RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)
Propionate CoA transferase for P. putida
The wild type sequence is from Clostridium propionicum genome.
This is the DNA coding sequence for the enzyme Propionate CoA. The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA. Transferase. This mutated sequence has one amino acid substitution: A243T (compared to the wild type enzyme) and is codon optimized for Pseudomonas putida in order to produce (D)-Lactyl-CoA. It extend the BBa_K1211001 biobrick from Yale 2013 and have the same purpose.
We use this enzyme as the first enzyme in the pathway to create PLA or Polylactic acid. Lactate can also act as acceptors for the enzyme. In our project, the enzyme will catalyze the reaction: acetyl-CoA + lactate <--> acetate + Lactyl-CoA.
Pseudomonas sp. MBEL 6-19 PHA synthase 1
Non-coding scar site in between RBS and gene
Conversion of the iGEM parts registry to SBOL2.1