BBa_K2042014
type: ComponentDefinition
persistent identity: BBa K2042014
display id: BBa_K2042014
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)
created: 2016-10-03T11:00:00Z
modified: 2016-10-14T03:28:59Z
mutable provenance: Enter before 2016 deadline
owned by: james
owned by: myers
mutable description: RBS + Pct + RBS + PhaC_v4
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31381
owner id: 31392
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Enter before 2016 deadline
star: false
creator: Toky RATOVOMANANA
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K2042010
component: BBa_K2042017
component: BBa_K2042012
sequence annotation: BBa_K2042012
sequence annotation: BBa_K2042017
sequence annotation: BBa_K2042010
sequence: https://synbiohub.org/public/igem/BBa_K2042014_sequence/1

BBa_K2042010
type: Component
persistent identity: component2485996
display id: component2485996
version: 1
top level: BBa_K2042014
definition: BBa_K2042010
access: public

BBa_K2042017
type: Component
persistent identity: component2485992
display id: component2485992
version: 1
top level: BBa_K2042014
definition: BBa_K2042017
access: public

BBa_K2042012
type: Component
persistent identity: component2485991
display id: component2485991
version: 1
top level: BBa_K2042014
definition: BBa_K2042012
access: public

BBa_K2042012
type: SequenceAnnotation
persistent identity: annotation2485991
display id: annotation2485991
version: 1
top level: BBa_K2042014
location: https://synbiohub.org/public/igem/BBa_K2042014/annotation2485991/range2485991/1
component: BBa_K2042012

https://synbiohub.org/public/igem/BBa_K2042014/annotation2485991/range2485991/1
type: Range
persistent identity: range2485991
display id: range2485991
version: 1
top level: BBa_K2042014
direction: direction/reverse
start: 1
end: 1693
orientation: inline

BBa_K2042017
type: SequenceAnnotation
persistent identity: annotation2485992
display id: annotation2485992
version: 1
top level: BBa_K2042014
location: https://synbiohub.org/public/igem/BBa_K2042014/annotation2485992/range2485992/1
component: BBa_K2042017

https://synbiohub.org/public/igem/BBa_K2042014/annotation2485992/range2485992/1
type: Range
persistent identity: range2485992
display id: range2485992
version: 1
top level: BBa_K2042014
direction: direction/reverse
start: 1694
end: 1698
orientation: inline

BBa_K2042010
type: SequenceAnnotation
persistent identity: annotation2485996
display id: annotation2485996
version: 1
top level: BBa_K2042014
location: https://synbiohub.org/public/igem/BBa_K2042014/annotation2485996/range2485996/1
component: BBa_K2042010

https://synbiohub.org/public/igem/BBa_K2042014/annotation2485996/range2485996/1
type: Range
persistent identity: range2485996
display id: range2485996
version: 1
top level: BBa_K2042014
direction: direction/reverse
start: 1699
end: 3286
orientation: inline

BBa_K2042010
type: ComponentDefinition
persistent identity: BBa K2042010
display id: BBa_K2042010
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + Pct
created: 2016-09-01T11:00:00Z
modified: 2016-10-12T03:07:19Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 32278
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Jonathan Tellechea
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K2042016
component: BBa_K2042003
component: BBa_K2042000
sequence annotation: BBa_K2042003
sequence annotation: BBa_K2042016
sequence annotation: BBa_K2042000
sequence: https://synbiohub.org/public/igem/BBa_K2042010_sequence/1

BBa_K2042016
type: Component
persistent identity: component2482790
display id: component2482790
version: 1
top level: BBa_K2042010
definition: BBa_K2042016
access: public

BBa_K2042003
type: Component
persistent identity: component2482789
display id: component2482789
version: 1
top level: BBa_K2042010
definition: BBa_K2042003
access: public

BBa_K2042000
type: Component
persistent identity: component2482791
display id: component2482791
version: 1
top level: BBa_K2042010
definition: Pct
access: public

