BBa_K2045003 1 BBa_K2045003 CDC28-Cyclin-dependent kinase catalytic subunit 2016-10-13T11:00:00Z 2016-10-19T11:57:02Z S.cerevisiae mutant In order to have effective cell battle with our microgladiators??? It was important to ensure that cell division of our nanoparticle attached cells didn???t occur. Cell division would not only make it difficult for cell isolation in our microfluidic battle dome, but it will also compromise the integrity of the cell???s nanoweaponry; since, the coating of nanoparticles will be halved as the cell membrane goes through cell division. The solution was CDC28, a gene that allows users to control the growth of their cells by steadily controlled external temperature. The cells will only be active, cell division, at 24 degrees and inactive, restricted cell division, at 37 degrees. By attaching nanoparticles to our yeast cells with CDC28, and keeping them at 37 degrees in the microfluidic battle system, we can ensure an effective battle between our microgladiators without compromise of their attached nanoweapons. false false _2512_ 27450 27430 9 false CRISPR was needed to be used in order to effectively transform this useful mutant inti E.coli false Thiban Navaratnarajah BBa_K2045003_sequence 1 atgagcggtgaattagcaaattacaaaagacttgagaaagtcggtgaaggtacatacggtgttgtttataaagcgttagacttaagacctggccaaggtcaaagagtagtcgcattgaagaaaataagactagagagtgaagacgagggtgttcccagtacagccatcagagaaatctcattattgaaggaattaaaagacgataatattgtcagattatacgatattgttcactctgatgcacacaagctatatcttgtttttgagttcctcgatttggacctgaaaagatatatggagggtattccaaaggaccaaccgttaggagctgatattgttaagaagtttatgatgcaactttataagggtattgcatactgctactcacaccgtattctgcatcgtgatttaaaaccgcagaacttattgattaacaaagatgggaatctaaaactaggtgattttggcttagcgcgtgcttttggtgttccgttgagagcttacacacatgaaattgttactctatggtatagagctccggaggtattactgggtggaaaacaatatagtacaggtgtcgatacatggtccatcggctgtatatttgccgaaatgtgtaacaggaaaccaatcttcagtggcgatagtgagatcgatcagattttcaagatattcagagtattgggaacgccgaatgaagctatatggccagatattgtctacttgcctgatttcaagccaagctttcctcaatggcgcagaaaagacctatcacaagtggtaccaagtcttgatccacgcgatattgatttgttggacaaactcctcgcatatgaccctattaaccagattagcgccagaagagcagccatctacccctacttccaagaatcataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z