LOCUS BBa_K2052014 1178 bp DNA linear 25-Nov-2024 DEFINITION FimH site directed mutated with RPMrel ACCESSION BBa_K2052014 VERSION BBa_K2052014.1 FEATURES Location/Qualifiers CDS 1..903 /label=fimH CDS 1..903 /label=BBa_K1850001 stem_loop 923..966 /label=stem_loop misc_feature 912..991 /label=BBa_B0010 terminator 912..991 /label=BBa_B0010 stem_loop 1007..1026 /label=T7 TE misc_feature 1033..1033 /label=stop polyA_site 1027..1040 /label=polya misc_feature 1000..1040 /label=BBa_B0012 terminator 1000..1040 /label=BBa_B0012 terminator 912..1040 /label=BBa_B0015 promoter 1049..1179 /label=promoter misc_feature 1088..1105 /label=AraI1 misc_feature 1109..1126 /label=AraI2 promoter 1049..1178 /label=BBa_K206000 ORIGIN 1 atgaaacgag ttattaccct gtttgctgta ctgctgatgg gctggtcggt aaatgcctgg 61 tcattcgcct gtaaaaccgc caatggtacc gctatcccta ttggcggtgg cagcgccaat 121 gtttatgtaa accttgcgcc cgtcgtgaat gtggggcaaa acctggtcgt ggatctttcg 181 acgcaaatct tttgccataa cgattatccg gaaaccatta cagactatgt cacactgcaa 241 cgaggctcgg cttatggcgg cgtgttatct aatttttccg ggaccgtaaa atatagtggc 301 agtagctatc catttcctac caccagcgaa acgccgcgcg ttgtttataa ttcgagaacg 361 gataagccgt ggccggtggc gctttatttg acgcctgtga gcagtgcggg cggggtggcg 421 attaaagctg gctcattaat tgccgtgctt attttgcgac agaccaacaa ctataacagc 481 gatgatttcc agtttgtgtg gaatatttac gccaataatg atgtggtggt gcctactggc 541 ggctgcgatg tttctgctcg tgatgtcacc gttactctgc cggactaccc tggttcagtg 601 ccaattcctc ttaccgttta ttgtgcgaaa agccaaaacc tggggtatta cctctccggc 661 acaaccgcag atgcgggcaa ctcgattttc accaataccg cgtcgttttc acctgcacag 721 ggcgtcggcg tacagttgac gcgcaacggt acgattattc cagcgaataa cacggtatcg 781 ttaggagcag tagggacttc ggcggtgagt ctgggattaa cggcaaatta tgcacgtacc 841 ggagggcagg tgactgcggg gaatgtgcaa tcgattattg gcgtgacttt tgtttatcaa 901 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 961 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 1021 tgggcctttc tgcgtttata tactagagac attgattatt tgcacggcgt cacactttgc 1081 tatgccatag caagatagtc cataagatta gcggatccta cctgacgctt tttatcgcaa 1141 ctctctactg tttctccata ccgttttttt gggctagc //