BBa_K2042003
type: SequenceAnnotation
persistent identity: annotation2482789
display id: annotation2482789
version: 1
top level: BBa_K2042010
location: https://synbiohub.org/public/igem/BBa_K2042010/annotation2482789/range2482789/1
component: BBa_K2042003

https://synbiohub.org/public/igem/BBa_K2042010/annotation2482789/range2482789/1
type: Range
persistent identity: range2482789
display id: range2482789
version: 1
top level: BBa_K2042010
direction: direction/reverse
start: 1
end: 7
orientation: inline

BBa_K2042016
type: SequenceAnnotation
persistent identity: annotation2482790
display id: annotation2482790
version: 1
top level: BBa_K2042010
location: https://synbiohub.org/public/igem/BBa_K2042010/annotation2482790/range2482790/1
component: BBa_K2042016

https://synbiohub.org/public/igem/BBa_K2042010/annotation2482790/range2482790/1
type: Range
persistent identity: range2482790
display id: range2482790
version: 1
top level: BBa_K2042010
direction: direction/reverse
start: 8
end: 13
orientation: inline

BBa_K2042000
type: SequenceAnnotation
persistent identity: annotation2482791
display id: annotation2482791
version: 1
top level: BBa_K2042010
location: https://synbiohub.org/public/igem/BBa_K2042010/annotation2482791/range2482791/1
component: BBa_K2042000

https://synbiohub.org/public/igem/BBa_K2042010/annotation2482791/range2482791/1
type: Range
persistent identity: range2482791
display id: range2482791
version: 1
top level: BBa_K2042010
direction: direction/reverse
start: 14
end: 1588
orientation: inline

BBa_K2042012
type: ComponentDefinition
persistent identity: BBa K2042012
display id: BBa_K2042012
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + PhaC_sp. MBEL 6-19
created: 2016-09-02T11:00:00Z
modified: 2016-10-10T09:14:57Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: RBS + PhaC_v4
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31381
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Delphine Dessaux
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K2042002
component: BBa_K2042016
component: BBa_K2042003
sequence annotation: BBa_K2042003
sequence annotation: BBa_K2042016
sequence annotation: BBa_K2042002
sequence: https://synbiohub.org/public/igem/BBa_K2042012_sequence/1

BBa_K2042002
type: Component
persistent identity: component2482800
display id: component2482800
version: 1
top level: BBa_K2042012
definition: BBa_K2042002
access: public

BBa_K2042016
type: Component
persistent identity: component2482799
display id: component2482799
version: 1
top level: BBa_K2042012
definition: BBa_K2042016
access: public

BBa_K2042003
type: Component
persistent identity: component2482798
display id: component2482798
version: 1
top level: BBa_K2042012
definition: BBa_K2042003
access: public

BBa_K2042003
type: SequenceAnnotation
persistent identity: annotation2482798
display id: annotation2482798
version: 1
top level: BBa_K2042012
location: https://synbiohub.org/public/igem/BBa_K2042012/annotation2482798/range2482798/1
component: BBa_K2042003

https://synbiohub.org/public/igem/BBa_K2042012/annotation2482798/range2482798/1
type: Range
persistent identity: range2482798
display id: range2482798
version: 1
top level: BBa_K2042012
direction: direction/reverse
start: 1
end: 7
orientation: inline

BBa_K2042016
type: SequenceAnnotation
persistent identity: annotation2482799
display id: annotation2482799
version: 1
top level: BBa_K2042012
location: https://synbiohub.org/public/igem/BBa_K2042012/annotation2482799/range2482799/1
component: BBa_K2042016

https://synbiohub.org/public/igem/BBa_K2042012/annotation2482799/range2482799/1
type: Range
persistent identity: range2482799
display id: range2482799
version: 1
top level: BBa_K2042012
direction: direction/reverse
start: 8
end: 13
orientation: inline

BBa_K2042002
type: SequenceAnnotation
persistent identity: annotation2482800
display id: annotation2482800
version: 1
top level: BBa_K2042012
location: https://synbiohub.org/public/igem/BBa_K2042012/annotation2482800/range2482800/1
component: BBa_K2042002

https://synbiohub.org/public/igem/BBa_K2042012/annotation2482800/range2482800/1
type: Range
persistent identity: range2482800
display id: range2482800
version: 1
top level: BBa_K2042012
direction: direction/reverse
start: 14
end: 1693
orientation: inline

