BBa_K2047011 1 BBa_K2047011 Stem-loop with free energy of -51.4 kcal/mol, measured by Mfold 2016-10-18T11:00:00Z 2016-10-21T12:35:39Z NO This sequence encodes a stem loop that can regulate multiple gene expression when inserted in intergenic region followed with a RNaseE site in E.coli. Different stem loops have different regulation effects and we quantitatively weighed this difference by their free energy evaluated by a software---Mfold .Of which the free energy of the most stable secondary structure of this sequence is -20.0 kcal/mol. false false _2514_ 30452 30452 9 false None false Yifei Li BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K2047105 1 BBa_K2047105 Fluorescent reporter system with stem loop of -51.4kcal/mol, GFP_Stem-loop 2016-10-13T11:00:00Z 2016-10-19T08:11:28Z no This sequence is used for measuring the regulation effect of stem loop of free energy -30.1 kcal/mol false false _2514_ 30452 30440 9 false none false Yu Jiang component2522109 1 BBa_K2047011 component2522108 1 BBa_E0040 annotation2522109 1 BBa_K2047011 range2522109 1 721 827 annotation2522108 1 BBa_E0040 range2522108 1 1 720 BBa_K2047011_sequence 1 gtcgacgatcgcctgatcccggtgcacccgggcagctgcagagtctgggtgcaccgggatcaggccgcggctagcgtgaaaatacgagaatattatttgtattgatc BBa_K2047105_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataagtcgacgatcgcctgatcccggtgcacccgggcagctgcagagtctgggtgcaccgggatcaggccgcggctagcgtgaaaatacgagaatattatttgtattgatc BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z