BBa_K204502 1 BBa_K204502 pTet+RBS+FliH 2009-10-14T11:00:00Z 2015-05-08T01:11:23Z pTet+RBS+FliH pTet+RBS+FliH false false _304_ 0 5360 9 It's complicated false pTet+RBS+FliH false Uno Keisuke component2042765 1 BBa_R0040 component2042773 1 BBa_K204500 component2042771 1 BBa_B0034 annotation2042771 1 BBa_B0034 range2042771 1 63 74 annotation2042773 1 BBa_K204500 range2042773 1 81 788 annotation2042765 1 BBa_R0040 range2042765 1 1 54 BBa_K204500 1 BBa_K204500 FliH 2009-10-14T11:00:00Z 2015-05-08T01:11:23Z FliH FliH false false _304_ 0 5360 9 Not in stock false FliH false Uno Keisuke annotation2042760 1 FliH range2042760 1 1 708 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986787 1 -10 range1986787 1 43 48 annotation1986785 1 -35 range1986785 1 20 25 BBa_K204502_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgtctaatgaattgccgtggcaagtctggacccccgacgatctcgccccgccgccagaaacttttgtgccggttgaggctgataacgtgaccttgacagaagatacgccggagcccgaactcactgccgaacagcagctggagcaggagctggcgcagctaaaaatacaggcccatgaacaaggttataacgccgggctggcggagggacggcaaaaaggacacgcgcagggataccaggaagggttagcgcaggggctggagcaaggacaagcccaggcgcagacgcagcaggcgccgattcatgcccgtatgcagcagttagtgagcgagtttcagaacacgcttgacgcgctcgatagcgttatcgcctcacggctgatgcaaatggccctggaggccgcacgtcaggtcatcggccaaacgccggcggtggacaactcagcgctgattaaacagatccaacaactgttgcagcaagagccgctctttagcggcaagccgcagttgcgcgtgcatccggacgacttacagcgggtggaagagatgctgggcgccacgctcagtcttcacggatggcggttacgcggcgatccaacgttgcatcacggcgggtgtaaagtctctgccgatgagggcgatctggacgccagcgtcgccactcgctggcaagagttgtgtcgcctggcggcgccgggagtgctctga BBa_K204500_sequence 1 atgtctaatgaattgccgtggcaagtctggacccccgacgatctcgccccgccgccagaaacttttgtgccggttgaggctgataacgtgaccttgacagaagatacgccggagcccgaactcactgccgaacagcagctggagcaggagctggcgcagctaaaaatacaggcccatgaacaaggttataacgccgggctggcggagggacggcaaaaaggacacgcgcagggataccaggaagggttagcgcaggggctggagcaaggacaagcccaggcgcagacgcagcaggcgccgattcatgcccgtatgcagcagttagtgagcgagtttcagaacacgcttgacgcgctcgatagcgttatcgcctcacggctgatgcaaatggccctggaggccgcacgtcaggtcatcggccaaacgccggcggtggacaactcagcgctgattaaacagatccaacaactgttgcagcaagagccgctctttagcggcaagccgcagttgcgcgtgcatccggacgacttacagcgggtggaagagatgctgggcgccacgctcagtcttcacggatggcggttacgcggcgatccaacgttgcatcacggcgggtgtaaagtctctgccgatgagggcgatctggacgccagcgtcgccactcgctggcaagagttgtgtcgcctggcggcgccgggagtgctctga BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z