BBa_K2042017
type: ComponentDefinition
persistent identity: BBa K2042017
display id: BBa_K2042017
version: 1
was generated by: iGEM to SBOL conversion
description: Ligation scar
created: 2016-09-10T11:00:00Z
modified: 2016-10-12T03:22:34Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: Ligation scar
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31392
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Toky RATOVOMANANA
type: DnaRegion
role: SO:0001953
role: partType/Scar
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K2042017_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation2495255
display id: annotation2495255
version: 1
top level: BBa_K2042017
location: https://synbiohub.org/public/igem/BBa_K2042017/annotation2495255/range2495255/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K2042017/annotation2495255/range2495255/1
type: Range
persistent identity: range2495255
display id: range2495255
version: 1
top level: BBa_K2042017
direction: direction/forward
start: 1
end: 5
orientation: inline

Pct
type: ComponentDefinition
persistent identity: BBa K2042000
display id: BBa_K2042000
version: 1
was generated by: iGEM to SBOL conversion
description: Propionate CoA transferase for P. putida
created: 2016-08-18T11:00:00Z
modified: 2016-10-15T05:56:21Z
mutable provenance: The wild type sequence is from Clostridium propionicum genome.
owned by: james
owned by: myers
mutable description: This is the DNA coding sequence for the enzyme Propionate CoA. The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA. Transferase. This mutated sequence has one amino acid substitution: A243T (compared to the wild type enzyme) and is codon optimized for Pseudomonas putida in order to produce (D)-Lactyl-CoA. It extend the BBa_K1211001 biobrick from Yale 2013 and have the same purpose.
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31392
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We use this enzyme as the first enzyme in the pathway to create PLA or Polylactic acid. Lactate can also act as acceptors for the enzyme. In our project, the enzyme will catalyze the reaction: acetyl-CoA + lactate <--> acetate + Lactyl-CoA.
star: false
creator: Toky RATOVOMANANA
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Pct
sequence annotation: Start
sequence annotation: mutation
sequence annotation: s_mutation
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K2042000_sequence/1

Pct
type: SequenceAnnotation
persistent identity: annotation2512227
display id: annotation2512227
version: 1
top level: Pct
location: https://synbiohub.org/public/igem/BBa_K2042000/annotation2512227/range2512227/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K2042000/annotation2512227/range2512227/1
type: Range
persistent identity: range2512227
display id: range2512227
version: 1
top level: Pct
direction: direction/forward
start: 1
end: 1575
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2512228
display id: annotation2512228
version: 1
top level: Pct
location: https://synbiohub.org/public/igem/BBa_K2042000/annotation2512228/range2512228/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K2042000/annotation2512228/range2512228/1
type: Range
persistent identity: range2512228
display id: range2512228
version: 1
top level: Pct
direction: direction/forward
start: 1
end: 3
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2512235
display id: annotation2512235
version: 1
top level: Pct
location: https://synbiohub.org/public/igem/BBa_K2042000/annotation2512235/range2512235/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K2042000/annotation2512235/range2512235/1
type: Range
persistent identity: range2512235
display id: range2512235
version: 1
top level: Pct
direction: direction/forward
start: 727
end: 728
orientation: inline

s_mutation
type: SequenceAnnotation
persistent identity: annotation2512231
display id: annotation2512231
version: 1
top level: Pct
location: https://synbiohub.org/public/igem/BBa_K2042000/annotation2512231/range2512231/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K2042000/annotation2512231/range2512231/1
type: Range
persistent identity: range2512231
display id: range2512231
version: 1
top level: Pct
direction: direction/forward
start: 1446
end: 1447
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2512229
display id: annotation2512229
version: 1
top level: Pct
location: https://synbiohub.org/public/igem/BBa_K2042000/annotation2512229/range2512229/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K2042000/annotation2512229/range2512229/1
type: Range
persistent identity: range2512229
display id: range2512229
version: 1
top level: Pct
direction: direction/forward
start: 1573
end: 1575
orientation: inline

