BBa_K2042002 1 BBa_K2042002 Pseudomonas sp. MBEL 6-19 PHA synthase 1 2016-09-01T11:00:00Z 2016-10-15T02:38:07Z a a false false _2509_ 31392 31380 9 false a false Maria Bargu??s i Ribera annotation2512501 1 S477G range2512501 1 1429 1429 annotation2512500 1 Q481K range2512500 1 1441 1441 annotation2512498 1 E130D range2512498 1 390 390 annotation2512476 1 Start range2512476 1 1 3 annotation2512499 1 Pst1 removed range2512499 1 974 974 annotation2512502 1 Pst1 removed range2512502 1 1416 1416 annotation2512503 1 S325T range2512503 1 974 974 annotation2512477 1 Stop range2512477 1 1678 1680 annotation2512474 1 PhaC range2512474 1 1 1680 BBa_K2042014 1 BBa_K2042014 RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP) 2016-10-03T11:00:00Z 2016-10-14T03:28:59Z Enter before 2016 deadline RBS + Pct + RBS + PhaC_v4 false false _2509_ 31381 31392 9 false Enter before 2016 deadline false Toky RATOVOMANANA component2485991 1 BBa_K2042012 component2485996 1 BBa_K2042010 component2485992 1 BBa_K2042017 annotation2485992 1 BBa_K2042017 range2485992 1 1694 1698 annotation2485991 1 BBa_K2042012 range2485991 1 1 1693 annotation2485996 1 BBa_K2042010 range2485996 1 1699 3286 BBa_K2042003 1 BBa_K2042003 RBS 2016-09-01T11:00:00Z 2016-09-11T05:02:27Z A A false false _2509_ 31392 31443 9 false A false Charlotte NAYRAC annotation2512505 1 RBS range2512505 1 1 7 BBa_K2042000 1 Pct Propionate CoA transferase for P. putida 2016-08-18T11:00:00Z 2016-10-15T05:56:21Z The wild type sequence is from Clostridium propionicum genome. This is the DNA coding sequence for the enzyme Propionate CoA. The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA. Transferase. This mutated sequence has one amino acid substitution: A243T (compared to the wild type enzyme) and is codon optimized for Pseudomonas putida in order to produce (D)-Lactyl-CoA. It extend the BBa_K1211001 biobrick from Yale 2013 and have the same purpose. false false _2509_ 31392 31392 9 false We use this enzyme as the first enzyme in the pathway to create PLA or Polylactic acid. Lactate can also act as acceptors for the enzyme. In our project, the enzyme will catalyze the reaction: acetyl-CoA + lactate <--> acetate + Lactyl-CoA. false Toky RATOVOMANANA annotation2512231 1 s_mutation range2512231 1 1446 1447 annotation2512228 1 Start range2512228 1 1 3 annotation2512227 1 Pct range2512227 1 1 1575 annotation2512229 1 Stop range2512229 1 1573 1575 annotation2512235 1 mutation range2512235 1 727 728 BBa_K2042012 1 BBa_K2042012 RBS + PhaC_sp. MBEL 6-19 2016-09-02T11:00:00Z 2016-10-10T09:14:57Z a RBS + PhaC_v4 false false _2509_ 31392 31381 9 false a false Delphine Dessaux component2482800 1 BBa_K2042002 component2482799 1 BBa_K2042016 component2482798 1 BBa_K2042003 annotation2482800 1 BBa_K2042002 