LOCUS       BBa_K204702             1889 bp    DNA     linear       16-Dec-2024
DEFINITION  RhlR+GFP
ACCESSION   BBa_K204702
VERSION     BBa_K204702.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..809
                     /label=BBa_C0171
     stem_loop       829..872
                     /label=stem_loop
     misc_feature    818..897
                     /label=BBa_B0010
     terminator      818..897
                     /label=BBa_B0010
     stem_loop       913..932
                     /label=T7 TE
     misc_feature    939..939
                     /label=stop
     polyA_site      933..946
                     /label=polya
     misc_feature    906..946
                     /label=BBa_B0012
     terminator      906..946
                     /label=BBa_B0012
     misc_feature    955..974
                     /label=RhlR
     misc_feature    955..1005
                     /label=modified P(Rhl)
     promoter        975..1004
                     /label=-35
     promoter        955..1005
                     /label=BBa_I14017
     RBS             1014..1026
                     /label=RBS-3\Weak
     RBS             1020..1023
                     /label=RBS
     misc_feature    1014..1026
                     /label=BBa_B0032
     RBS             1014..1026
                     /label=BBa_B0032
     CDS             1033..1752
                     /label=GFP protein
     CDS             1033..1752
                     /label=BBa_E0040
     stem_loop       1772..1815
                     /label=stem_loop
     misc_feature    1761..1840
                     /label=BBa_B0010
     terminator      1761..1840
                     /label=BBa_B0010
     stem_loop       1856..1875
                     /label=T7 TE
     misc_feature    1882..1882
                     /label=stop
     polyA_site      1876..1889
                     /label=polya
     misc_feature    1849..1889
                     /label=BBa_B0012
     terminator      1849..1889
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaggaatg acggaggctt tttgctgtgg tgggacggtt
      121 tgcgtagcga gatgcagccg atccacgaca gccagggcgt gttcgccgtc ctggaaaagg
      181 aagtgcggcg cctgggcttc gattactacg cctatggcgt gcgccacacg attcccttca
      241 cccggccgaa gaccgaggtc catggcacct atcccaaggc ctggctggag cgataccaga
      301 tgcagaacta cggggccgtg gatccggcga tcctcaacgg cctgcgctcc tcggaaatgg
      361 tggtctggag cgacagcctg ttcgaccaga gccggatgct ctggaacgag gctcgcgatt
      421 ggggcctctg tgtcggcgcg accttgccga tccgcgcgcc gaacaatttg ctcagcgtgc
      481 tttccgtggc gcgcgaccag cagaacatct ccagcttcga gcgcgaggaa atccgcctgc
      541 ggctgcgttg catgatcgag ttgctgaccc agaagctgac cgacctggag catccgatgc
      601 tgatgtccaa cccggtctgc ctgagccatc gcgaacgcga gatcctgcaa tggaccgccg
      661 acggcaagag ttccggggaa atcgccatca tcctgagcat ctccgagagc acggtgaact
      721 tccaccacaa gaacatccag aagaagttcg acgcgccgaa caagacgctg gctgccgcct
      781 acgccgcggc gctgggtctc atctaataat actagagcca ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      901 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcctgc
      961 atgaattggt aggcttacgc aagaaaatgg tttgttatag tcgaatacta gagtcacaca
     1021 ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1081 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1141 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1201 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1261 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1321 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1381 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1441 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1501 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1561 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1621 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1681 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     1741 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1801 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1861 accttcgggt gggcctttct gcgtttata
//