BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K2052018 1 ButCoAT Butyryl-CoA:acetate CoA-transferase 2016-09-23T11:00:00Z 2016-10-16T06:29:12Z .................. Enter the source of this part. For example, does it come from some genomic sequence? ............. Enter a long description of the part so that users of your part know what it is, what it does, and how to use it in their projects. false false _2520_ 16902 16902 9 false ........... Enter any design considerations you had to deal with during the detailed design of the sequence. false Burak K&#305;z&#305;l, Seniz Yuksel BBa_K2052016 1 BBa_K2052016 FimH site directed mutated with RPMrel and ButCoat 2016-09-24T11:00:00Z 2016-10-19T06:33:52Z ............... Enter the source of this part. For example, does it come from some genomic sequence? ........................... Enter a long description of the part so that users of your part know what it is, what it does, and how to use it in their projects. false false _2520_ 16909 16902 9 false ..................... Enter any design considerations you had to deal with during the detailed design of the sequence. false Burak K&#305;z&#305;l, Seniz Yuksel component2484980 1 BBa_K1850001 component2484993 1 BBa_B0034 component2484991 1 BBa_K206000 component2484987 1 BBa_B0015 component2484994 1 BBa_K2052018 annotation2484993 1 BBa_B0034 range2484993 1 1187 1198 annotation2484987 1 BBa_B0015 range2484987 1 912 1040 annotation2484994 1 BBa_K2052018 range2484994 1 1205 2565 annotation2484980 1 BBa_K1850001 range2484980 1 1 903 annotation2484991 1 BBa_K206000 range2484991 1 1049 1178 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1850001 1 BBa_K1850001 fimH 2015-09-14T11:00:00Z 2015-09-15T07:27:53Z fish comes from E. coli K-12 genome fimH gene, useful for production of type 1 pili. false false _2277_ 26421 26421 9 false edited out illegal cut sites false Lydia Goldberg annotation2456833 1 fimH range2456833 1 1 903 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049254 1 AraI2 range2049254 1 61 78 annotation2049252 1 promoter range2049252 1 1 131 annotation2049253 1 AraI1 range2049253 1 40 57 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1850001_sequence 1 atgaaacgagttattaccctgtttgctgtactgctgatgggctggtcggtaaatgcctggtcattcgcctgtaaaaccgccaatggtaccgctatccctattggcggtggcagcgccaatgtttatgtaaaccttgcgcccgtcgtgaatgtggggcaaaacctggtcgtggatctttcgacgcaaatcttttgccataacgattatccggaaaccattacagactatgtcacactgcaacgaggctcggcttatggcggcgtgttatctaatttttccgggaccgtaaaatatagtggcagtagctatccatttcctaccaccagcgaaacgccgcgcgttgtttataattcgagaacggataagccgtggccggtggcgctttatttgacgcctgtgagcagtgcgggcggggtggcgattaaagctggctcattaattgccgtgcttattttgcgacagaccaacaactataacagcgatgatttccagtttgtgtggaatatttacgccaataatgatgtggtggtgcctactggcggctgcgatgtttctgctcgtgatgtcaccgttactctgccggactaccctggttcagtgccaattcctcttaccgtttattgtgcgaaaagccaaaacctggggtattacctctccggcacaaccgcagatgcgggcaactcgattttcaccaataccgcgtcgttttcacctgcacagggcgtcggcgtacagttgacgcgcaacggtacgattattccagcgaataacacggtatcgttaggagcagtagggacttcggcggtgagtctgggattaacggcaaattatgcacgtaccggagggcaggtgactgcggggaatgtgcaatcgattattggcgtgacttttgtttatcaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K2052018_sequence 1 