range2482800 1 14 1693 annotation2482799 1 BBa_K2042016 range2482799 1 8 13 annotation2482798 1 BBa_K2042003 range2482798 1 1 7 BBa_K2042010 1 BBa_K2042010 RBS + Pct 2016-09-01T11:00:00Z 2016-10-12T03:07:19Z a a false false _2509_ 31392 32278 9 false a false Jonathan Tellechea component2482791 1 BBa_K2042000 component2482789 1 BBa_K2042003 component2482790 1 BBa_K2042016 annotation2482789 1 BBa_K2042003 range2482789 1 1 7 annotation2482790 1 BBa_K2042016 range2482790 1 8 13 annotation2482791 1 BBa_K2042000 range2482791 1 14 1588 BBa_K2042016 1 BBa_K2042016 Non-coding scar site in between RBS and gene 2016-09-10T11:00:00Z 2016-10-08T02:48:20Z Synthetic synthesis Nucleotide between RBS and Pct false false _2509_ 31392 31392 9 false None false Toky RATOVOMANANA annotation2488301 1 scar range2488301 1 1 6 BBa_K2042017 1 BBa_K2042017 Ligation scar 2016-09-10T11:00:00Z 2016-10-12T03:22:34Z None Ligation scar false false _2509_ 31392 31392 9 false None false Toky RATOVOMANANA annotation2495255 1 Scar range2495255 1 1 5 BBa_K2042012_sequence 1 gaaggagctgaggatgagtaacaagagtaacgatgagttgaagtatcaagcctctgaaaacaccttggggcttaatcctgtcgttgggctgcgtggaaaggatctactggcttctgctcgaatggtgcttaggcaggccatcaagcaaccggtgcacagcgtcaaacatgtcgcgcactttggtcttgaactcaagaacgtactgctgggtaaatccgggctgcaaccgaccagcgatgaccgtcgcttcgccgatccggcctggagccagaacccgctctataaacgttatttgcaaacctacctggcgtggcgcaaggaactccacgactggatcgatgaaagtaacctcgcccccaaggatgtggcgcgtgggcacttcgtgatcaacctcatgaccgatgccatggcgccgaccaacaccgcggccaacccggcggcagtcaaacgctttttcgaaaccggtggcaaaagcctgctcgacggcctctcgcacctggccaaggatctggtacacaacggcggcatgccgagccaggtcaacatgggtgcattcgaggtcggcaagagcctgggcgtgaccgaaggcgcggtggtgtttcgcaacgatgtgctggaactgatccagtacaagccgaccaccgagcaggtatacgaacgcccgctgctggtggtgccgccgcagatcaacaagttctacgttttcgacctgagcccggacaagagcctggcgcggttctgcctgcgcaacaacgtgcaaacgttcatcgtcagctggcgaaatcccaccaaggaacagcgagagtggggcctgtcgacctacatcgaagccctcaaggaagcggttgacgtcgttaccgcgatcaccggcagcaaagacgtgaacatgctcggggcctgctccggcggcatcacttgcactgcgctgctgggccattacgcggcgattggcgaaaacaaggtcaacgccctgaccttgctggtgaccgtgcttgataccaccctcgacagcgacgtcgccctgttcgtcaatgaacagacccttgaagccgccaagcgccactcgtaccaggccggcgtactggaaggccgcgacatggcgaaggtcttcgcctggatgcgccccaacgatctgatctggaactactgggtcaacaattacctgctaggcaacgaaccgccggtgttcgacatcctgttctggaacaacgacaccacacggttgcccgcggcgttccacggcgacctggtcgaactgttcaaaaataacccactgattcgcccgaatgcactggaagtgtgcggcacccccatcgacctcaagcaggtgacggccgacatcttttccctggccggcaccaacgaccacatcaccccgtggaagtcctgctacaagtcggcgcaactgtttggcggcaacgttgaatttgtgctgtcgagcggcgggcatatcaagagcatcctgaacccgccgggcaatccgaaatcgcgctacatgaccagcaccgaagtggcggaaaatgccgatgaatggcaagcgaatgccaccaagcatacagattcctggtggctgcactggcaggcctggcaggcccaacgctcgggcgagctgaaaaagtccccgacaaaactgggcagcaaggcgtatccggcaggtgaagcggcgccaggcacgtacgtgcacgaacggtaa BBa_K2042016_sequence 1 ctgagg