BBa_K2048002
1
BBa_K2048002
Long GolS (P118A)
2016-10-12T11:00:00Z
2016-10-14T11:40:58Z
Salmonella Typhimurium Mutant
This composite part contains Tet promoter, teto,GolS(P118A),GolB and LacZ genes. GolS is a MerR family transcriptional regulator, gold-responsive activator of gol and ges genes. This circuit contains mutant GolS P118A which is highly sensitice to gold. GolB is a promoter that gets activated by GolS factor. This circuit produces golS under the regulation of TetO promoter. Synthesized GolS protein binds to GolB promoter and synthesizes LacZ which generates a visible color.
false
false
_2515_
30815
30815
9
false
This circuit had same design considerations as BBa_K2048001. In addition, high sensitivity was introduced.
false
Seray Cicek
component2507580
1
BBa_K2048006
component2507579
1
BBa_K2048004
annotation2507580
1
BBa_K2048006
range2507580
1
589
925
annotation2507579
1
BBa_K2048004
range2507579
1
1
588
BBa_K2048004
1
BBa_K2048004
Short GolS (P118)
2016-10-13T11:00:00Z
2016-10-14T10:23:37Z
Salmonella enterica serovar Typhimurium (S. Typhimurium)
This cpart contains Tet promoter, tetO and GolS(P118A) genes. GolS is a MerR family transcriptional regulator, gold-responsive activator of gol and ges genes. GolB is a promoter that gets activated by GolS factor. GolS(P118A) is the mutant of GolS that is more sensitive to gold.
false
false
_2515_
30815
30815
9
false
This circuit contains a tetO promoter tetR RBS that producet the transcriptional factor GolS(P118)
false
Seray Cicek
BBa_K2048006
1
BBa_K2048006
Short GolB LacZ
2016-10-13T11:00:00Z
2016-10-14T10:37:20Z
Salmonella enterica serovar Typhimurium (S. Typhimurium)
This part contains a GolB promoter, and LacZ reporter. GolB is activated with transcriptional regulator GolS in the presence of gold and LacZ unit produces a color.
false
false
_2515_
30815
30815
9
false
This circuit is aimed to be active in the presence of gold and golS.
false
Seray Cicek
BBa_K2048004_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgccattataaacaattaaggataggaggtatattaatgaacatcggtaaagcagctaaagcatcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggctatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctgaaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacattgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggcgacgcgctggcggattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcggtattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaacgcaaaaaaccccgcttcggcggggttttttcgc
BBa_K2048002_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgccattataaacaattaaggataggaggtatattaatgaacatcggtaaagcagctaaagcatcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggctatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctgaaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacattgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggcgacgcgctggcggattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcggtattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaacgcaaaaaaccccgcttcggcggggttttttcgccttgaccttccaacactggcaaggtccagactggcaacaaaatcacgaaaatcaattaagggggtacaaatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataacgccgaaaaccccgcttcggcggggttttgccgc
BBa_K2048006_sequence
1
cttgaccttccaacactggcaaggtccagactggcaacaaaatcacgaaaatcaattaagggggtacaaatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataacgccgaaaaccccgcttcggcggggttttgccgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z