BBa_C0171 1 rhIR rhlR repressor/activator from P. aeruginosa PA3477 (no LVA) 2004-05-26T11:00:00Z 2015-08-31T04:07:24Z Released HQ 2013 same as C0071 except no LVA tag false false _11_1_ 0 61 7 In stock false true jcbraff BBa_K204504 1 BBa_K204504 RhlR receiver+FliH 2009-10-14T11:00:00Z 2015-05-08T01:11:23Z pTetR+RBS+RhlI+terminater+pRhlR+FliH pTetR+RBS+RhlI+terminater+pRhlR+FliH false false _304_ 0 5360 9 It's complicated false pTetR+RBS+RhlI+terminater+pRhlR+FliH false Uno Keisuke component2049490 1 BBa_K204500 component2049477 1 BBa_B0010 component2049476 1 BBa_C0171 component2049469 1 BBa_R0040 component2049479 1 BBa_B0012 component2049488 1 BBa_B0034 component2049475 1 BBa_B0034 component2049486 1 BBa_I14017 annotation2049476 1 BBa_C0171 range2049476 1 81 809 annotation2049477 1 BBa_B0010 range2049477 1 818 897 annotation2049475 1 BBa_B0034 range2049475 1 63 74 annotation2049469 1 BBa_R0040 range2049469 1 1 54 annotation2049490 1 BBa_K204500 range2049490 1 1032 1739 annotation2049486 1 BBa_I14017 range2049486 1 955 1005 annotation2049479 1 BBa_B0012 range2049479 1 906 946 annotation2049488 1 BBa_B0034 range2049488 1 1014 1025 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K204500 1 BBa_K204500 FliH 2009-10-14T11:00:00Z 2015-05-08T01:11:23Z FliH FliH false false _304_ 0 5360 9 Not in stock false FliH false Uno Keisuke annotation2042760 1 FliH range2042760 1 1 708 BBa_I14017 1 RhI P(Rhl) 2004-08-01T11:00:00Z 2015-08-31T04:07:37Z Released HQ 2013 RhlR and C4HSL regulated promoter. false true _4_ 0 171 7 In stock false true Vikram Vijayan, Allen Hsu, Lawrence Fomundam annotation1043007 1 -35 range1043007 1 21 50 annotation1043005 1 RhlR range1043005 1 1 20 annotation1043006 1 modified P(Rhl) range1043006 1 1 51 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K204500_sequence 1 atgtctaatgaattgccgtggcaagtctggacccccgacgatctcgccccgccgccagaaacttttgtgccggttgaggctgataacgtgaccttgacagaagatacgccggagcccgaactcactgccgaacagcagctggagcaggagctggcgcagctaaaaatacaggcccatgaacaaggttataacgccgggctggcggagggacggcaaaaaggacacgcgcagggataccaggaagggttagcgcaggggctggagcaaggacaagcccaggcgcagacgcagcaggcgccgattcatgcccgtatgcagcagttagtgagcgagtttcagaacacgcttgacgcgctcgatagcgttatcgcctcacggctgatgcaaatggccctggaggccgcacgtcaggtcatcggccaaacgccggcggtggacaactcagcgctgattaaacagatccaacaactgttgcagcaagagccgctctttagcggcaagccgcagttgcgcgtgcatccggacgacttacagcgggtggaagagatgctgggcgccacgctcagtcttcacggatggcggttacgcggcgatccaacgttgcatcacggcgggtgtaaagtctctgccgatgagggcgatctggacgccagcgtcgccactcgctggcaagagttgtgtcgcctggcggcgccgggagtgctctga BBa_B0034_sequence 1 aaagaggagaaa BBa_C0171_sequence 1 atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K204504_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgcatgaattggtaggcttacgcaagaaaatggtttgttatagtcgaatactagagaaagaggagaaatactagatgtctaatgaattgccgtggcaagtctggacccccgacgatctcgccccgccgccagaaacttttgtgccggttgaggctgataacgtgaccttgacagaagatacgccggagcccgaactcactgccgaacagcagctggagcaggagctggcgcagctaaaaatacaggcccatgaacaaggttataacgccgggctggcggagggacggcaaaaaggacacgcgcagggataccaggaagggttagcgcaggggctggagcaaggacaagcccaggcgcagacgcagcaggcgccgattcatgcccgtatgcagcagttagtgagcgagtttcagaacacgcttgacgcgctcgatagcgttatcgcctcacggctgatgcaaatggccctggaggccgcacgtcaggtcatcggccaaacgccggcggtggacaactcagcgctgattaaacagatccaacaactgttgcagcaagagccgctctttagcggcaagccgcagttgcgcgtgcatccggacgacttacagcgggtggaagagatgctgggcgccacgctcagtcttcacggatggcggttacgcggcgatccaacgttgcatcacggcgggtgtaaagtctctgccgatgagggcgatctggacgccagcgtcgccactcgctggcaagagttgtgtcgcctggcggcgccgggagtgctctga BBa_I14017_sequence 1 tcctgcatgaattggtaggcttacgcaagaaaatggtttgttatagtcgaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z