BBa_K2042002_sequence 1 atgagtaacaagagtaacgatgagttgaagtatcaagcctctgaaaacaccttggggcttaatcctgtcgttgggctgcgtggaaaggatctactggcttctgctcgaatggtgcttaggcaggccatcaagcaaccggtgcacagcgtcaaacatgtcgcgcactttggtcttgaactcaagaacgtactgctgggtaaatccgggctgcaaccgaccagcgatgaccgtcgcttcgccgatccggcctggagccagaacccgctctataaacgttatttgcaaacctacctggcgtggcgcaaggaactccacgactggatcgatgaaagtaacctcgcccccaaggatgtggcgcgtgggcacttcgtgatcaacctcatgaccgatgccatggcgccgaccaacaccgcggccaacccggcggcagtcaaacgctttttcgaaaccggtggcaaaagcctgctcgacggcctctcgcacctggccaaggatctggtacacaacggcggcatgccgagccaggtcaacatgggtgcattcgaggtcggcaagagcctgggcgtgaccgaaggcgcggtggtgtttcgcaacgatgtgctggaactgatccagtacaagccgaccaccgagcaggtatacgaacgcccgctgctggtggtgccgccgcagatcaacaagttctacgttttcgacctgagcccggacaagagcctggcgcggttctgcctgcgcaacaacgtgcaaacgttcatcgtcagctggcgaaatcccaccaaggaacagcgagagtggggcctgtcgacctacatcgaagccctcaaggaagcggttgacgtcgttaccgcgatcaccggcagcaaagacgtgaacatgctcggggcctgctccggcggcatcacttgcactgcgctgctgggccattacgcggcgattggcgaaaacaaggtcaacgccctgaccttgctggtgaccgtgcttgataccaccctcgacagcgacgtcgccctgttcgtcaatgaacagacccttgaagccgccaagcgccactcgtaccaggccggcgtactggaaggccgcgacatggcgaaggtcttcgcctggatgcgccccaacgatctgatctggaactactgggtcaacaattacctgctaggcaacgaaccgccggtgttcgacatcctgttctggaacaacgacaccacacggttgcccgcggcgttccacggcgacctggtcgaactgttcaaaaataacccactgattcgcccgaatgcactggaagtgtgcggcacccccatcgacctcaagcaggtgacggccgacatcttttccctggccggcaccaacgaccacatcaccccgtggaagtcctgctacaagtcggcgcaactgtttggcggcaacgttgaatttgtgctgtcgagcggcgggcatatcaagagcatcctgaacccgccgggcaatccgaaatcgcgctacatgaccagcaccgaagtggcggaaaatgccgatgaatggcaagcgaatgccaccaagcatacagattcctggtggctgcactggcaggcctggcaggcccaacgctcgggcgagctgaaaaagtccccgacaaaactgggcagcaaggcgtatccggcaggtgaagcggcgccaggcacgtacgtgcacgaacggtaa BBa_K2042014_sequence 1 gaaggagctgaggatgagtaacaagagtaacgatgagttgaagtatcaagcctctgaaaacaccttggggcttaatcctgtcgttgggctgcgtggaaaggatctactggcttctgctcgaatggtgcttaggcaggccatcaagcaaccggtgcacagcgtcaaacatgtcgcgcactttggtcttgaactcaagaacgtactgctgggtaaatccgggctgcaaccgaccagcgatgaccgtcgcttcgccgatccggcctggagccagaacccgctctataaacgttatttgcaaacctacctggcgtggcgcaaggaactccacgactggatcgatgaaagtaacctcgcccccaaggatgtggcgcgtgggcacttcgtgatcaacctcatgaccgatgccatggcgccgaccaacaccgcggccaacccggcggcagtcaaacgctttttcgaaaccggtggcaaaagcctgctcgacggcctctcgcacctggccaaggatctggtacacaacggcggcatgccgagccaggtcaacatgggtgcattcgaggtcggcaagagcctgggcgtgaccgaaggcgcggtggtgtttcgcaacgatgtgctggaactgatccagtacaagccgaccaccgagcaggtatacgaacgcccgctgctggtggtgccgccgcagatcaacaagttctacgttttcgacctgagcccggacaagagcctggcgcggttctgcctgcgcaacaacgtgcaaacgttcatcgtcagctggcgaaatcccaccaaggaacagcgagagtggggcctgtcgacctacatcgaagccctcaaggaagcggttgacgtcgttaccgcgatcaccggcagcaaagacgtgaacatgctcggggcctgctccggcggcatcacttgcactgcgctgctgggccattacgcggcgattggcgaaaacaaggtcaacgccctgaccttgctggtgaccgtgcttgataccaccctcgacagcgacgtcgccctgttcgtcaatgaacagacccttgaagccgccaagcgccactcgtaccaggccggcgtactggaaggccgcgacatggcgaaggtcttcgcctggatgcgccccaacgatctgatctggaactactgggtcaacaattacctgctaggcaacgaaccgccggtgttcgacatcctgttctggaacaacgacaccacacggttgcccgcggcgttccacggcgacctggtcgaactgttcaaaaataacccactgattcgcccgaatgcactggaagtgtgcggcacccccatcgacctcaagcaggtgacggccgacatcttttccctggccggcaccaacgaccacatcaccccgtggaagtcctgctacaagtcggcgcaactgtttggcggcaacgttgaatttgtgctgtcgagcggcgggcatatcaagagcatcctgaacccgccgggcaatccgaaatcgcgctacatgaccagcaccgaagtggcggaaaatgccgatgaatggcaagcgaatgccaccaagcatacagattcctggtggctgcactggcaggcctggcaggcccaacgctcgggcgagctgaaaaagtccccgacaaaactgggcagcaaggcgtatccggcaggtgaagcggcgccaggcacgtacgtgcacgaacggtaatactagaaggagctgaggatgcgtaaggtccctatcattaccgctgacgaagccgcgaaactgatcaaagatggggacacggtcaccaccagcggctttgtcggcaatgccattccggaggcgctggatcgcgctgtcgaaaaacgctttttggaaacgggcgagcccaaaaacatcacgtatgtgtactgtggttcccagggcaaccgcgacggtcggggcgcggagcattttgctcatgaaggcttgttgaagcgctatattgcggggcattgggctacggtcccggctctcgggaagatggcgatggagaacaaaatggaggcatacaatgtgtcgcagggggcactctgtcatctgttccgggatatcgcctcgcataagcccggcgtgtttacgaaagtgggcatcggtaccttcatcgaccctcggaacgggggcggtaaagtgaacgacatcacgaaagaagatatcgtcgaactggtcgaaatcaaggggcaggaatatttgttctaccccgcgtttcccatccatgtggcgctgatccgtggtacgtatgcggacgaatccggtaacattaccttcgagaaggaggtcgccccattggaaggcacgtccgtctgccaagcagtgaaaaactcgggggggatcgtcgtggtgcaagtggaacgggtcgtcaaagcgggcaccctggatccgcggcatgtcaaggtgccaggcatctacgtggactacgtcgtggtcaccgacccggaggatcaccaacagtcgctcgactgtgaatatgatccagcgctctccggcgaacaccggcgcccggaggtggtgggcgaacccctgcctctgtccgctaagaaagtcattggtcgtcgcggcgccattgagttggagaaagacgtcgccgtcaacctgggtgtcggtgcacccgagtacgtcgcgagcgtcgctgatgaggagggtattgtggatttcatgaccctgaccgctgaaagcggtgcaattggcggcgtcccagcgggtggcgtgcgcttcggtgcgtcctataatgcggacgccctgatcgatcaaggctatcagttcgattactatgatggcggtggtctcgatctctgttatctcgggttggctgaatgcgatgaaaaaggcaatattaacgtcagccgcttcggtccgcgcatcgccgggtgcggtggtttcattaatatcacccaaaacacccccaaggtgtttttttgtgggacgtttacggcgggcggtctgaaggtgaaaatcgaagatggtaaagtgatcatcgtccaggaggggaagcagaaaaagttcttgaaagccgtggagcagatcacgttcaacggggacgtggctctcgcaaataaacagcaagtcacgtacattacggagcgttgtgtcttcttgttgaaggaggatggtctgcacctcagcgaaatcgcgcctggtattgatctccagacccaaatcttggacgtgatggacttcgcaccgatcatcgaccgggatgcgaacgggcagatcaagctgatggacgctgcgctgtttgcagaagggctgatgggtctcaaggaaatgaaatcctga BBa_K2042017_sequence 1 tacta BBa_K2042000_sequence 1 atgcgtaaggtccctatcattaccgctgacgaagccgcgaaactgatcaaagatggggacacggtcaccaccagcggctttgtcggcaatgccattccggaggcgctggatcgcgctgtcgaaaaacgctttttggaaacgggcgagcccaaaaacatcacgtatgtgtactgtggttcccagggcaaccgcgacggtcggggcgcggagcattttgctcatgaaggcttgttgaagcgctatattgcggggcattgggctacggtcccggctctcgggaagatggcgatggagaacaaaatggaggcatacaatgtgtcgcagggggcactctgtcatctgttccgggatatcgcctcgcataagcccggcgtgtttacgaaagtgggcatcggtaccttcatcgaccctcggaacgggggcggtaaagtgaacgacatcacgaaagaagatatcgtcgaactggtcgaaatcaaggggcaggaatatttgttctaccccgcgtttcccatccatgtggcgctgatccgtggtacgtatgcggacgaatccggtaacattaccttcgagaaggaggtcgccccattggaaggcacgtccgtctgccaagcagtgaaaaactcgggggggatcgtcgtggtgcaagtggaacgggtcgtcaaagcgggcaccctggatccgcggcatgtcaaggtgccaggcatctacgtggactacgtcgtggtcaccgacccggaggatcaccaacagtcgctcgactgtgaatatgatccagcgctctccggcgaacaccggcgcccggaggtggtgggcgaacccctgcctctgtccgctaagaaagtcattggtcgtcgcggcgccattgagttggagaaagacgtcgccgtcaacctgggtgtcggtgcacccgagtacgtcgcgagcgtcgctgatgaggagggtattgtggatttcatgaccctgaccgctgaaagcggtgcaattggcggcgtcccagcgggtggcgtgcgcttcggtgcgtcctataatgcggacgccctgatcgatcaaggctatcagttcgattactatgatggcggtggtctcgatctctgttatctcgggttggctgaatgcgatgaaaaaggcaatattaacgtcagccgcttcggtccgcgcatcgccgggtgcggtggtttcattaatatcacccaaaacacccccaaggtgtttttttgtgggacgtttacggcgggcggtctgaaggtgaaaatcgaagatggtaaagtgatcatcgtccaggaggggaagcagaaaaagttcttgaaagccgtggagcagatcacgttcaacggggacgtggctctcgcaaataaacagcaagtcacgtacattacggagcgttgtgtcttcttgttgaaggaggatggtctgcacctcagcgaaatcgcgcctggtattgatctccagacccaaatcttggacgtgatggacttcgcaccgatcatcgaccgggatgcgaacgggcagatcaagctgatggacgctgcgctgtttgcagaagggctgatgggtctcaaggaaatgaaatcctga BBa_K2042003_sequence 1 gaaggag BBa_K2042010_sequence 1 gaaggagctgaggatgcgtaaggtccctatcattaccgctgacgaagccgcgaaactgatcaaagatggggacacggtcaccaccagcggctttgtcggcaatgccattccggaggcgctggatcgcgctgtcgaaaaacgctttttggaaacgggcgagcccaaaaacatcacgtatgtgtactgtggttcccagggcaaccgcgacggtcggggcgcggagcattttgctcatgaaggcttgttgaagcgctatattgcggggcattgggctacggtcccggctctcgggaagatggcgatggagaacaaaatggaggcatacaatgtgtcgcagggggcactctgtcatctgttccgggatatcgcctcgcataagcccggcgtgtttacgaaagtgggcatcggtaccttcatcgaccctcggaacgggggcggtaaagtgaacgacatcacgaaagaagatatcgtcgaactggtcgaaatcaaggggcaggaatatttgttctaccccgcgtttcccatccatgtggcgctgatccgtggtacgtatgcggacgaatccggtaacattaccttcgagaaggaggtcgccccattggaaggcacgtccgtctgccaagcagtgaaaaactcgggggggatcgtcgtggtgcaagtggaacgggtcgtcaaagcgggcaccctggatccgcggcatgtcaaggtgccaggcatctacgtggactacgtcgtggtcaccgacccggaggatcaccaacagtcgctcgactgtgaatatgatccagcgctctccggcgaacaccggcgcccggaggtggtgggcgaacccctgcctctgtccgctaagaaagtcattggtcgtcgcggcgccattgagttggagaaagacgtcgccgtcaacctgggtgtcggtgcacccgagtacgtcgcgagcgtcgctgatgaggagggtattgtggatttcatgaccctgaccgctgaaagcggtgcaattggcggcgtcccagcgggtggcgtgcgcttcggtgcgtcctataatgcggacgccctgatcgatcaaggctatcagttcgattactatgatggcggtggtctcgatctctgttatctcgggttggctgaatgcgatgaaaaaggcaatattaacgtcagccgcttcggtccgcgcatcgccgggtgcggtggtttcattaatatcacccaaaacacccccaaggtgtttttttgtgggacgtttacggcgggcggtctgaaggtgaaaatcgaagatggtaaagtgatcatcgtccaggaggggaagcagaaaaagttcttgaaagccgtggagcagatcacgttcaacggggacgtggctctcgcaaataaacagcaagtcacgtacattacggagcgttgtgtcttcttgttgaaggaggatggtctgcacctcagcgaaatcgcgcctggtattgatctccagacccaaatcttggacgtgatggacttcgcaccgatcatcgaccgggatgcgaacgggcagatcaagctgatggacgctgcgctgtttgcagaagggctgatgggtctcaaggaaatgaaatcctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z