##gff-version 3
##sequence-region BBa_K2042014 1 3286
BBa_K2042014	.	restriction_enzyme_assembly_scar	1694	1698	.	+	0	ID=BBa_K2042017;Name=BBa_K2042017
BBa_K2042014	.	null	1694	1698	.	+	0	ID=annotation2495255;Name=Scar;Parent=BBa_K2042017
BBa_K2042014	.	engineered_region	1	1693	.	+	0	ID=BBa_K2042012;Name=BBa_K2042012
BBa_K2042014	.	CDS	14	1693	.	+	0	ID=BBa_K2042002;Name=BBa_K2042002;Parent=BBa_K2042012
BBa_K2042014	.	sequence_alteration	1442	1442	.	+	0	ID=annotation2512501;Name=S477G;Parent=BBa_K2042002
BBa_K2042014	.	stop_codon	1691	1693	.	+	0	ID=annotation2512477;Name=Stop;Parent=BBa_K2042002
BBa_K2042014	.	start_codon	14	16	.	+	0	ID=annotation2512476;Name=Start;Parent=BBa_K2042002
BBa_K2042014	.	sequence_alteration	1454	1454	.	+	0	ID=annotation2512500;Name=Q481K;Parent=BBa_K2042002
BBa_K2042014	.	point_mutation	987	987	.	+	0	ID=annotation2512499;Name=Pst1 removed;Parent=BBa_K2042002
BBa_K2042014	.	sequence_alteration	987	987	.	+	0	ID=annotation2512503;Name=S325T;Parent=BBa_K2042002
BBa_K2042014	.	point_mutation	1429	1429	.	+	0	ID=annotation2512502;Name=Pst1 removed;Parent=BBa_K2042002
BBa_K2042014	.	CDS	14	1693	.	+	0	ID=annotation2512474;Name=PhaC;Parent=BBa_K2042002
BBa_K2042014	.	sequence_alteration	403	403	.	+	0	ID=annotation2512498;Name=E130D;Parent=BBa_K2042002
BBa_K2042014	.	ribosome_entry_site	1	7	.	+	0	ID=BBa_K2042003;Name=BBa_K2042003;Parent=BBa_K2042012
BBa_K2042014	.	ribosome_entry_site	1	7	.	+	0	ID=annotation2512505;Name=RBS;Parent=BBa_K2042003
BBa_K2042014	.	restriction_enzyme_assembly_scar	8	13	.	+	0	ID=BBa_K2042016;Name=BBa_K2042016;Parent=BBa_K2042012
BBa_K2042014	.	null	8	13	.	+	0	ID=annotation2488301;Name=scar;Parent=BBa_K2042016
BBa_K2042014	.	engineered_region	1699	3286	.	+	0	ID=BBa_K2042010;Name=BBa_K2042010
BBa_K2042014	.	ribosome_entry_site	1699	1705	.	+	0	ID=BBa_K2042003;Name=BBa_K2042003;Parent=BBa_K2042010
BBa_K2042014	.	ribosome_entry_site	1699	1705	.	+	0	ID=annotation2512505;Name=RBS;Parent=BBa_K2042003
BBa_K2042014	.	restriction_enzyme_assembly_scar	1706	1711	.	+	0	ID=BBa_K2042016;Name=BBa_K2042016;Parent=BBa_K2042010
BBa_K2042014	.	null	1706	1711	.	+	0	ID=annotation2488301;Name=scar;Parent=BBa_K2042016
BBa_K2042014	.	CDS	1712	3286	.	+	0	ID=BBa_K2042000;Name=BBa_K2042000;Parent=BBa_K2042010
BBa_K2042014	.	point_mutation	3157	3158	.	+	0	ID=annotation2512231;Name=s_mutation;Parent=BBa_K2042000
BBa_K2042014	.	CDS	1712	3286	.	+	0	ID=annotation2512227;Name=Pct;Parent=BBa_K2042000
BBa_K2042014	.	start_codon	1712	1714	.	+	0	ID=annotation2512228;Name=Start;Parent=BBa_K2042000
BBa_K2042014	.	sequence_alteration	2438	2439	.	+	0	ID=annotation2512235;Name=mutation;Parent=BBa_K2042000
BBa_K2042014	.	stop_codon	3284	3286	.	+	0	ID=annotation2512229;Name=Stop;Parent=BBa_K2042000
>BBa_K2042014
gaaggagctgaggatgagtaacaagagtaacgatgagttgaagtatcaagcctctgaaaacaccttggggcttaatcct
gtcgttgggctgcgtggaaaggatctactggcttctgctcgaatggtgcttaggcaggccatcaagcaaccggtgcaca
gcgtcaaacatgtcgcgcactttggtcttgaactcaagaacgtactgctgggtaaatccgggctgcaaccgaccagcga
tgaccgtcgcttcgccgatccggcctggagccagaacccgctctataaacgttatttgcaaacctacctggcgtggcgc