BBa_K2042002
type: ComponentDefinition
persistent identity: BBa K2042002
display id: BBa_K2042002
version: 1
was generated by: iGEM to SBOL conversion
description: Pseudomonas sp. MBEL 6-19 PHA synthase 1
created: 2016-09-01T11:00:00Z
modified: 2016-10-15T02:38:07Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31380
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Maria Bargu??s i Ribera
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start
sequence annotation: PhaC
sequence annotation: E130D
sequence annotation: S325T
sequence annotation: Pst1 removed
sequence annotation: S477G
sequence annotation: Q481K
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K2042002_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2512476
display id: annotation2512476
version: 1
top level: BBa_K2042002
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512476/range2512476/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512476/range2512476/1
type: Range
persistent identity: range2512476
display id: range2512476
version: 1
top level: BBa_K2042002
direction: direction/forward
start: 1
end: 3
orientation: inline

PhaC
type: SequenceAnnotation
persistent identity: annotation2512474
display id: annotation2512474
version: 1
top level: BBa_K2042002
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512474/range2512474/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512474/range2512474/1
type: Range
persistent identity: range2512474
display id: range2512474
version: 1
top level: BBa_K2042002
direction: direction/forward
start: 1
end: 1680
orientation: inline

E130D
type: SequenceAnnotation
persistent identity: annotation2512498
display id: annotation2512498
version: 1
top level: BBa_K2042002
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512498/range2512498/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512498/range2512498/1
type: Range
persistent identity: range2512498
display id: range2512498
version: 1
top level: BBa_K2042002
direction: direction/forward
start: 390
end: 390
orientation: inline

S325T
type: SequenceAnnotation
persistent identity: annotation2512503
display id: annotation2512503
version: 1
top level: BBa_K2042002
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512503/range2512503/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512503/range2512503/1
type: Range
persistent identity: range2512503
display id: range2512503
version: 1
top level: BBa_K2042002
direction: direction/forward
start: 974
end: 974
orientation: inline

Pst1 removed
type: SequenceAnnotation
persistent identity: annotation2512499
display id: annotation2512499
version: 1
top level: BBa_K2042002
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512499/range2512499/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512499/range2512499/1
type: Range
persistent identity: range2512499
display id: range2512499
version: 1
top level: BBa_K2042002
direction: direction/forward
start: 974
end: 974
orientation: inline

Pst1 removed
type: SequenceAnnotation
persistent identity: annotation2512502
display id: annotation2512502
version: 1
top level: BBa_K2042002
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512502/range2512502/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512502/range2512502/1
type: Range
persistent identity: range2512502
display id: range2512502
version: 1
top level: BBa_K2042002
direction: direction/forward
start: 1416
end: 1416
orientation: inline

S477G
type: SequenceAnnotation
persistent identity: annotation2512501
display id: annotation2512501
version: 1
top level: BBa_K2042002
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512501/range2512501/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512501/range2512501/1
type: Range
persistent identity: range2512501
display id: range2512501
version: 1
top level: BBa_K2042002
direction: direction/forward
start: 1429
end: 1429
orientation: inline

Q481K
type: SequenceAnnotation
persistent identity: annotation2512500
display id: annotation2512500
version: 1
top level: BBa_K2042002
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512500/range2512500/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512500/range2512500/1
type: Range
persistent identity: range2512500
display id: range2512500
version: 1
top level: BBa_K2042002
direction: direction/forward
start: 1441
end: 1441
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2512477
display id: annotation2512477
version: 1
top level: BBa_K2042002
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512477/range2512477/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512477/range2512477/1
type: Range
persistent identity: range2512477
display id: range2512477
version: 1
top level: BBa_K2042002
direction: direction/forward
start: 1678
end: 1680
orientation: inline

BBa_K2042003
type: ComponentDefinition
persistent identity: BBa K2042003
display id: BBa_K2042003
version: 1
was generated by: iGEM to SBOL conversion
description: RBS
created: 2016-09-01T11:00:00Z
modified: 2016-09-11T05:02:27Z
mutable provenance: A
owned by: james
owned by: myers
mutable description: A
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31443
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: A
star: false
creator: Charlotte NAYRAC
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K2042003_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2512505
display id: annotation2512505
version: 1
top level: BBa_K2042003
location: https://synbiohub.org/public/igem/BBa_K2042003/annotation2512505/range2512505/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K2042003/annotation2512505/range2512505/1
type: Range
persistent identity: range2512505
display id: range2512505
version: 1
top level: BBa_K2042003
direction: direction/forward
start: 1
end: 7
orientation: inline

