LOCUS       BBa_K2042014            3286 bp    DNA     linear       16-Jun-2024
DEFINITION  RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)
ACCESSION   BBa_K2042014
VERSION     BBa_K2042014.1  
FEATURES             Location/Qualifiers
     RBS             1..7
                     /label=RBS
     RBS             1..7
                     /label=BBa_K2042003
     misc_feature    8..13
                     /label=scar
     misc_feature    8..13
                     /label=BBa_K2042016
     CDS             14..1693
                     /label=PhaC
     misc_feature    14..16
                     /label=Start
     misc_feature    1691..1693
                     /label=Stop
     misc_feature    403..403
                     /label=E130D
     misc_feature    987..987
                     /label=Pst1 removed
     misc_feature    1454..1454
                     /label=Q481K
     misc_feature    1442..1442
                     /label=S477G
     misc_feature    1429..1429
                     /label=Pst1 removed
     misc_feature    987..987
                     /label=S325T
     CDS             14..1693
                     /label=BBa_K2042002
     misc_feature    1..1693
                     /label=BBa_K2042012
     misc_feature    1694..1698
                     /label=Scar
     misc_feature    1694..1698
                     /label=BBa_K2042017
     RBS             1699..1705
                     /label=RBS
     RBS             1699..1705
                     /label=BBa_K2042003
     misc_feature    1706..1711
                     /label=scar
     misc_feature    1706..1711
                     /label=BBa_K2042016
     CDS             1712..3286
                     /label=Pct
     misc_feature    1712..1714
                     /label=Start
     misc_feature    3284..3286
                     /label=Stop
     misc_feature    3157..3158
                     /label=s_mutation
     misc_feature    2438..2439
                     /label=mutation
     CDS             1712..3286
                     /label=BBa_K2042000
     misc_feature    1699..3286
                     /label=BBa_K2042010
ORIGIN
        1 gaaggagctg aggatgagta acaagagtaa cgatgagttg aagtatcaag cctctgaaaa
       61 caccttgggg cttaatcctg tcgttgggct gcgtggaaag gatctactgg cttctgctcg
      121 aatggtgctt aggcaggcca tcaagcaacc ggtgcacagc gtcaaacatg tcgcgcactt
      181 tggtcttgaa ctcaagaacg tactgctggg taaatccggg ctgcaaccga ccagcgatga
      241 ccgtcgcttc gccgatccgg cctggagcca gaacccgctc tataaacgtt atttgcaaac
      301 ctacctggcg tggcgcaagg aactccacga ctggatcgat gaaagtaacc tcgcccccaa
      361 ggatgtggcg cgtgggcact tcgtgatcaa cctcatgacc gatgccatgg cgccgaccaa
      421 caccgcggcc aacccggcgg cagtcaaacg ctttttcgaa accggtggca aaagcctgct
      481 cgacggcctc tcgcacctgg ccaaggatct ggtacacaac ggcggcatgc cgagccaggt
      541 caacatgggt gcattcgagg tcggcaagag cctgggcgtg accgaaggcg cggtggtgtt
      601 tcgcaacgat gtgctggaac tgatccagta caagccgacc accgagcagg tatacgaacg
      661 cccgctgctg gtggtgccgc cgcagatcaa caagttctac gttttcgacc tgagcccgga
      721 caagagcctg gcgcggttct gcctgcgcaa caacgtgcaa acgttcatcg tcagctggcg
