BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_K2042015
1
BBa_K2042015
LDH* Generator
2016-08-29T11:00:00Z
2016-10-10T09:23:20Z
synthetic synthesis
Pputida promotor+igemrbs+ldh+terminqtor
false
false
_2509_
31392
31409
9
false
plamid pseva224 for increasing lactate
false
Amir Pandi
component2482888
1
BBa_K2042008
component2482887
1
BBa_K1583054
component2482883
1
BBa_K2042009
component2482882
1
BBa_K2042018
component2482895
1
BBa_B0015
component2482884
1
BBa_K2042019
component2482885
1
BBa_J34801
annotation2482887
1
BBa_K1583054
range2482887
1
64
69
annotation2482882
1
BBa_K2042018
range2482882
1
1
4
annotation2482895
1
BBa_B0015
range2482895
1
1072
1200
annotation2482885
1
BBa_J34801
range2482885
1
52
63
annotation2482888
1
BBa_K2042008
range2482888
1
70
1071
annotation2482884
1
BBa_K2042019
range2482884
1
45
51
annotation2482883
1
BBa_K2042009
range2482883
1
5
44
BBa_K2042009
1
BBa_K2042009
P. putida constitutive promoter
2016-08-29T11:00:00Z
2016-10-10T09:20:26Z
synthetic synthesis
It is the coding of pseudomonas putida constitutive promotor
false
false
_2509_
31392
31409
9
false
This promotor will constitutively express LDH
false
Amir Pandi
annotation2488299
1
pTN8-P1
range2488299
1
1
40
BBa_K2042008
1
D-LDH*
Engineered D-Lactate dehydrogenase (D-LDH*)
2016-08-29T11:00:00Z
2016-10-08T11:58:59Z
Synthetic synthesis
This is the coding sequence of lactate deshydrogenase that can super efficiently use NADPH and NADH as cofactor
false
false
_2509_
31392
31409
9
false
This enwy;e is for increasing lactate
false
Amir Pandi
annotation2512511
1
Start
range2512511
1
1
3
annotation2512513
1
Stop
range2512513
1
1000
1002
annotation2512510
1
D-LDH*
range2512510
1
1
1002
BBa_J34801
1
BBa_J34801
ribosome binding site
2006-10-25T11:00:00Z
2015-08-31T04:08:46Z
a
Released HQ 2013
a
false
false
_62_
0
1085
62
In stock
false
a
false
Alexandra Choutko
BBa_K1583054
1
BBa_K1583054
Non-coding scar site in between RBS and gene
2015-09-03T11:00:00Z
2015-09-18T08:03:55Z
The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655.
The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006.
CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA.
false
false
_2000_
24478
24463
9
false
We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation.
false
Stefan Robert Marsden
annotation2475152
1
Scar
range2475152
1
1
6
BBa_K2042019
1
BBa_K2042019
Spacer in between promoter and coding sequence
2016-09-10T11:00:00Z
2016-10-10T09:56:27Z
None
between LDH prom and RBS
false
false
_2509_
31392
31392
9
false
None
false
Toky RATOVOMANANA
annotation2495251
1
Spacer
range2495251
1
1
7
BBa_K2042018
1
BBa_K2042018
Scar before LDH*constitutive promoter
2016-09-10T11:00:00Z
2016-10-10T09:40:44Z
None
Random nucleotide before LDH promotor
false
false
_2509_
31392
31392
9
false
None
false
Toky RATOVOMANANA
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K2042009_sequence
1
cgactccacttgaacaatgttgtggtaccatttaaaacta
BBa_K2042019_sequence
1
ggatccg
BBa_K2042018_sequence
1
tagc
BBa_K2042015_sequence
1
tagccgactccacttgaacaatgttgtggtaccatttaaaactaggatccgaaagaggagaaatactagatgaccaaaatcttcgcgtatgcgatccgtgaagatgagaaaccctttctcaaggagtgggaggatgcccataaagatgtcgaggtcgagtacacggataagctcctcacgcctgaaaccgtggcattggccaagggggccgacggtgtggtcgtctatcagcagctcgattacacggcggaaaccttgcaagctttggcggacaacggtatcaccaagatgtcgttgcgtaacgtgggggtcgataatattgacatggcgaaggcaaaagaattggggtttcaaattaccaatgtgccagtgtactccccgaacgcaatcgcagaacatgcggctattcaagcagctcgtatcttgcgccaggacaaggcgatggatgagaaagtggcacgccatgatctccgctgggctccaaccattggccgggaggtccgtgaccaagtggtcggggtgatcggcacgggtcacattggtcaagtcttcatgcagatcatggaagggtttggggccaaggtcattgcatacagccgcacgcggaacccagagctggagaagaaggggtattacgtggatagcctcgatgacttgtataaacaggccgacgtgatctccctccacgtgcccgatgtgccggccaatgtgcacatgatcaatgacgaatccatcgcaaaaatgaaacaagacgtggtcattgtgaatgtcagccgtgggcccctcgtcgataccgacgccgtcattcgcgggttggacagcgggaaaatcttcggctatgcaatggatgtctacgagggcgaggtcggtatcttcaatgaggactgggagggtaaagaatttcctgatgcacgcttggcggacctgattgcccgtcctaatgtgctggtcacccctcacacggcattttacaccacgcacgcagtgcgtaatatggtcgtgaaggctttcgacaacaacctcgaattggtggaggggaaggaagccgaaaccccagtcaaggtggggtgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J34801_sequence
1
aaagaggagaaa
BBa_K1583054_sequence
1
tactag
BBa_K2042008_sequence
1
atgaccaaaatcttcgcgtatgcgatccgtgaagatgagaaaccctttctcaaggagtgggaggatgcccataaagatgtcgaggtcgagtacacggataagctcctcacgcctgaaaccgtggcattggccaagggggccgacggtgtggtcgtctatcagcagctcgattacacggcggaaaccttgcaagctttggcggacaacggtatcaccaagatgtcgttgcgtaacgtgggggtcgataatattgacatggcgaaggcaaaagaattggggtttcaaattaccaatgtgccagtgtactccccgaacgcaatcgcagaacatgcggctattcaagcagctcgtatcttgcgccaggacaaggcgatggatgagaaagtggcacgccatgatctccgctgggctccaaccattggccgggaggtccgtgaccaagtggtcggggtgatcggcacgggtcacattggtcaagtcttcatgcagatcatggaagggtttggggccaaggtcattgcatacagccgcacgcggaacccagagctggagaagaaggggtattacgtggatagcctcgatgacttgtataaacaggccgacgtgatctccctccacgtgcccgatgtgccggccaatgtgcacatgatcaatgacgaatccatcgcaaaaatgaaacaagacgtggtcattgtgaatgtcagccgtgggcccctcgtcgataccgacgccgtcattcgcgggttggacagcgggaaaatcttcggctatgcaatggatgtctacgagggcgaggtcggtatcttcaatgaggactgggagggtaaagaatttcctgatgcacgcttggcggacctgattgcccgtcctaatgtgctggtcacccctcacacggcattttacaccacgcacgcagtgcgtaatatggtcgtgaaggctttcgacaacaacctcgaattggtggaggggaaggaagccgaaaccccagtcaaggtggggtga
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z