BBa_K2042016
type: ComponentDefinition
persistent identity: BBa K2042016
display id: BBa_K2042016
version: 1
was generated by: iGEM to SBOL conversion
description: Non-coding scar site in between RBS and gene
created: 2016-09-10T11:00:00Z
modified: 2016-10-08T02:48:20Z
mutable provenance: Synthetic synthesis
owned by: james
owned by: myers
mutable description: Nucleotide between RBS and Pct
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31392
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Toky RATOVOMANANA
type: DnaRegion
role: SO:0001953
role: partType/Scar
sequence annotation: scar
sequence: https://synbiohub.org/public/igem/BBa_K2042016_sequence/1

scar
type: SequenceAnnotation
persistent identity: annotation2488301
display id: annotation2488301
version: 1
top level: BBa_K2042016
location: https://synbiohub.org/public/igem/BBa_K2042016/annotation2488301/range2488301/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K2042016/annotation2488301/range2488301/1
type: Range
persistent identity: range2488301
display id: range2488301
version: 1
top level: BBa_K2042016
direction: direction/forward
start: 1
end: 6
orientation: inline

https://synbiohub.org/public/igem/BBa_K2042014_sequence/1
type: Sequence
persistent identity: BBa K2042014 sequence
display id: BBa_K2042014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaggagctgaggatgagtaacaagagtaacgatgagttgaagtatcaagcctctgaaaacaccttggggcttaatcctgtcgttgggctgcgtggaaaggatctactggcttctgctcgaatggtgcttaggcaggccatcaagcaaccggtgcacagcgtcaaacatgtcgcgcactttggtcttgaactcaagaacgtactgctgggtaaatccgggctgcaaccgaccagcgatgaccgtcgcttcgccgatccggcctggagccagaacccgctctataaacgttatttgcaaacctacctggcgtggcgcaaggaactccacgactggatcgatgaaagtaacctcgcccccaaggatgtggcgcgtgggcacttcgtgatcaacctcatgaccgatgccatggcgccgaccaacaccgcggccaacccggcggcagtcaaacgctttttcgaaaccggtggcaaaagcctgctcgacggcctctcgcacctggccaaggatctggtacacaacggcggcatgccgagccaggtcaacatgggtgcattcgaggtcggcaagagcctgggcgtgaccgaaggcgcggtggtgtttcgcaacgatgtgctggaactgatccagtacaagccgaccaccgagcaggtatacgaacgcccgctgctggtggtgccgccgcagatcaacaagttctacgttttcgacctgagcccggacaagagcctggcgcggttctgcctgcgcaacaacgtgcaaacgttcatcgtcagctggcgaaatcccaccaaggaacagcgagagtggggcctgtcgacctacatcgaagccctcaaggaagcggttgacgtcgttaccgcgatcaccggcagcaaagacgtgaacatgctcggggcctgctccggcggcatcacttgcactgcgctgctgggccattacgcggcgattggcgaaaacaaggtcaacgccctgaccttgctggtgaccgtgcttgataccaccctcgacagcgacgtcgccctgttcgtcaatgaacagacccttgaagccgccaagcgccactcgtaccaggccggcgtactggaaggccgcgacatggcgaaggtcttcgcctggatgcgccccaacgatctgatctggaactactgggtcaacaattacctgctaggcaacgaaccgccggtgttcgacatcctgttctggaacaacgacaccacacggttgcccgcggcgttccacggcgacctggtcgaactgttcaaaaataacccactgattcgcccgaatgcactggaagtgtgcggcacccccatcgacctcaagcaggtgacggccgacatcttttccctggccggcaccaacgaccacatcaccccgtggaagtcctgctacaagtcggcgcaactgtttggcggcaacgttgaatttgtgctgtcgagcggcgggcatatcaagagcatcctgaacccgccgggcaatccgaaatcgcgctacatgaccagcaccgaagtggcggaaaatgccgatgaatggcaagcgaatgccaccaagcatacagattcctggtggctgcactggcaggcctggcaggcccaacgctcgggcgagctgaaaaagtccccgacaaaactgggcagcaaggcgtatccggcaggtgaagcggcgccaggcacgtacgtgcacgaacggtaatactagaaggagctgaggatgcgtaaggtccctatcattaccgctgacgaagccgcgaaactgatcaaagatggggacacggtcaccaccagcggctttgtcggcaatgccattccggaggcgctggatcgcgctgtcgaaaaacgctttttggaaacgggcgagcccaaaaacatcacgtatgtgtactgtggttcccagggcaaccgcgacggtcggggcgcggagcattttgctcatgaaggcttgttgaagcgctatattgcggggcattgggctacggtcccggctctcgggaagatggcga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2042010_sequence/1
type: Sequence
persistent identity: BBa K2042010 sequence
display id: BBa_K2042010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaggagctgaggatgcgtaaggtccctatcattaccgctgacgaagccgcgaaactgatcaaagatggggacacggtcaccaccagcggctttgtcggcaatgccattccggaggcgctggatcgcgctgtcgaaaaacgctttttggaaacgggcgagcccaaaaacatcacgtatgtgtactgtggttcccagggcaaccgcgacggtcggggcgcggagcattttgctcatgaaggcttgttgaagcgctatattgcggggcattgggctacggtcccggctctcgggaagatggcgatggagaacaaaatggaggcatacaatgtgtcgcagggggcactctgtcatctgttccgggatatcgcctcgcataagcccggcgtgtttacgaaagtgggcatcggtaccttcatcgaccctcggaacgggggcggtaaagtgaacgacatcacgaaagaagatatcgtcgaactggtcgaaatcaaggggcaggaatatttgttctaccccgcgtttcccatccatgtggcgctgatccgtggtacgtatgcggacgaatccggtaacattaccttcgagaaggaggtcgccccattggaaggcacgtccgtctgccaagcagtgaaaaactcgggggggatcgtcgtggtgcaagtggaacgggtcgtcaaagcgggcaccctggatccgcggcatgtcaaggtgccaggcatctacgtggactacgtcgtggtcaccgacccggaggatcaccaacagtcgctcgactgtgaatatgatccagcgctctccggcgaacaccggcgcccggaggtggtgggcgaacccctgcctctgtccgctaagaaagtcattggtcgtcgcggcgccattgagttggagaaagacgtcgccgtcaacctgggtgtcggtgcacccgagtacgtcgcgagcgtcgctgatgaggagggtattgtggatttcatgaccctgaccgctgaaagcggtgcaattggcggcgtcccagcgggtggcgtgcgcttcggtgcgtcctataatgcggacgccctgatcgatcaaggctatcagttcgattactatgatggcggtggtctcgatctctgttatctcgggttggctgaatgcgatgaaaaaggcaatattaacgtcagccgcttcggtccgcgcatcgccgggtgcggtggtttcattaatatcacccaaaacacccccaaggtgtttttttgtgggacgtttacggcgggcggtctgaaggtgaaaatcgaagatggtaaagtgatcatcgtccaggaggggaagcagaaaaagttcttgaaagccgtggagcagatcacgttcaacggggacgtggctctcgcaaataaacagcaagtcacgtacattacggagcgttgtgtcttcttgttgaaggaggatggtctgcacctcagcgaaatcgcgcctggtattgatctccagacccaaatcttggacgtgatggacttcgcaccgatcatcgaccgggatgcgaacgggcagatcaagctgatggacgctgcgctgtttgcagaagggctgatgggtctcaaggaaatgaaatcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2042012_sequence/1
type: Sequence
persistent identity: BBa K2042012 sequence
