BBa_K2048003
1
BBa_K2048003
Short GolS
2016-10-12T11:00:00Z
2016-10-13T08:39:28Z
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
This composite part contains Tet promoter, teto,GolS. GolS is a MerR family transcriptional regulator, gold-responsive activator of gol and ges genes. GolB is a promoter that gets activated by GolS factor. This circuit produces golS under the regulation of TetO promoter.
false
false
_2515_
30815
30815
9
false
This circuit was designed to synthesize GolS under the Tet promoter regulation
false
Seray Cicek
BBa_K2048001
1
BBa_K2048001
Long GolS
2016-10-12T11:00:00Z
2016-10-14T11:08:59Z
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
This composite part contains Tet promoter, teto,GolS,GolB and LacZ genes. GolS is a MerR family transcriptional regulator, gold-responsive activator of gol and ges genes. GolB is a promoter that gets activated by GolS factor. This circuit produces golS under the regulation of TetO promoter. Synthesized GolS protein binds to GolB promoter and synthesizes LacZ which generates a visible color.
false
false
_2515_
0
30815
30815
9
It's complicated
false
Our main goal was to generate a circuit that allows production of GolS transcriptional factor and activate production of LacZ. This is achieved by placing golB before the LacZ region
false
Seray Cicek
component2507192
1
BBa_K2048006
component2507191
1
BBa_K2048003
annotation2507191
1
BBa_K2048003
range2507191
1
1
580
annotation2507192
1
BBa_K2048006
range2507192
1
581
917
BBa_K2048006
1
BBa_K2048006
Short GolB LacZ
2016-10-13T11:00:00Z
2016-10-14T10:37:20Z
Salmonella enterica serovar Typhimurium (S. Typhimurium)
This part contains a GolB promoter, and LacZ reporter. GolB is activated with transcriptional regulator GolS in the presence of gold and LacZ unit produces a color.
false
false
_2515_
30815
30815
9
false
This circuit is aimed to be active in the presence of gold and golS.
false
Seray Cicek
BBa_K2048003_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgacgtaacgcaaagggaggagagtaaaatgaacatcggtaaagcagctaaagcatcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggctatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctgaaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacattgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggcgacgcgctgccagattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcggtattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaacgcaaaaaaccccgcttcggcggggttttttcgc
BBa_K2048001_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgacgtaacgcaaagggaggagagtaaaatgaacatcggtaaagcagctaaagcatcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggctatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctgaaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacattgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggcgacgcgctgccagattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcggtattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaacgcaaaaaaccccgcttcggcggggttttttcgccttgaccttccaacactggcaaggtccagactggcaacaaaatcacgaaaatcaattaagggggtacaaatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataacgccgaaaaccccgcttcggcggggttttgccgc
BBa_K2048006_sequence
1
cttgaccttccaacactggcaaggtccagactggcaacaaaatcacgaaaatcaattaagggggtacaaatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataacgccgaaaaccccgcttcggcggggttttgccgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z