aaggaactccacgactggatcgatgaaagtaacctcgcccccaaggatgtggcgcgtgggcacttcgtgatcaacctca
tgaccgatgccatggcgccgaccaacaccgcggccaacccggcggcagtcaaacgctttttcgaaaccggtggcaaaag
cctgctcgacggcctctcgcacctggccaaggatctggtacacaacggcggcatgccgagccaggtcaacatgggtgca
ttcgaggtcggcaagagcctgggcgtgaccgaaggcgcggtggtgtttcgcaacgatgtgctggaactgatccagtaca
agccgaccaccgagcaggtatacgaacgcccgctgctggtggtgccgccgcagatcaacaagttctacgttttcgacct
gagcccggacaagagcctggcgcggttctgcctgcgcaacaacgtgcaaacgttcatcgtcagctggcgaaatcccacc
aaggaacagcgagagtggggcctgtcgacctacatcgaagccctcaaggaagcggttgacgtcgttaccgcgatcaccg
gcagcaaagacgtgaacatgctcggggcctgctccggcggcatcacttgcactgcgctgctgggccattacgcggcgat
tggcgaaaacaaggtcaacgccctgaccttgctggtgaccgtgcttgataccaccctcgacagcgacgtcgccctgttc
gtcaatgaacagacccttgaagccgccaagcgccactcgtaccaggccggcgtactggaaggccgcgacatggcgaagg
tcttcgcctggatgcgccccaacgatctgatctggaactactgggtcaacaattacctgctaggcaacgaaccgccggt
gttcgacatcctgttctggaacaacgacaccacacggttgcccgcggcgttccacggcgacctggtcgaactgttcaaa
aataacccactgattcgcccgaatgcactggaagtgtgcggcacccccatcgacctcaagcaggtgacggccgacatct
tttccctggccggcaccaacgaccacatcaccccgtggaagtcctgctacaagtcggcgcaactgtttggcggcaacgt
tgaatttgtgctgtcgagcggcgggcatatcaagagcatcctgaacccgccgggcaatccgaaatcgcgctacatgacc
agcaccgaagtggcggaaaatgccgatgaatggcaagcgaatgccaccaagcatacagattcctggtggctgcactggc
aggcctggcaggcccaacgctcgggcgagctgaaaaagtccccgacaaaactgggcagcaaggcgtatccggcaggtga
agcggcgccaggcacgtacgtgcacgaacggtaatactagaaggagctgaggatgcgtaaggtccctatcattaccgct
gacgaagccgcgaaactgatcaaagatggggacacggtcaccaccagcggctttgtcggcaatgccattccggaggcgc
tggatcgcgctgtcgaaaaacgctttttggaaacgggcgagcccaaaaacatcacgtatgtgtactgtggttcccaggg
caaccgcgacggtcggggcgcggagcattttgctcatgaaggcttgttgaagcgctatattgcggggcattgggctacg
gtcccggctctcgggaagatggcgatggagaacaaaatggaggcatacaatgtgtcgcagggggcactctgtcatctgt
tccgggatatcgcctcgcataagcccggcgtgtttacgaaagtgggcatcggtaccttcatcgaccctcggaacggggg
cggtaaagtgaacgacatcacgaaagaagatatcgtcgaactggtcgaaatcaaggggcaggaatatttgttctacccc
gcgtttcccatccatgtggcgctgatccgtggtacgtatgcggacgaatccggtaacattaccttcgagaaggaggtcg
ccccattggaaggcacgtccgtctgccaagcagtgaaaaactcgggggggatcgtcgtggtgcaagtggaacgggtcgt
caaagcgggcaccctggatccgcggcatgtcaaggtgccaggcatctacgtggactacgtcgtggtcaccgacccggag
gatcaccaacagtcgctcgactgtgaatatgatccagcgctctccggcgaacaccggcgcccggaggtggtgggcgaac
ccctgcctctgtccgctaagaaagtcattggtcgtcgcggcgccattgagttggagaaagacgtcgccgtcaacctggg
tgtcggtgcacccgagtacgtcgcgagcgtcgctgatgaggagggtattgtggatttcatgaccctgaccgctgaaagc
ggtgcaattggcggcgtcccagcgggtggcgtgcgcttcggtgcgtcctataatgcggacgccctgatcgatcaaggct
atcagttcgattactatgatggcggtggtctcgatctctgttatctcgggttggctgaatgcgatgaaaaaggcaatat
taacgtcagccgcttcggtccgcgcatcgccgggtgcggtggtttcattaatatcacccaaaacacccccaaggtgttt
ttttgtgggacgtttacggcgggcggtctgaaggtgaaaatcgaagatggtaaagtgatcatcgtccaggaggggaagc
agaaaaagttcttgaaagccgtggagcagatcacgttcaacggggacgtggctctcgcaaataaacagcaagtcacgta
cattacggagcgttgtgtcttcttgttgaaggaggatggtctgcacctcagcgaaatcgcgcctggtattgatctccag
acccaaatcttggacgtgatggacttcgcaccgatcatcgaccgggatgcgaacgggcagatcaagctgatggacgctg
cgctgtttgcagaagggctgatgggtctcaaggaaatgaaatcctga