      781 aaatcccacc aaggaacagc gagagtgggg cctgtcgacc tacatcgaag ccctcaagga
      841 agcggttgac gtcgttaccg cgatcaccgg cagcaaagac gtgaacatgc tcggggcctg
      901 ctccggcggc atcacttgca ctgcgctgct gggccattac gcggcgattg gcgaaaacaa
      961 ggtcaacgcc ctgaccttgc tggtgaccgt gcttgatacc accctcgaca gcgacgtcgc
     1021 cctgttcgtc aatgaacaga cccttgaagc cgccaagcgc cactcgtacc aggccggcgt
     1081 actggaaggc cgcgacatgg cgaaggtctt cgcctggatg cgccccaacg atctgatctg
     1141 gaactactgg gtcaacaatt acctgctagg caacgaaccg ccggtgttcg acatcctgtt
     1201 ctggaacaac gacaccacac ggttgcccgc ggcgttccac ggcgacctgg tcgaactgtt
     1261 caaaaataac ccactgattc gcccgaatgc actggaagtg tgcggcaccc ccatcgacct
     1321 caagcaggtg acggccgaca tcttttccct ggccggcacc aacgaccaca tcaccccgtg
     1381 gaagtcctgc tacaagtcgg cgcaactgtt tggcggcaac gttgaatttg tgctgtcgag
     1441 cggcgggcat atcaagagca tcctgaaccc gccgggcaat ccgaaatcgc gctacatgac
     1501 cagcaccgaa gtggcggaaa atgccgatga atggcaagcg aatgccacca agcatacaga
     1561 ttcctggtgg ctgcactggc aggcctggca ggcccaacgc tcgggcgagc tgaaaaagtc
     1621 cccgacaaaa ctgggcagca aggcgtatcc ggcaggtgaa gcggcgccag gcacgtacgt
     1681 gcacgaacgg taatactaga aggagctgag gatgcgtaag gtccctatca ttaccgctga
     1741 cgaagccgcg aaactgatca aagatgggga cacggtcacc accagcggct ttgtcggcaa
     1801 tgccattccg gaggcgctgg atcgcgctgt cgaaaaacgc tttttggaaa cgggcgagcc
     1861 caaaaacatc acgtatgtgt actgtggttc ccagggcaac cgcgacggtc ggggcgcgga
     1921 gcattttgct catgaaggct tgttgaagcg ctatattgcg gggcattggg ctacggtccc
     1981 ggctctcggg aagatggcga tggagaacaa aatggaggca tacaatgtgt cgcagggggc
     2041 actctgtcat ctgttccggg atatcgcctc gcataagccc ggcgtgttta cgaaagtggg
     2101 catcggtacc ttcatcgacc ctcggaacgg gggcggtaaa gtgaacgaca tcacgaaaga
     2161 agatatcgtc gaactggtcg aaatcaaggg gcaggaatat ttgttctacc ccgcgtttcc
     2221 catccatgtg gcgctgatcc gtggtacgta tgcggacgaa tccggtaaca ttaccttcga
     2281 gaaggaggtc gccccattgg aaggcacgtc cgtctgccaa gcagtgaaaa actcgggggg
     2341 gatcgtcgtg gtgcaagtgg aacgggtcgt caaagcgggc accctggatc cgcggcatgt
     2401 caaggtgcca ggcatctacg tggactacgt cgtggtcacc gacccggagg atcaccaaca
     2461 gtcgctcgac tgtgaatatg atccagcgct ctccggcgaa caccggcgcc cggaggtggt
     2521 gggcgaaccc ctgcctctgt ccgctaagaa agtcattggt cgtcgcggcg ccattgagtt
     2581 ggagaaagac gtcgccgtca acctgggtgt cggtgcaccc gagtacgtcg cgagcgtcgc
     2641 tgatgaggag ggtattgtgg atttcatgac cctgaccgct gaaagcggtg caattggcgg
     2701 cgtcccagcg ggtggcgtgc gcttcggtgc gtcctataat gcggacgccc tgatcgatca
     2761 aggctatcag ttcgattact atgatggcgg tggtctcgat ctctgttatc tcgggttggc
     2821 tgaatgcgat gaaaaaggca atattaacgt cagccgcttc ggtccgcgca tcgccgggtg
     2881 cggtggtttc attaatatca cccaaaacac ccccaaggtg tttttttgtg ggacgtttac
     2941 ggcgggcggt ctgaaggtga aaatcgaaga tggtaaagtg atcatcgtcc aggaggggaa
     3001 gcagaaaaag ttcttgaaag ccgtggagca gatcacgttc aacggggacg tggctctcgc
     3061 aaataaacag caagtcacgt acattacgga gcgttgtgtc ttcttgttga aggaggatgg
     3121 tctgcacctc agcgaaatcg cgcctggtat tgatctccag acccaaatct tggacgtgat
     3181 ggacttcgca ccgatcatcg accgggatgc gaacgggcag atcaagctga tggacgctgc
     3241 gctgtttgca gaagggctga tgggtctcaa ggaaatgaaa tcctga
//