BBa_K2048002 1 BBa_K2048002 Long GolS (P118A) 2016-10-12T11:00:00Z 2016-10-14T11:40:58Z Salmonella Typhimurium Mutant This composite part contains Tet promoter, teto,GolS(P118A),GolB and LacZ genes. GolS is a MerR family transcriptional regulator, gold-responsive activator of gol and ges genes. This circuit contains mutant GolS P118A which is highly sensitice to gold. GolB is a promoter that gets activated by GolS factor. This circuit produces golS under the regulation of TetO promoter. Synthesized GolS protein binds to GolB promoter and synthesizes LacZ which generates a visible color. false false _2515_ 30815 30815 9 false This circuit had same design considerations as BBa_K2048001. In addition, high sensitivity was introduced. false Seray Cicek component2507579 1 BBa_K2048004 component2507580 1 BBa_K2048006 annotation2507579 1 BBa_K2048004 range2507579 1 1 588 annotation2507580 1 BBa_K2048006 range2507580 1 589 925 BBa_K2048004 1 BBa_K2048004 Short GolS (P118) 2016-10-13T11:00:00Z 2016-10-14T10:23:37Z Salmonella enterica serovar Typhimurium (S. Typhimurium) This cpart contains Tet promoter, tetO and GolS(P118A) genes. GolS is a MerR family transcriptional regulator, gold-responsive activator of gol and ges genes. GolB is a promoter that gets activated by GolS factor. GolS(P118A) is the mutant of GolS that is more sensitive to gold. false false _2515_ 30815 30815 9 false This circuit contains a tetO promoter tetR RBS that producet the transcriptional factor GolS(P118) false Seray Cicek BBa_K2048006 1 BBa_K2048006 Short GolB LacZ 2016-10-13T11:00:00Z 2016-10-14T10:37:20Z Salmonella enterica serovar Typhimurium (S. Typhimurium) This part contains a GolB promoter, and LacZ reporter. GolB is activated with transcriptional regulator GolS in the presence of gold and LacZ unit produces a color. false false _2515_ 30815 30815 9 false This circuit is aimed to be active in the presence of gold and golS. false Seray Cicek BBa_K2048004_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgccattataaacaattaaggataggaggtatattaatgaacatcggtaaagcagctaaagcatcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggctatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctgaaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacattgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggcgacgcgctggcggattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcggtattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaacgcaaaaaaccccgcttcggcggggttttttcgc BBa_K2048002_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgccattataaacaattaaggataggaggtatattaatgaacatcggtaaagcagctaaagcatcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggctatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctgaaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacattgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggcgacgcgctggcggattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcggtattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaacgcaaaaaaccccgcttcggcggggttttttcgccttgaccttccaacactggcaaggtccagactggcaacaaaatcacgaaaatcaattaagggggtacaaatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataacgccgaaaaccccgcttcggcggggttttgccgc BBa_K2048006_sequence 1 cttgaccttccaacactggcaaggtccagactggcaacaaaatcacgaaaatcaattaagggggtacaaatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataacgccgaaaaccccgcttcggcggggttttgccgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z