatgctttcacgcaaatggtcaccgtgctcgatttcaacctggattcccaggtcgcactcttcgcggacgagcaaacgctcgaggctgcgaaacggcgttcgtatcaagccggcgtgctcgaagggaaagatatggctaaggtgtttgcctggatgcggccaaatgacttgatttggaactactgggtgaataattatctcttgggtaatgaacctcccgccttcgatattttgtattggaacaatgacacgacccgcctccctgctgcgttccacggcgagttggtggagatgttcaaaacgaatgcgttgacccgtccaaatgcgctcgaagtgtgtggcacgccaattgatttgaaacaggtgacgagcgattttttttgcttggcgggtacgaccgaccatattaccccttgggaagcatgttatcgctcggccttgctgctggggggcaagtgcgaatttgtcctgagcaatggggggcacatcaagtcgattctcaatccaccgggcaacccaaaagcccgcttttcgacgggtagcgaaatgcctaaggacccaaaagcatggttggagaatgcgaccaagcatgcagattcctggtggttgcactggcaacaatggattggtgagcggtcgggtaaaaccaagaaagcttcgttcaccctgggtaataaagccttccctgctggtgaagcaagccccggtacctatgtgcacgagcgttga BBa_K2042003_sequence 1 gaaggag BBa_K2042011_sequence 1 gaaggagctgaggatgagcaacaagaacaacgaagacctccagcgtcaagcatccgacaacacgctgaatctcaaccccgtgattggcatccgcgggaaagatctgctcagctcggctcgcatggtgctgctccaggcaattaaacaaccattccactccgcaaagcatgtcgcgcatttcgggctcgagttgaagaacgtgctgctgggtcaaagcgggctccagcctgaagcagacgatcgccgttttaacgaccctgcctggtcccagaaccctctgtacaagcgctatctccaaacctatctcgcatggcgcaaagagctccacagctggatcgacgagagcaacctgtccagccaggacgcttcgcggggccatttcgtcattaacctgatgacggatgctatggcaccgaccaactccatggctaatccagcagctgtgaagcgcttttttgaaacgggcgggaagtccctgttggatggtctgtcgcatctggcaaaggacatggtcaacaatggcggtatgccttcccaggtcaatatggacgcgttcgaggtgggccaaaacctcgctacgacggagggtgctgtggtctttcggaatgatgtcttggaattgatccagtacaaacctattacggaatccgtgtatgagcggcctctcttggtcgtccctccccaaatcaataaattctatgtctttgacctgtccccggagaaatccctcgcgcgcttctgcctgcgtagcaacctccagacgtttatcgtgagctggcgtaaccctaccaaggcgcagcgggaatggggtctcagcacgtacatcgaggccctgaaagaagcgatcgacgtcattctcaagatcacgggtgctaaagacttgaatattttgggtgcttgttcgggtggcattaccacggtggctctcttgggccactaccaggcgatcggtgagacgaaggtgaatgctttcacgcaaatggtcaccgtgctcgatttcaacctggattcccaggtcgcactcttcgcggacgagcaaacgctcgaggctgcgaaacggcgttcgtatcaagccggcgtgctcgaagggaaagatatggctaaggtgtttgcctggatgcggccaaatgacttgatttggaactactgggtgaataattatctcttgggtaatgaacctcccgccttcgatattttgtattggaacaatgacacgacccgcctccctgctgcgttccacggcgagttggtggagatgttcaaaacgaatgcgttgacccgtccaaatgcgctcgaagtgtgtggcacgccaattgatttgaaacaggtgacgagcgattttttttgcttggcgggtacgaccgaccatattaccccttgggaagcatgttatcgctcggccttgctgctggggggcaagtgcgaatttgtcctgagcaatggggggcacatcaagtcgattctcaatccaccgggcaacccaaaagcccgcttttcgacgggtagcgaaatgcctaaggacccaaaagcatggttggagaatgcgaccaagcatgcagattcctggtggttgcactggcaacaatggattggtgagcggtcgggtaaaaccaagaaagcttcgttcaccctgggtaataaagccttccctgctggtgaagcaagccccggtacctatgtgcacgagcgttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z