Double terminator consisting of BBa_B0010 and BBa_B0012
Non-coding scar site in between RBS and gene
The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655. The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006.
CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA.
We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation.
Engineered D-Lactate dehydrogenase (D-LDH*)
This is the coding sequence of lactate deshydrogenase that can super efficiently use NADPH and NADH as cofactor
It is the coding of pseudomonas putida constitutive promotor
This promotor will constitutively express LDH
Spacer in between promoter and coding sequence
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1