atgatcttctacaactatgaggaagtgtttgttatggactacagccaggagtataagcaaaaactggtgagcgcggatgaagcggttaagctgattaaaagcggtgactgggtggatttcggctggtgcaccaacaccgttgacgcgctggatcaggcgctggcgaagcgtaccgacgagctgaccaacgtgaacctgcgtggtggcatcctgctgaaaccgctggcgacctttgcgcgtgaggatgcgggtgaacactttacctggaacagctggcacatgagcggtatcgagcgtaagatgattagccgtggctgcgcgttctacagcccgattcgttatagcgagctgccgcgttactatcgtgaactggactgcccggacgatgtggcgatgtttcaagttgcgccgatggatagccaccactacttcaactttggtccgagcgcgagccacctgggtgcgatgtgcgagcgtgcgaaacacatcattgttgaggtgaacgaaaacatgccgcgttgcctgggtggcaccgaatgcggtatccacattagcgacgtgacctatatcgttgagggcagcaacccgccgattggtgaactgggtgcgggtggcccggcgaccgacatcgataagaccgtggcgaaactgatcgttgatgagattccgaacggtgcgtgcctgcaactgggtatcggtggcatgccgaacgcggtgggtagcctgattgcggagagcgacctgaaggatctgggcgttcacaccgaaatgtacgtggacgcgttcgtggacatcgcgaaggcgggtaaaattaccggcgcgaagaaaaacatcgaccgttaccgtcaaacctatgcgtttggtgcgggcaccaagaaaatgtacgattatctggacgataacccggagctgatgagcgcgccggtggactacaccaacgacatccgtagcattagcgcgctggacaacttcatcagcattaacaacgcggtggacatcgatctgtatggtcaggttaacgcggaaagcgcgggtatcaaacagattagcggcgcgggtggccaactggactttgttctgggtgcgtacctgagcaagggtggcaagagcttcatctgcctgagcagcacctttaagaccaaagacggtcaggtgcaaagccgtatccgtccgaccctggcgaacggcagcattgttaccgatacccgtgcgaacacccactacgtggttaccgaatatggcaaggtgaacgttaaaggcctgagcgcgtggcaaaaggcggaggcgctgatcagcattgcgcacccggactttcgtgatgaactgatcaaagaggcggaaaaaatgcacatttggcgtcgtagcaacaaatat BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_K2052016_sequence 1 atgaaacgagttattaccctgtttgctgtactgctgatgggctggtcggtaaatgcctggtcattcgcctgtaaaaccgccaatggtaccgctatccctattggcggtggcagcgccaatgtttatgtaaaccttgcgcccgtcgtgaatgtggggcaaaacctggtcgtggatctttcgacgcaaatcttttgccataacgattatccggaaaccattacagactatgtcacactgcaacgaggctcggcttatggcggcgtgttatctaatttttccgggaccgtaaaatatagtggcagtagctatccatttcctaccaccagcgaaacgccgcgcgttgtttataattcgagaacggataagccgtggccggtggcgctttatttgacgcctgtgagcagtgcgggcggggtggcgattaaagctggctcattaattgccgtgcttattttgcgacagaccaacaactataacagcgatgatttccagtttgtgtggaatatttacgccaataatgatgtggtggtgcctactggcggctgcgatgtttctgctcgtgatgtcaccgttactctgccggactaccctggttcagtgccaattcctcttaccgtttattgtgcgaaaagccaaaacctggggtattacctctccggcacaaccgcagatgcgggcaactcgattttcaccaataccgcgtcgttttcacctgcacagggcgtcggcgtacagttgacgcgcaacggtacgattattccagcgaataacacggtatcgttaggagcagtagggacttcggcggtgagtctgggattaacggcaaattatgcacgtaccggagggcaggtgactgcggggaatgtgcaatcgattattggcgtgacttttgtttatcaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgatcttctacaactatgaggaagtgtttgttatggactacagccaggagtataagcaaaaactggtgagcgcggatgaagcggttaagctgattaaaagcggtgactgggtggatttcggctggtgcaccaacaccgttgacgcgctggatcaggcgctggcgaagcgtaccgacgagctgaccaacgtgaacctgcgtggtggcatcctgctgaaaccgctggcgacctttgcgcgtgaggatgcgggtgaacactttacctggaacagctggcacatgagcggtatcgagcgtaagatgattagccgtggctgcgcgttctacagcccgattcgttatagcgagctgccgcgttactatcgtgaactggactgcccggacgatgtggcgatgtttcaagttgcgccgatggatagccaccactacttcaactttggtccgagcgcgagccacctgggtgcgatgtgcgagcgtgcgaaacacatcattgttgaggtgaacgaaaacatgccgcgttgcctgggtggcaccgaatgcggtatccacattagcgacgtgacctatatcgttgagggcagcaacccgccgattggtgaactgggtgcgggtggcccggcgaccgacatcgataagaccgtggcgaaactgatcgttgatgagattccgaacggtgcgtgcctgcaactgggtatcggtggcatgccgaacgcggtgggtagcctgattgcggagagcgacctgaaggatctgggcgttcacaccgaaatgtacgtggacgcgttcgtggacatcgcgaaggcgggtaaaattaccggcgcgaagaaaaacatcgaccgttaccgtcaaacctatgcgtttggtgcgggcaccaagaaaatgtacgattatctggacgataacccggagctgatgagcgcgccggtggactacaccaacgacatccgtagcattagcgcgctggacaacttcatcagcattaacaacgcggtggacatcgatctgtatggtcaggttaacgcggaaagcgcgggtatcaaacagattagcggcgcgggtggccaactggactttgttctgggtgcgtacctgagcaagggtggcaagagcttcatctgcctgagcagcacctttaagaccaaagacggtcaggtgcaaagccgtatccgtccgaccctggcgaacggcagcattgttaccgatacccgtgcgaacacccactacgtggttaccgaatatggcaaggtgaacgttaaaggcctgagcgcgtggcaaaaggcggaggcgctgatcagcattgcgcacccggactttcgtgatgaactgatcaaagaggcggaaaaaatgcacatttggcgtcg BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z