BBa_K2050421 1 BBa_K2050421 Thosea Asigna Virus 2A peptide linker (T2A) 2016-10-12T11:00:00Z 2016-10-13T10:06:20Z We looked up the amino acid sequence in Yang et al. (doi:10.1038/gt.2008.90) and Tang et al. (10.1523/JNEUROSCI.0359-09.2009), and then created the DNA sequence ourselves. 2A peptide is a self-cleaving peptide linker. The sequence encoding the 2A peptide is typically included between the coding sequences of two proteins without stop codons to co-express the two proteins in a single vector. The two coding sequences will be transcribed onto one mRNA molecule along with the coding sequence for the 2A peptide. During translation, however, the peptide will cleave the peptide bond between the glycine and the proline residue on its C-terminus. The result is two different polypeptide chains. The coding sequence for 2A peptide is far shorter than that of an IRES, making it preferable in constructing a multicistronic vector. The consequence of using the 2A peptide linker, however, is that the upstream protein will have additional residues from the 2A peptide on its C-terminus and the downstream protein will start with a proline. false false _2518_ 30522 30522 9 false The amino acid sequence was optimized for human expression to achieve maximum codon adaptation index possible (evaluated with GenScript's rare codon analysis tool) with considerations to its flanking sequences. false Muhammad Farhan Maruli BBa_K2050421_sequence 1 ggatccggagaaggcagaggcagcctgctgacatgtggagatgtggaagagaatcccgggccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z