display id: BBa_K2042012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaggagctgaggatgagtaacaagagtaacgatgagttgaagtatcaagcctctgaaaacaccttggggcttaatcctgtcgttgggctgcgtggaaaggatctactggcttctgctcgaatggtgcttaggcaggccatcaagcaaccggtgcacagcgtcaaacatgtcgcgcactttggtcttgaactcaagaacgtactgctgggtaaatccgggctgcaaccgaccagcgatgaccgtcgcttcgccgatccggcctggagccagaacccgctctataaacgttatttgcaaacctacctggcgtggcgcaaggaactccacgactggatcgatgaaagtaacctcgcccccaaggatgtggcgcgtgggcacttcgtgatcaacctcatgaccgatgccatggcgccgaccaacaccgcggccaacccggcggcagtcaaacgctttttcgaaaccggtggcaaaagcctgctcgacggcctctcgcacctggccaaggatctggtacacaacggcggcatgccgagccaggtcaacatgggtgcattcgaggtcggcaagagcctgggcgtgaccgaaggcgcggtggtgtttcgcaacgatgtgctggaactgatccagtacaagccgaccaccgagcaggtatacgaacgcccgctgctggtggtgccgccgcagatcaacaagttctacgttttcgacctgagcccggacaagagcctggcgcggttctgcctgcgcaacaacgtgcaaacgttcatcgtcagctggcgaaatcccaccaaggaacagcgagagtggggcctgtcgacctacatcgaagccctcaaggaagcggttgacgtcgttaccgcgatcaccggcagcaaagacgtgaacatgctcggggcctgctccggcggcatcacttgcactgcgctgctgggccattacgcggcgattggcgaaaacaaggtcaacgccctgaccttgctggtgaccgtgcttgataccaccctcgacagcgacgtcgccctgttcgtcaatgaacagacccttgaagccgccaagcgccactcgtaccaggccggcgtactggaaggccgcgacatggcgaaggtcttcgcctggatgcgccccaacgatctgatctggaactactgggtcaacaattacctgctaggcaacgaaccgccggtgttcgacatcctgttctggaacaacgacaccacacggttgcccgcggcgttccacggcgacctggtcgaactgttcaaaaataacccactgattcgcccgaatgcactggaagtgtgcggcacccccatcgacctcaagcaggtgacggccgacatcttttccctggccggcaccaacgaccacatcaccccgtggaagtcctgctacaagtcggcgcaactgtttggcggcaacgttgaatttgtgctgtcgagcggcgggcatatcaagagcatcctgaacccgccgggcaatccgaaatcgcgctacatgaccagcaccgaagtggcggaaaatgccgatgaatggcaagcgaatgccaccaagcatacagattcctggtggctgcactggcaggcctggcaggcccaacgctcgggcgagctgaaaaagtccccgacaaaactgggcagcaaggcgtatccggcaggtgaagcggcgccaggcacgtacgtgcacgaacggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2042017_sequence/1
type: Sequence
persistent identity: BBa K2042017 sequence
display id: BBa_K2042017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2042000_sequence/1
type: Sequence
persistent identity: BBa K2042000 sequence
display id: BBa_K2042000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaggtccctatcattaccgctgacgaagccgcgaaactgatcaaagatggggacacggtcaccaccagcggctttgtcggcaatgccattccggaggcgctggatcgcgctgtcgaaaaacgctttttggaaacgggcgagcccaaaaacatcacgtatgtgtactgtggttcccagggcaaccgcgacggtcggggcgcggagcattttgctcatgaaggcttgttgaagcgctatattgcggggcattgggctacggtcccggctctcgggaagatggcgatggagaacaaaatggaggcatacaatgtgtcgcagggggcactctgtcatctgttccgggatatcgcctcgcataagcccggcgtgtttacgaaagtgggcatcggtaccttcatcgaccctcggaacgggggcggtaaagtgaacgacatcacgaaagaagatatcgtcgaactggtcgaaatcaaggggcaggaatatttgttctaccccgcgtttcccatccatgtggcgctgatccgtggtacgtatgcggacgaatccggtaacattaccttcgagaaggaggtcgccccattggaaggcacgtccgtctgccaagcagtgaaaaactcgggggggatcgtcgtggtgcaagtggaacgggtcgtcaaagcgggcaccctggatccgcggcatgtcaaggtgccaggcatctacgtggactacgtcgtggtcaccgacccggaggatcaccaacagtcgctcgactgtgaatatgatccagcgctctccggcgaacaccggcgcccggaggtggtgggcgaacccctgcctctgtccgctaagaaagtcattggtcgtcgcggcgccattgagttggagaaagacgtcgccgtcaacctgggtgtcggtgcacccgagtacgtcgcgagcgtcgctgatgaggagggtattgtggatttcatgaccctgaccgctgaaagcggtgcaattggcggcgtcccagcgggtggcgtgcgcttcggtgcgtcctataatgcggacgccctgatcgatcaaggctatcagttcgattactatgatggcggtggtctcgatctctgttatctcgggttggctgaatgcgatgaaaaaggcaatattaacgtcagccgcttcggtccgcgcatcgccgggtgcggtggtttcattaatatcacccaaaacacccccaaggtgtttttttgtgggacgtttacggcgggcggtctgaaggtgaaaatcgaagatggtaaagtgatcatcgtccaggaggggaagcagaaaaagttcttgaaagccgtggagcagatcacgttcaacggggacgtggctctcgcaaataaacagcaagtcacgtacattacggagcgttgtgtcttcttgttgaaggaggatggtctgcacctcagcgaaatcgcgcctggtattgatctccagacccaaatcttggacgtgatggacttcgcaccgatcatcgaccgggatgcgaacgggcagatcaagctgatggacgctgcgctgtttgcagaagggctgatgggtctcaaggaaatgaaatcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2042002_sequence/1
type: Sequence
persistent identity: BBa K2042002 sequence
display id: BBa_K2042002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtaacaagagtaacgatgagttgaagtatcaagcctctgaaaacaccttggggcttaatcctgtcgttgggctgcgtggaaaggatctactggcttctgctcgaatggtgcttaggcaggccatcaagcaaccggtgcacagcgtcaaacatgtcgcgcactttggtcttgaactcaagaacgtactgctgggtaaatccgggctgcaaccgaccagcgatgaccgtcgcttcgccgatccggcctggagccagaacccgctctataaacgttatttgcaaacctacctggcgtggcgcaaggaactccacgactggatcgatgaaagtaacctcgcccccaaggatgtggcgcgtgggcacttcgtgatcaacctcatgaccgatgccatggcgccgaccaacaccgcggccaacccggcggcagtcaaacgctttttcgaaaccggtggcaaaagcctgctcgacggcctctcgcacctggccaaggatctggtacacaacggcggcatgccgagccaggtcaacatgggtgcattcgaggtcggcaagagcctgggcgtgaccgaaggcgcggtggtgtttcgcaacgatgtgctggaactgatccagtacaagccgaccaccgagcaggtatacgaacgcccgctgctggtggtgccgccgcagatcaacaagttctacgttttcgacctgagcccggacaagagcctggcgcggttctgcctgcgcaacaacgtgcaaacgttcatcgtcagctggcgaaatcccaccaaggaacagcgagagtggggcctgtcgacctacatcgaagccctcaaggaagcggttgacgtcgttaccgcgatcaccggcagcaaagacgtgaacatgctcggggcctgctccggcggcatcacttgcactgcgctgctgggccattacgcggcgattggcgaaaacaaggtcaacgccctgaccttgctggtgaccgtgcttgataccaccctcgacagcgacgtcgccctgttcgtcaatgaacagacccttgaagccgccaagcgccactcgtaccaggccggcgtactggaaggccgcgacatggcgaaggtcttcgcctggatgcgccccaacgatctgatctggaactactgggtcaacaattacctgctaggcaacgaaccgccggtgttcgacatcctgttctggaacaacgacaccacacggttgcccgcggcgttccacggcgacctggtcgaactgttcaaaaataacccactgattcgcccgaatgcactggaagtgtgcggcacccccatcgacctcaagcaggtgacggccgacatcttttccctggccggcaccaacgaccacatcaccccgtggaagtcctgctacaagtcggcgcaactgtttggcggcaacgttgaatttgtgctgtcgagcggcgggcatatcaagagcatcctgaacccgccgggcaatccgaaatcgcgctacatgaccagcaccgaagtggcggaaaatgccgatgaatggcaagcgaatgccaccaagcatacagattcctggtggctgcactggcaggcctggcaggcccaacgctcgggcgagctgaaaaagtccccgacaaaactgggcagcaaggcgtatccggcaggtgaagcggcgccaggcacgtacgtgcacgaacggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2042003_sequence/1
type: Sequence
persistent identity: BBa K2042003 sequence
display id: BBa_K2042003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2042016_sequence/1
type: Sequence
persistent identity: BBa K2042016 sequence
display id: BBa_K2042016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgagg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z