LOCUS       BBa_K2052016            2565 bp    DNA     linear       16-Jun-2024
DEFINITION  FimH site directed mutated with RPMrel and ButCoat
ACCESSION   BBa_K2052016
VERSION     BBa_K2052016.1  
FEATURES             Location/Qualifiers
     CDS             1..903
                     /label=fimH
     CDS             1..903
                     /label=BBa_K1850001
     stem_loop       923..966
                     /label=stem_loop
     misc_feature    912..991
                     /label=BBa_B0010
     terminator      912..991
                     /label=BBa_B0010
     stem_loop       1007..1026
                     /label=T7 TE
     misc_feature    1033..1033
                     /label=stop
     polyA_site      1027..1040
                     /label=polya
     misc_feature    1000..1040
                     /label=BBa_B0012
     terminator      1000..1040
                     /label=BBa_B0012
     terminator      912..1040
                     /label=BBa_B0015
     promoter        1049..1179
                     /label=promoter
     misc_feature    1088..1105
                     /label=AraI1
     misc_feature    1109..1126
                     /label=AraI2
     promoter        1049..1178
                     /label=BBa_K206000
     misc_feature    1191..1194
                     /label=conserved
     RBS             1187..1198
                     /label=BBa_B0034
     CDS             1205..2565
                     /label=BBa_K2052018
ORIGIN
        1 atgaaacgag ttattaccct gtttgctgta ctgctgatgg gctggtcggt aaatgcctgg
       61 tcattcgcct gtaaaaccgc caatggtacc gctatcccta ttggcggtgg cagcgccaat
      121 gtttatgtaa accttgcgcc cgtcgtgaat gtggggcaaa acctggtcgt ggatctttcg
      181 acgcaaatct tttgccataa cgattatccg gaaaccatta cagactatgt cacactgcaa
      241 cgaggctcgg cttatggcgg cgtgttatct aatttttccg ggaccgtaaa atatagtggc
      301 agtagctatc catttcctac caccagcgaa acgccgcgcg ttgtttataa ttcgagaacg
      361 gataagccgt ggccggtggc gctttatttg acgcctgtga gcagtgcggg cggggtggcg
      421 attaaagctg gctcattaat tgccgtgctt attttgcgac agaccaacaa ctataacagc
      481 gatgatttcc agtttgtgtg gaatatttac gccaataatg atgtggtggt gcctactggc
      541 ggctgcgatg tttctgctcg tgatgtcacc gttactctgc cggactaccc tggttcagtg
      601 ccaattcctc ttaccgttta ttgtgcgaaa agccaaaacc tggggtatta cctctccggc
      661 acaaccgcag atgcgggcaa ctcgattttc accaataccg cgtcgttttc acctgcacag
      721 ggcgtcggcg tacagttgac gcgcaacggt acgattattc cagcgaataa cacggtatcg
      781 ttaggagcag tagggacttc ggcggtgagt ctgggattaa cggcaaatta tgcacgtacc
      841 ggagggcagg tgactgcggg gaatgtgcaa tcgattattg gcgtgacttt tgtttatcaa
      901 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      961 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1021 tgggcctttc tgcgtttata tactagagac attgattatt tgcacggcgt cacactttgc
     1081 tatgccatag caagatagtc cataagatta gcggatccta cctgacgctt tttatcgcaa
     1141 ctctctactg tttctccata ccgttttttt gggctagcta ctagagaaag aggagaaata
     1201 ctagatgatc ttctacaact atgaggaagt gtttgttatg gactacagcc aggagtataa
     1261 gcaaaaactg gtgagcgcgg atgaagcggt taagctgatt aaaagcggtg actgggtgga
     1321 tttcggctgg tgcaccaaca ccgttgacgc gctggatcag gcgctggcga agcgtaccga
     1381 cgagctgacc aacgtgaacc tgcgtggtgg catcctgctg aaaccgctgg cgacctttgc
     1441 gcgtgaggat gcgggtgaac actttacctg gaacagctgg cacatgagcg gtatcgagcg
     1501 taagatgatt agccgtggct gcgcgttcta cagcccgatt cgttatagcg agctgccgcg
     1561 ttactatcgt gaactggact gcccggacga tgtggcgatg tttcaagttg cgccgatgga
     1621 tagccaccac tacttcaact ttggtccgag cgcgagccac ctgggtgcga tgtgcgagcg
     1681 tgcgaaacac atcattgttg aggtgaacga aaacatgccg cgttgcctgg gtggcaccga
     1741 atgcggtatc cacattagcg acgtgaccta tatcgttgag ggcagcaacc cgccgattgg
     1801 tgaactgggt gcgggtggcc cggcgaccga catcgataag accgtggcga aactgatcgt
     1861 tgatgagatt ccgaacggtg cgtgcctgca actgggtatc ggtggcatgc cgaacgcggt
     1921 gggtagcctg attgcggaga gcgacctgaa ggatctgggc gttcacaccg aaatgtacgt
     1981 ggacgcgttc gtggacatcg cgaaggcggg taaaattacc ggcgcgaaga aaaacatcga
     2041 ccgttaccgt caaacctatg cgtttggtgc gggcaccaag aaaatgtacg attatctgga
     2101 cgataacccg gagctgatga gcgcgccggt ggactacacc aacgacatcc gtagcattag
     2161 cgcgctggac aacttcatca gcattaacaa cgcggtggac atcgatctgt atggtcaggt
     2221 taacgcggaa agcgcgggta tcaaacagat tagcggcgcg ggtggccaac tggactttgt
     2281 tctgggtgcg tacctgagca agggtggcaa gagcttcatc tgcctgagca gcacctttaa
     2341 gaccaaagac ggtcaggtgc aaagccgtat ccgtccgacc ctggcgaacg gcagcattgt
     2401 taccgatacc cgtgcgaaca cccactacgt ggttaccgaa tatggcaagg tgaacgttaa
     2461 aggcctgagc gcgtggcaaa aggcggaggc gctgatcagc attgcgcacc cggactttcg
     2521 tgatgaactg atcaaagagg cggaaaaaat gcacatttgg cgtcg
//